Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   CUC43_RS15540 Genome accession   NZ_CP024771
Coordinates   2886454..2889969 (+) Length   1171 a.a.
NCBI ID   WP_029437814.1    Uniprot ID   -
Organism   Bacillus thuringiensis LM1212     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2881454..2894969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUC43_RS15495 (CUC43_15990) - 2881640..2882431 (-) 792 WP_029437810.1 phosphotransferase -
  CUC43_RS15500 (CUC43_15995) cspA 2882779..2882982 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  CUC43_RS15510 (CUC43_16005) - 2883319..2883537 (-) 219 WP_029437811.1 hypothetical protein -
  CUC43_RS15515 (CUC43_16010) - 2883824..2884009 (-) 186 WP_029437812.1 hypothetical protein -
  CUC43_RS15520 (CUC43_16015) - 2884292..2884873 (+) 582 WP_029437813.1 competence protein ComK -
  CUC43_RS15530 (CUC43_16025) - 2885124..2885717 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  CUC43_RS15535 (CUC43_16030) lepB 2885774..2886337 (+) 564 WP_000751904.1 signal peptidase I -
  CUC43_RS15540 (CUC43_16035) addB 2886454..2889969 (+) 3516 WP_029437814.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CUC43_RS15545 (CUC43_16040) addA 2889966..2893691 (+) 3726 WP_029437815.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CUC43_RS15550 (CUC43_16045) - 2893704..2893991 (+) 288 WP_029437816.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  CUC43_RS15555 (CUC43_16050) gerPF 2894097..2894312 (-) 216 WP_001141566.1 spore germination protein GerPF -
  CUC43_RS15560 (CUC43_16055) - 2894355..2894741 (-) 387 WP_029437817.1 spore germination protein GerPE -
  CUC43_RS15565 (CUC43_16060) gerPD 2894757..2894951 (-) 195 WP_001102341.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134252.36 Da        Isoelectric Point: 5.2500

>NTDB_id=255370 CUC43_RS15540 WP_029437814.1 2886454..2889969(+) (addB) [Bacillus thuringiensis LM1212]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGGMIAEFKRYNVTPSNVYEMWQQLDTHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMVCGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLETHYEARPNEKFHGEASVTIRTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAIVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGHAASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=255370 CUC43_RS15540 WP_029437814.1 2886454..2889969(+) (addB) [Bacillus thuringiensis LM1212]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGAGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAACAGGCGTTAATTGGCA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTATTGCAAGAAGTTGGT
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTGCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAACAAAACGGTTTCTTTGAACATCTTGGCGGTATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATACGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAGCGTGCCTTAATCGGAAAATATTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTCGAAAAGCTTCCGCAGTCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGATTTCATTCTT
TTTCACCGCAAGAGCTAGAAATTGTAAGACAGCTTATGGTTTGCGGAGCGAGGGTTACAATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACATTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCGCTAGCGCATT
TAGAAACGCACTATGAAGCACGTCCAAATGAAAAATTTCACGGGGAAGCAAGTGTAACGATTCGTACAGCTGCAAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGTAGACTTGTGGCGGACGAAGACTATCGTTACCGAGATATTGCGGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTAATGCGGACGCTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCGCTAGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCATGGATGTATCGTCGCT
ATCGTTCTCTTGACGATACGGACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAACAGGCTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGACGTTCCGAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGTGAAGAAAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTTGTTATTGGAGTAAATGAAGGAG
TGATCCCGGCAGCGCCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGACGTTCTTAGCGCTGCGGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGCAACTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTATGCTCATTTCGCGCAACACGGTTTATCTTTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGAGAGCTCTTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTATAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCACTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTTTACGTGAACA
TGCGAAATCGAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGA
ACCTTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCGCTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACATGGATGAAAAAAGGCCACGCAGCATCACCCGCTGGTG
TATTGTATTTCCACATTCATAACCCAATTGTTGAGGTGAAAGGTGATGCATCTGAAGCGGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGGAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCACGTTCAAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAGGCGGCGTGTACGTTCTGTAACTTCAAATCGGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.317

100

0.494

Multiple sequence alignment