Detailed information NTG0255411

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CTV95_RS22070
• Genome accession: NZ_CP024780
• Coordinates: 4863100..4863945 (+)
• Length: 281 a.a.
• NCBI ID: WP_100018428.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain BZA12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4858100..4868945

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CTV95_RS22040 (CTV95_22040)	gspI	4858492..4858857 (+)	366	WP_039273738.1	type II secretion system minor pseudopilin GspI	-
CTV95_RS22045 (CTV95_22045)	gspJ	4858857..4859507 (+)	651	WP_072010021.1	type II secretion system minor pseudopilin GspJ	-
CTV95_RS22050 (CTV95_22050)	gspK	4859504..4860490 (+)	987	WP_014916095.1	type II secretion system minor pseudopilin GspK	-
CTV95_RS22055 (CTV95_22055)	gspL	4860487..4861764 (+)	1278	WP_100018425.1	type II secretion system protein GspL	-
CTV95_RS22060 (CTV95_22060)	gspM	4861761..4862255 (+)	495	WP_100018426.1	type II secretion system protein GspM	-
CTV95_RS22065 (CTV95_22065)	-	4862245..4862991 (+)	747	WP_100018427.1	type II secretion system protein N	-
CTV95_RS22070 (CTV95_22070)	pilD	4863100..4863945 (+)	846	WP_100018428.1	prepilin peptidase	Machinery gene
CTV95_RS22075 (CTV95_22075)	ypdK	4864136..4864210 (-)	75	WP_099606229.1	membrane protein YpdK	-
CTV95_RS22080 (CTV95_22080)	-	4864568..4865593 (-)	1026	WP_014916091.1	LLM class flavin-dependent oxidoreductase	-
CTV95_RS22085 (CTV95_22085)	-	4865904..4866968 (+)	1065	WP_100018429.1	ABC transporter substrate-binding protein	-
CTV95_RS22090 (CTV95_22090)	-	4866965..4867984 (+)	1020	WP_100018430.1	FecCD family ABC transporter permease	-
CTV95_RS22095 (CTV95_22095)	-	4867986..4868756 (+)	771	WP_100018431.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31463.39 Da
• Isoelectric Point: 5.7656

>NTDB_id=255411 CTV95_RS22070 WP_100018428.1 4863100..4863945(+) (pilD) [Pectobacterium brasiliense strain BZA12]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMADSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSETFVPLNDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=255411 CTV95_RS22070 WP_100018428.1 4863100..4863945(+) (pilD) [Pectobacterium brasiliense strain BZA12]
GTGGACGATTTAAGGGAATTTGCACAGCTATTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTTATTTACCGCCTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGATATCGAGCTTGAAACGG
GCATGGCCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGTCCGCACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAGCTGGCTGTGGTTGCGCGGCCGCTCCCGCTGCTGTCATCAATCAGTATCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGCTGCTGTTTAATCTGTCGGAGACCTTTGTCCCATTGAATGATGCCGTAGTCGGCGC
GATGGCTGGTTATTTATCTCTGTGGCTACTTTATTGGGTATTCAAATACGCAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCACTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGTTGAGC
GGGTTGGTCGTGACTCTCGTTTGGCGCGGGTTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.185	96.441	0.484
pilD	Neisseria gonorrhoeae MS11	47.126	92.883	0.438
pilD	Vibrio campbellii strain DS40M4	44.981	95.73	0.431
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402