Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   CSW12_RS04965 Genome accession   NZ_CP024655
Coordinates   950024..953539 (+) Length   1171 a.a.
NCBI ID   WP_100909963.1    Uniprot ID   -
Organism   Bacillus cereus strain MLY1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 945024..958539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSW12_RS04925 (CSW12_04945) - 945190..945684 (-) 495 WP_000182394.1 DUF6584 family protein -
  CSW12_RS04930 (CSW12_04950) cspA 945969..946172 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  CSW12_RS04935 (CSW12_04955) - 946369..946572 (-) 204 WP_090975793.1 hypothetical protein -
  CSW12_RS04940 (CSW12_04960) - 946921..947106 (-) 186 WP_090975795.1 hypothetical protein -
  CSW12_RS04945 (CSW12_04965) - 947389..947970 (+) 582 WP_071714131.1 competence protein ComK -
  CSW12_RS04950 (CSW12_04970) - 948042..948341 (-) 300 WP_080476084.1 hypothetical protein -
  CSW12_RS04955 (CSW12_04975) - 948694..949287 (+) 594 WP_071714132.1 TVP38/TMEM64 family protein -
  CSW12_RS04960 (CSW12_04980) lepB 949344..949907 (+) 564 WP_000751897.1 signal peptidase I -
  CSW12_RS04965 (CSW12_04985) addB 950024..953539 (+) 3516 WP_100909963.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CSW12_RS04970 (CSW12_04990) addA 953536..957261 (+) 3726 WP_100909964.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CSW12_RS04975 (CSW12_04995) - 957285..957563 (+) 279 WP_090975801.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  CSW12_RS04980 (CSW12_05000) gerPF 957707..957922 (-) 216 WP_001141566.1 spore germination protein GerPF -
  CSW12_RS04985 (CSW12_05005) - 957965..958351 (-) 387 WP_090975802.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134037.20 Da        Isoelectric Point: 5.1424

>NTDB_id=254809 CSW12_RS04965 WP_100909963.1 950024..953539(+) (addB) [Bacillus cereus strain MLY1]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGAKVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVADEKYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTAEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEILGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVRATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKTGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRSLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=254809 CSW12_RS04965 WP_100909963.1 950024..953539(+) (addB) [Bacillus cereus strain MLY1]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATCGCGGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGTTACTATACGATGATTTTGAACGTGCTTTAATTGGAAAATACTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAAAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGTGTTGCTCGTGAAATTCGTAAACTTGTTGCGGACGAAAAATATCGTTACCGAGATATTGCAGT
GCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTACAATATCCCGCACTTCATCGATG
AAAAGCGCCCGATGTCACACCACCCATTGGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGTTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAGCGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTGCTGAAGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGCTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTGTTTTTAGAAGAACTGGATGTTCCGAAGAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACATTTGTAGAGATTCTTGGGGAAGAGAAA
ATGTCTCTTTCTATGTTTACTGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGGTTATCTGATGTGAGAGCGACGTTTATTATAGGTGTAAATGAGGGTG
TCATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCAGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGTTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCGTTTATTACGAATGATGTAAACGACTTATCACGCTCAGAACAAATTTCATATGTAGCAACGCCA
GAAGTGACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAACGATACGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAATTTCTACGTAACGTCAGATGAATGGAAACAAAAAAGTAGTCGCGTGCTATCAAGTTTATTTTACCGAA
ATCGTGCGCAGAAACTAAGTACAGCAGTAAGTAGAGATTTATACGGAGATACAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAATCGTTGTGCATACGCTCATTTCGCGCAGCATGGTTTGTCGCTTAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGCACTTGGGCGG
ATTTATCAATAAAAGAATGTGAACATCTTTCTGTTTTAGTAATAGAAGAAATTGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATTATTTTCCGCACGTCAATCATTCTTCGTGAACA
TGCGAAGTCGAGCGGATTCGTACCAGTTGATTTAGAGGTGCCGTTCGGTATGGGTGGTACAGGGTCACTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTAAAGATGGAAGTAGTCGGTCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCACAGACGTGGATGAAAACAGGCCACGCAGCATCACCAGCTGGTG
TATTGTATTTCCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCAGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATTGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAGTTCCGATCGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.232

100

0.493

Multiple sequence alignment