Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   CSW12_RS04970 Genome accession   NZ_CP024655
Coordinates   953536..957261 (+) Length   1241 a.a.
NCBI ID   WP_100909964.1    Uniprot ID   -
Organism   Bacillus cereus strain MLY1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 948536..962261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSW12_RS04955 (CSW12_04975) - 948694..949287 (+) 594 WP_071714132.1 TVP38/TMEM64 family protein -
  CSW12_RS04960 (CSW12_04980) lepB 949344..949907 (+) 564 WP_000751897.1 signal peptidase I -
  CSW12_RS04965 (CSW12_04985) addB 950024..953539 (+) 3516 WP_100909963.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CSW12_RS04970 (CSW12_04990) addA 953536..957261 (+) 3726 WP_100909964.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CSW12_RS04975 (CSW12_04995) - 957285..957563 (+) 279 WP_090975801.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  CSW12_RS04980 (CSW12_05000) gerPF 957707..957922 (-) 216 WP_001141566.1 spore germination protein GerPF -
  CSW12_RS04985 (CSW12_05005) - 957965..958351 (-) 387 WP_090975802.1 spore germination protein GerPE -
  CSW12_RS04990 (CSW12_05010) gerPD 958367..958561 (-) 195 WP_001052807.1 spore germination protein GerPD -
  CSW12_RS04995 (CSW12_05015) gerPC 958568..959182 (-) 615 WP_001070763.1 spore germination protein GerPC -
  CSW12_RS05000 (CSW12_05020) gerPB 959250..959456 (-) 207 WP_001012508.1 spore germination protein GerPB -
  CSW12_RS05005 (CSW12_05025) gerPA 959471..959692 (-) 222 WP_001111187.1 spore germination protein GerPA -
  CSW12_RS05010 (CSW12_05030) - 959789..959968 (-) 180 WP_000462857.1 aspartyl-phosphate phosphatase Spo0E family protein -
  CSW12_RS05015 (CSW12_05035) - 960215..961114 (+) 900 WP_064532885.1 fumarylacetoacetate hydrolase family protein -
  CSW12_RS05020 (CSW12_05040) - 961149..961430 (-) 282 WP_000926859.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142722.96 Da        Isoelectric Point: 4.8781

>NTDB_id=254810 CSW12_RS04970 WP_100909964.1 953536..957261(+) (addA) [Bacillus cereus strain MLY1]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADVALLGTLSSAARESWTRLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVMDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWASQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHNEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSKRKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITAETLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFQQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEG

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=254810 CSW12_RS04970 WP_100909964.1 953536..957261(+) (addA) [Bacillus cereus strain MLY1]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAGATGAAAAACCGAATC
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGCATCGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAGGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATGTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAGTCGTGGACAAGATTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAGGAAGAGTTATTTAGCCGCAAACCGGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTTACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGACACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AGCCAGGACTATTCCTAGGAAAATATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGACTACGATGCTGACGCTGAACTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCAGCAGAATTACTATGTA
TTCAGCAAACGGAAGAAGAAGTAATGGATGGAGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTAGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGATTTCGTTATTTTACTCCGCTCGATGCCGTGGGCGTCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATTGTGGGACTAAATGATGAAGACCTTGCGACGCTTCG
TGCTCATGGGAAGAAAGGGTCATTTTATGAAGTAATGAGCTCGTTCTTAAAAGGAGCACCGCTTGAAGAAGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAATTTACTTCAAGGATGGCGTGAATTTGCCCGTCAACAATCCCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCAGGAAAGCAAAGGCAGGCAAACTT
GCGCGTGCTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTGCGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCAAGAGCTCTTGGTGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCAGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGCTAATTTTAATCGGAACAGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAATGAATGGTTATTACCAGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCGGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTAAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCGATTACGGCCGAAACGCTACAAGAACAAATCGCAG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCAATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCCAACAAGCAAAACCAATTTTA
GAAGATCGATATAAAGTGCAACTTTCGTTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTCAAAGAGAAATG
TTTATATTTCTTTGATGGGAATCACGTTGTAAATATTGAAGGATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.087

100

0.533

Multiple sequence alignment