Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   CS376_RS19420 Genome accession   NZ_CP024203
Coordinates   3836542..3837687 (+) Length   381 a.a.
NCBI ID   WP_015240777.1    Uniprot ID   -
Organism   Bacillus velezensis strain NKG-1     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3831542..3842687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS376_RS19395 - 3832441..3832869 (-) 429 WP_007407634.1 DUF1934 domain-containing protein -
  CS376_RS19400 - 3833023..3833528 (+) 506 Protein_3758 bacteriocin biosynthesis protein AlbG -
  CS376_RS19405 - 3833540..3834916 (-) 1377 WP_099567168.1 YncE family protein -
  CS376_RS19410 - 3834956..3836311 (-) 1356 WP_015240776.1 YncE family protein -
  CS376_RS19420 rapF 3836542..3837687 (+) 1146 WP_015240777.1 tetratricopeptide repeat protein Regulator
  CS376_RS19425 phrF 3837671..3837790 (+) 120 WP_015240778.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  CS376_RS19430 - 3837988..3838191 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  CS376_RS19435 - 3838205..3838408 (+) 204 WP_099567169.1 hypothetical protein -
  CS376_RS19440 - 3838443..3838628 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  CS376_RS19445 - 3838814..3839095 (+) 282 WP_007407643.1 hypothetical protein -
  CS376_RS19450 - 3839139..3839651 (+) 513 WP_099567170.1 stage II sporulation protein M -
  CS376_RS19460 - 3840242..3840460 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  CS376_RS19465 speB 3840628..3841500 (-) 873 WP_007614448.1 agmatinase -
  CS376_RS19470 speE 3841559..3842389 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45572.57 Da        Isoelectric Point: 4.5155

>NTDB_id=252937 CS376_RS19420 WP_015240777.1 3836542..3837687(+) (rapF) [Bacillus velezensis strain NKG-1]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYEIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=252937 CS376_RS19420 WP_015240777.1 3836542..3837687(+) (rapF) [Bacillus velezensis strain NKG-1]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACATATTTTTCT
ATGGATTATGCCCGGCAGGCTTATGAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTGGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(190-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57

Multiple sequence alignment