Detailed information NTG0255371

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: CUC43_RS15545
• Genome accession: NZ_CP024771
• Coordinates: 2889966..2893691 (+)
• Length: 1241 a.a.
• NCBI ID: WP_029437815.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis LM1212
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2884966..2898691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CUC43_RS15530 (CUC43_16025)	-	2885124..2885717 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
CUC43_RS15535 (CUC43_16030)	lepB	2885774..2886337 (+)	564	WP_000751904.1	signal peptidase I	-
CUC43_RS15540 (CUC43_16035)	addB	2886454..2889969 (+)	3516	WP_029437814.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
CUC43_RS15545 (CUC43_16040)	addA	2889966..2893691 (+)	3726	WP_029437815.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
CUC43_RS15550 (CUC43_16045)	-	2893704..2893991 (+)	288	WP_029437816.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
CUC43_RS15555 (CUC43_16050)	gerPF	2894097..2894312 (-)	216	WP_001141566.1	spore germination protein GerPF	-
CUC43_RS15560 (CUC43_16055)	-	2894355..2894741 (-)	387	WP_029437817.1	spore germination protein GerPE	-
CUC43_RS15565 (CUC43_16060)	gerPD	2894757..2894951 (-)	195	WP_001102341.1	spore germination protein GerPD	-
CUC43_RS15570 (CUC43_16065)	gerPC	2894958..2895572 (-)	615	WP_029437818.1	spore germination protein GerPC	-
CUC43_RS15575 (CUC43_16070)	gerPB	2895641..2895847 (-)	207	WP_001012508.1	spore germination protein GerPB	-
CUC43_RS15580 (CUC43_16075)	gerPA	2895862..2896083 (-)	222	WP_001111187.1	spore germination protein GerPA	-
CUC43_RS15585 (CUC43_16080)	-	2896180..2896359 (-)	180	WP_029437819.1	aspartyl-phosphate phosphatase Spo0E family protein	-
CUC43_RS15590 (CUC43_16085)	-	2896607..2897506 (+)	900	WP_029437820.1	fumarylacetoacetate hydrolase family protein	-
CUC43_RS15595 (CUC43_16090)	-	2897542..2897826 (-)	285	WP_000925339.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142861.27 Da
• Isoelectric Point: 4.8691

>NTDB_id=255371 CUC43_RS15545 WP_029437815.1 2889966..2893691(+) (addA) [Bacillus thuringiensis LM1212]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLVLLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQTMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPDGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKVMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSLFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEIHLELGQGSIPDEIYGYSASWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATAHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=255371 CUC43_RS15545 WP_029437815.1 2889966..2893691(+) (addA) [Bacillus thuringiensis LM1212]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAATGGGCG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGTTCTCAGCACATTCGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCCTTTTGTTTACAAGTTATTAGAGGATACTATTATATGCTTGATGTTGATCCTCGTTTCC
GCATAGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATACGATGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCCTACTTATTAGAAGATGTGAAATTCCAACTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACAGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATTTAGTTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAGCAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAAGCCTGAAAGTTTCTTGCGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTTGTACAGCTTGTAAAAGTATTTACAGAACGTTTCCAAACGATGAAGCGAGATAAAGGAATGGTCGATTTCACGG
ATTTAGAGCATTTTTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGCGACGTGAAGCAGTCAATCTATCGTTTCCGACTAGCAG
AACCAGGATTATTCCTAGGAAAGTATAAACGCTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGCACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTACCTATCCAGATGGTGAAGATGTAGCAGCTGAGCTACTATGCA
TTCAGCAAACGGAAGAAGAGGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCGCAGCTTGAAGCTCGTCTTATG
GCGCAACGCATTAAAGTGATGGTCGATTCTGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTCCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTGAAATTACAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCACCAATCGTTGGATTAAACGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCTTATTCTTAAAAGGGGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGATTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGCCTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAGCGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTAGCTGGACTAGGTCGCCGCTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAATTTATTGATCCACGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTATTATATGTTGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCCTTATATAGAC
ATCGTGATAGTGAAATACATCTTGAATTAGGGCAAGGAAGCATTCCAGATGAAATTTATGGATATAGTGCGAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGTTGCAAAGTGAACGAAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACAGCTCATCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAATTACGTGCACCAATTAGAACCCGTCCTCGTTTTATGGAGAAAAAAGGTTTAACGTACGCAGAGCGTGG
AACTGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.778	100	0.539