Detailed information NTG0255176

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: CT122_RS03150
• Genome accession: NZ_CP024712
• Coordinates: 688170..689234 (+)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. actinidiae strain MAFF212063
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 683170..694234

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CT122_RS03140 (CT122_03140)	-	683925..685193 (+)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-
CT122_RS03145 (CT122_03145)	-	685519..687960 (-)	2442	WP_370445385.1	penicillin-binding protein 1A	-
CT122_RS03150 (CT122_03150)	pilM	688170..689234 (+)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
CT122_RS03155 (CT122_03155)	-	689234..689860 (+)	627	WP_020307188.1	PilN domain-containing protein	-
CT122_RS03160 (CT122_03160)	pilO	689857..690480 (+)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
CT122_RS03165 (CT122_03165)	pilP	690477..691004 (+)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
CT122_RS03170 (CT122_03170)	pilQ	691052..693157 (+)	2106	WP_003378848.1	type IV pilus secretin PilQ	Machinery gene
CT122_RS03175 (CT122_03175)	aroK	693162..693680 (+)	519	WP_003378847.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=255176 CT122_RS03150 WP_003378852.1 688170..689234(+) (pilM) [Pseudomonas syringae pv. actinidiae strain MAFF212063]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=255176 CT122_RS03150 WP_003378852.1 688170..689234(+) (pilM) [Pseudomonas syringae pv. actinidiae strain MAFF212063]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGTCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469