Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   CG479_RS04930 Genome accession   NZ_CP024109
Coordinates   971148..974873 (+) Length   1241 a.a.
NCBI ID   WP_041810256.1    Uniprot ID   -
Organism   Bacillus cytotoxicus strain CH_13     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 966148..979873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG479_RS04915 (CG479_004915) - 966292..966885 (+) 594 WP_011983946.1 TVP38/TMEM64 family protein -
  CG479_RS04920 (CG479_004920) lepB 966946..967512 (+) 567 WP_011983947.1 signal peptidase I -
  CG479_RS04925 (CG479_004925) addB 967636..971148 (+) 3513 WP_011983948.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CG479_RS04930 (CG479_004930) addA 971148..974873 (+) 3726 WP_041810256.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CG479_RS04935 (CG479_004935) - 974967..975182 (-) 216 WP_011983950.1 spore germination protein -
  CG479_RS04940 (CG479_004940) - 975247..975615 (-) 369 WP_011983951.1 spore germination protein GerPE -
  CG479_RS04945 (CG479_004945) - 975630..975824 (-) 195 WP_011983952.1 hypothetical protein -
  CG479_RS04950 (CG479_004950) - 975829..976449 (-) 621 WP_011983953.1 spore germination protein GerPC -
  CG479_RS04955 (CG479_004955) - 976510..976716 (-) 207 WP_011983954.1 spore germination protein GerPB -
  CG479_RS04960 (CG479_004960) - 976734..976955 (-) 222 WP_011983955.1 spore germination protein -
  CG479_RS04965 (CG479_004965) - 977082..977261 (-) 180 WP_011983956.1 aspartyl-phosphate phosphatase Spo0E family protein -
  CG479_RS04970 (CG479_004970) - 977508..978407 (+) 900 WP_081428427.1 fumarylacetoacetate hydrolase family protein -
  CG479_RS04975 (CG479_004975) - 978516..979706 (-) 1191 WP_011983958.1 ornithine--oxo-acid transaminase -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 143786.38 Da        Isoelectric Point: 5.1012

>NTDB_id=252051 CG479_RS04930 WP_041810256.1 971148..974873(+) (addA) [Bacillus cytotoxicus strain CH_13]
MVENWPAKPEGSQWTDDQWKAVVAHGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIEEPSSRHIRKQLSLLNKASISTIHSFCLQVIRSYYYMLDIDPRFRIANQTENELLKEEVLDDILEEEYGME
ENQLFFELVDRYTSDRNDDDLQRMILALHTAAGAHPNPEKWLDRLVEAYNVEGKTIEDLMYASYLLEDVKFQLETATEHI
RKAMELAMLPDGPAPRMETLQTDLVLLETLSHAARKSWTSVYEAMQHVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQEMHPVLGKLVQLVKEFSNRFQAIKRDKGMVDFTDLEHFCLQILSEQGEDGELRPSPVALQ
YRNRFAEVLVDEYQDTNFVQESIIKLVTKDSEQEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLEGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIEYDADAELKLGASYPEGEDVAAELLCIHQSEEEVLDGEEGEEVEKAQLEARLI
AQRIKAMVDSGYTVYDRKTNEMRQVQYRDFVILLRSMPWAPQIMEELKLQGIPVYAELATGYFEATEVNIMMNVFRVIDN
PVQDIPLAAVLRSPIVGLNDEELAMLRAHAKKGSFYEVMRSFLRGAPLEGGKELHEKLKWFYHLLQGWREFARQQSLSDL
IWKVYRETGYYDFVGGLPGGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMQRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKRELIAEEMRVLYVALTR
AKEKLILIGTVKDKEKEMEKWLDTREHTEWLLPDYVRASASCYLDWIAPSLYRHRDSEILLELGQGTIPNEIYEYDTSWK
VEFVDGKTLLAPEPAQEEKQELLEALREKKAVPLESERKDEVYNRLTWKYEYEDATLQRAKQSVTEIKRNYQSEDGSDTA
FIQKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKQSITIESIQEQIAKMVNKEILTFEQAEEISVERIVAFFES
HLGKRVLEAKSVEREVPFTMMLSAKEAYQNWQGKSEETILVQGVIDCMIEEDDGITLIDFKTDTIEGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKTLQHPVKEKCLYFFDGNHVITIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=252051 CG479_RS04930 WP_041810256.1 971148..974873(+) (addA) [Bacillus cytotoxicus strain CH_13]
ATGGTAGAAAATTGGCCAGCAAAACCAGAAGGGAGTCAATGGACAGATGATCAATGGAAAGCTGTTGTGGCACATGGACG
TGATATTTTAGTTGCAGCAGCAGCAGGTTCCGGGAAAACAGCAGTACTCGTTGAACGTATCATTAAAAAGATTATTAATG
AGGAGAATCCGGTAGATGTCGATCGCTTACTCGTTGTAACATTTACGAATGCAGCCGCGCAAGAGATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGAGGAACCGAGTTCACGGCATATACGGAAGCAGCTGAGCTTATTAAATAAGGC
TTCCATCTCAACAATTCATTCTTTTTGTTTACAAGTCATTCGTTCATATTATTACATGCTTGATATCGATCCTCGTTTTC
GCATCGCGAATCAAACGGAAAATGAGTTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATGGAA
GAGAATCAACTATTCTTTGAACTTGTCGATCGCTATACGAGCGACCGTAATGATGATGATTTGCAGCGAATGATTTTGGC
GCTGCATACAGCAGCAGGAGCGCATCCAAATCCAGAAAAGTGGCTTGATAGATTAGTAGAAGCGTATAATGTGGAAGGAA
AAACAATTGAAGATTTAATGTATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAACGGAGCACATT
CGAAAAGCAATGGAATTAGCGATGCTTCCCGATGGTCCTGCTCCTCGCATGGAGACATTACAAACGGATCTCGTTTTACT
AGAAACGCTATCTCATGCTGCGCGCAAGTCGTGGACAAGTGTATATGAAGCAATGCAGCATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGCGATTATAACGAAGATATTGTGAAACAAGTCGATTCTCTTCGCAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAACTGTTTAGTCGTAAACCAGAAAGCTTTTTACGTGATTTTCAAGAGATGCACCCTGTATTAGG
AAAACTCGTGCAGCTTGTTAAAGAGTTTTCGAACCGATTTCAAGCGATAAAACGTGATAAAGGAATGGTTGATTTTACAG
ATTTAGAGCATTTTTGTTTGCAAATTTTAAGCGAGCAAGGTGAAGATGGTGAACTGCGCCCATCGCCAGTTGCACTTCAA
TACCGCAATAGATTTGCGGAAGTTCTCGTTGATGAATATCAAGATACAAACTTCGTTCAAGAATCCATTATTAAACTCGT
AACGAAAGACTCTGAGCAAGAAGGTAATTTGTTTATGGTCGGTGACGTAAAACAGTCTATTTATCGCTTTCGATTAGCAG
AACCCGGCTTGTTTTTAGGAAAATATAAACGTTTTACACAAGAAGGATTAGAAGGCGGAATGAAAATTGATTTAGCGAAA
AACTTCCGCAGTCGTCATGAAGTATTAGCTGGTACAAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGTGAAAT
TGAGTATGATGCTGACGCTGAATTAAAGTTAGGTGCAAGCTATCCAGAAGGAGAAGATGTAGCGGCAGAGTTATTATGCA
TTCACCAATCTGAAGAAGAAGTGCTAGATGGAGAAGAGGGTGAAGAAGTAGAAAAAGCGCAGCTGGAAGCACGTCTCATT
GCGCAGCGCATAAAAGCGATGGTTGACTCAGGATATACAGTATATGACCGGAAAACGAATGAAATGCGTCAGGTCCAATA
TCGCGATTTTGTTATTTTACTGCGCTCGATGCCGTGGGCGCCGCAAATTATGGAAGAATTAAAGCTGCAAGGCATTCCAG
TATACGCAGAACTTGCAACGGGCTATTTTGAAGCGACAGAAGTAAATATTATGATGAATGTTTTCCGCGTTATTGATAAT
CCGGTGCAAGATATTCCGCTAGCAGCTGTACTTCGTTCGCCAATTGTTGGGCTAAATGATGAGGAACTTGCGATGCTTCG
TGCGCATGCAAAGAAAGGCTCTTTTTATGAGGTGATGCGCTCGTTCTTACGAGGAGCACCGCTTGAAGGAGGGAAGGAAC
TGCATGAAAAGCTAAAGTGGTTTTATCACTTACTGCAAGGATGGCGTGAGTTTGCGCGTCAGCAATCTCTTTCTGACTTA
ATTTGGAAAGTGTATCGTGAAACTGGGTATTATGACTTTGTCGGCGGATTACCTGGCGGAAAGCAACGTCAGGCAAACTT
GCGTGTTTTATACGACCGCGCAAGGCAATATGAGGCGACATCATTTAGAGGGTTATTCCGCTTTTTACGCTTTATTGAGC
GCATTTTAGAACGCGGTGATGATATGGGAACTGCAAGAGCTCTTGGAGAGCAAGAAGATGTTGTGCGCATTATGACCATT
CATAAAAGTAAAGGACTAGAGTTCCCAGTTGTGTTTGTGGCAGGGCTCGGCCGTCGTTTTAATACGCAAGATTTAATGCA
GCGTTTCTTATTGCATAAAGACTTCGGATTTGGTTCACAATTTATTGACCCGCGCAAGCGAATTAAATATACGACATTAT
CGCAATTAGCGATTAAACGAAAAATGAAACGCGAGTTAATTGCAGAAGAAATGCGTGTATTATATGTTGCGTTAACGCGT
GCAAAAGAAAAATTAATTTTAATTGGAACGGTGAAAGATAAGGAAAAAGAAATGGAAAAATGGCTTGATACACGGGAGCA
TACAGAATGGTTACTACCAGATTATGTACGCGCTAGTGCGTCATGTTATTTAGATTGGATTGCTCCTTCCTTATACCGAC
ATCGAGATAGTGAAATACTCCTTGAATTAGGACAAGGTACGATTCCAAATGAAATTTATGAATATGATACAAGCTGGAAA
GTAGAATTCGTTGATGGCAAAACGTTACTCGCACCAGAGCCAGCTCAAGAAGAAAAGCAAGAGTTGTTAGAAGCGCTGCG
TGAGAAAAAGGCTGTCCCGTTAGAGAGTGAACGAAAAGATGAAGTATATAATCGATTAACATGGAAATACGAATACGAGG
ATGCAACGCTTCAACGTGCGAAGCAATCTGTTACAGAGATTAAAAGAAATTATCAATCGGAAGATGGAAGTGATACGGCC
TTTATTCAAAAACTACGTGCGCCAATTCGAACACGTCCGCGTTTTATGGAGAAAAAAGGCTTAACATATGCAGAGCGTGG
AACAGCTGTTCATGCCGTTATGCAGCATGTTGATTTGAAGCAATCTATTACAATTGAATCTATTCAAGAGCAAATCGCAA
AAATGGTGAATAAAGAGATCTTAACCTTTGAACAAGCAGAAGAAATCAGTGTCGAAAGAATAGTGGCATTTTTTGAAAGT
CATCTTGGAAAACGAGTGTTAGAAGCGAAAAGTGTTGAGCGAGAAGTACCGTTTACGATGATGCTTTCAGCAAAAGAAGC
GTATCAAAATTGGCAAGGTAAGAGCGAAGAGACGATACTCGTACAAGGGGTTATCGACTGTATGATCGAAGAAGATGATG
GCATTACGTTAATTGACTTTAAAACAGATACAATTGAAGGGAAATTTCCAGGTGGATTCGATCAGGCGAAACCGATTTTA
GAAGAGCGCTATAAAGTACAGCTTTCCTTATATGCTAAAGCACTAGAAAAAACGTTACAACACCCTGTAAAAGAAAAGTG
TTTATACTTTTTTGATGGAAATCATGTGATTACGATTGAGGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1058-1231)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.408

100

0.537

Multiple sequence alignment