Detailed information NTG0025218

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BTH_RS14560
• Genome accession: NC_007651
• Coordinates: 521485..522786 (-)
• Length: 433 a.a.
• NCBI ID: WP_009893082.1
• Uniprot ID: Q2T1C5
• Organism: Burkholderia thailandensis E264
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 516485..527786

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BTH_RS14545 (BTH_I0463)	nirD	517375..517770 (-)	396	WP_009893098.1	nitrite reductase small subunit NirD	-
BTH_RS14550 (BTH_I0464)	nirB	517846..520290 (-)	2445	WP_009893095.1	nitrite reductase large subunit NirB	-
BTH_RS14555 (BTH_I0465)	maiA	520683..521327 (+)	645	WP_009893084.1	maleylacetoacetate isomerase	-
BTH_RS14560 (BTH_I0466)	pilA	521485..522786 (-)	1302	WP_009893082.1	signal recognition particle-docking protein FtsY	Machinery gene
BTH_RS14570 (BTH_I0468)	rsmD	523044..523670 (+)	627	WP_019255510.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BTH_RS14575 (BTH_I0469)	coaD	523779..524279 (+)	501	WP_004195249.1	pantetheine-phosphate adenylyltransferase	-
BTH_RS14580 (BTH_I0470)	-	524344..524607 (+)	264	WP_009893077.1	YfhL family 4Fe-4S dicluster ferredoxin	-
BTH_RS14585 (BTH_I0471)	hisC	524741..525808 (-)	1068	WP_011401783.1	histidinol-phosphate transaminase	-
BTH_RS14590 (BTH_I0472)	pth	525882..526487 (-)	606	WP_009893073.1	aminoacyl-tRNA hydrolase	-
BTH_RS14595 (BTH_I0473)	-	526601..527215 (-)	615	WP_025369612.1	50S ribosomal protein L25/general stress protein Ctc	-

Sequence

Protein

• Length: 433 a.a.
• Molecular weight: 45283.62 Da
• Isoelectric Point: 5.3487

>NTDB_id=25218 BTH_RS14560 WP_009893082.1 521485..522786(-) (pilA) [Burkholderia thailandensis E264]
MFSFFKRFKKAPDAAPAEPQQAPDAPQDAAGESAAPEAASKAARVDAPAAPVSRGEYSEAPATERPRSDAPAPVRATPPV
SPAAATPESPAGQPSAAEAARAPSVVMTVTPAANGRNGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSITNVFVN
TKIDDALYEELETALLMSDAGVDATEHLLGALREKVRTGRLTDPQQVKDALRGLLVELLTPLEQSLVLGRAQPLVMMIAG
VNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARNIDVMMA
DTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGILAAIA
RQRPIPVYFIGVGEKVEDLQPFSAEEFADALLG

Nucleotide

• Length: 1302 bp

>NTDB_id=25218 BTH_RS14560 WP_009893082.1 521485..522786(-) (pilA) [Burkholderia thailandensis E264]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAGCGCCCGACGCCGCACCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCA
GGACGCGGCCGGCGAGTCGGCCGCGCCCGAGGCCGCGTCCAAGGCCGCGCGAGTCGACGCGCCCGCTGCGCCGGTCTCGC
GCGGCGAGTACAGCGAAGCGCCGGCTACCGAACGGCCCCGTTCCGACGCGCCGGCCCCCGTTCGCGCGACACCGCCCGTC
TCCCCCGCAGCAGCGACGCCGGAATCGCCGGCCGGGCAGCCGTCCGCCGCCGAAGCGGCGCGCGCGCCGTCCGTCGTGAT
GACGGTGACGCCGGCCGCCAACGGCCGCAACGGCGTCGTCGAGACGGTCGAGATCGTGCCGCCGCCCGCGCCCGAGCCGG
CCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATCACGAACGTGTTCGTGAAC
ACGAAGATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGGCGTCGACGCGACCGAGCA
TCTGCTCGGCGCGCTGCGCGAGAAGGTGCGCACGGGCCGCCTCACCGATCCGCAACAGGTGAAGGACGCGCTGCGCGGCC
TGCTCGTCGAGTTGCTGACGCCGCTCGAGCAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTCGTGATGATGATCGCGGGC
GTGAACGGCGCGGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGATCAATCGGTGCTGCTCGC
CGCCGGCGACACGTTCCGCGCGGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAGCGCAACAACGTGACCGTCGTCCAGC
AGGAAAGCGGCGATCCGGCCGCGGTGATTTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAACATCGACGTGATGATGGCC
GACACCGCGGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCGCGTGATCGGCAAGGCGCA
CGACGGCGCGCCGCACGAAGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCGCGCAGGTGAAGGCCTTCG
ACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGCATCCTCGCCGCGATCGCG
CGCCAGCGGCCGATTCCGGTGTACTTCATCGGCGTCGGCGAGAAGGTCGAGGATCTGCAGCCGTTCAGCGCGGAGGAATT
CGCGGACGCGCTGCTCGGATAA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (152-212)

• SRP54-type protein, GTPase domain (SRP54) (234-433)

3D structure

• AlphaFold DB: Q2T1C5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	50.227	100	0.51