Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   CG479_RS04925 Genome accession   NZ_CP024109
Coordinates   967636..971148 (+) Length   1170 a.a.
NCBI ID   WP_011983948.1    Uniprot ID   A7GM36
Organism   Bacillus cytotoxicus strain CH_13     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 962636..976148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG479_RS04900 (CG479_004900) aceA 963220..964497 (+) 1278 WP_011983943.1 isocitrate lyase -
  CG479_RS04905 (CG479_004905) - 964828..965031 (+) 204 WP_011983944.1 cold-shock protein -
  CG479_RS04910 (CG479_004910) - 965429..966019 (+) 591 WP_011983945.1 competence protein ComK -
  CG479_RS22890 - 966098..966226 (-) 129 WP_255815495.1 hypothetical protein -
  CG479_RS04915 (CG479_004915) - 966292..966885 (+) 594 WP_011983946.1 TVP38/TMEM64 family protein -
  CG479_RS04920 (CG479_004920) lepB 966946..967512 (+) 567 WP_011983947.1 signal peptidase I -
  CG479_RS04925 (CG479_004925) addB 967636..971148 (+) 3513 WP_011983948.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CG479_RS04930 (CG479_004930) addA 971148..974873 (+) 3726 WP_041810256.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CG479_RS04935 (CG479_004935) - 974967..975182 (-) 216 WP_011983950.1 spore germination protein -
  CG479_RS04940 (CG479_004940) - 975247..975615 (-) 369 WP_011983951.1 spore germination protein GerPE -
  CG479_RS04945 (CG479_004945) - 975630..975824 (-) 195 WP_011983952.1 hypothetical protein -

Sequence

Protein


Download         Length: 1170 a.a.        Molecular weight: 134521.03 Da        Isoelectric Point: 5.3403

>NTDB_id=252050 CG479_RS04925 WP_011983948.1 967636..971148(+) (addB) [Bacillus cytotoxicus strain CH_13]
MSLRFVIGRAGSGKSTLCLREVQEELKERPRGKTILYLVPEQMTFQTQQALIGSEEVRASIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIIESRKEGLYVFQKAAEQNGFFEHLGGMIAEFKRYNITPSHVYEMWQQLDAHSSSAEQKLLA
NKIYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLPFSEYVKDAEIYIDGFHSFSPQELEIIRQLMRCGARMTITLTVDE
KTFAQPVNELDLFYETVLTYEKVKQVAREEKVVMEKTISLVEQPRFHSPALAHLEAHYESRPNEKFYGETSIAIHTAANL
RAEVEGVAREIRRLVAEENYRYRDIAVLLRNGESYYDVLRTLFADYDIPHFIDEKRSMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVKKEVMREEMDEFENYCLAYGVQGKKWTADEPWTYRRYRSLDEGNEVITDRERQMEEKMNRLRE
IVRTPIIRLQKRLKRASTVMQMCEAVYLFLEELDVPKKLEQLRVRAEESGDFLFATDHDQVWEEVMNLLDTFVEMLGEES
MSLSVFIDVMTTGLEALQFANIPPSLDQVLIANIDRSRLSNIRATFVIGANEGVMPATPTDEGMLSDEERDVLAAAGVEL
APTTRQTLLEEQFVLYQMVTRASEKLYISCPLADEEGKTLLASSFMKKIKRMFPNVKETFVTNDVNDLSRAAQISYVTTP
EVTLSYVMQQLQTWKRYGFEGDLDFWWDVYNFYVTSDEWKQKSNRVLSSLFYRNRAKKLSTAVSRILYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLREKRTWADLSIKECEHLSILVIEEIAPLLQRQIL
LSSNRHAYLKQKLQQIIFRTSLVLREHAKASGFVPVDLEVPFGMGGKDSLPPMEFTLSNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVISNAGTWMKQGEVASPAGVLYFHIHNPLVEVKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSNIASEQEFSVLQKYVHHTFETIGKDITEGVIDIAP
YKMGNKAACTFCHFRSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGE

Nucleotide


Download         Length: 3513 bp        

>NTDB_id=252050 CG479_RS04925 WP_011983948.1 967636..971148(+) (addB) [Bacillus cytotoxicus strain CH_13]
ATGTCACTTCGATTTGTGATTGGAAGAGCTGGAAGTGGGAAAAGTACACTTTGTTTACGAGAAGTGCAAGAAGAGTTAAA
AGAGCGCCCAAGAGGGAAAACAATCTTGTATCTTGTTCCTGAACAAATGACATTTCAGACGCAGCAGGCGTTAATAGGAA
GTGAAGAGGTAAGAGCTTCTATTCGTGCACAAGTGTTTAGTTTTTCACGATTAGCTTGGAAAGTGTTGCAAGAAGTAGGA
GGGGCAAGTCGTCTTCATATTGATGAAGCGGGTGTGCATATGTTACTTCGCAAAATTATTGAATCGCGCAAAGAAGGATT
ATATGTGTTTCAAAAAGCCGCTGAACAAAATGGCTTCTTTGAACATCTTGGAGGTATGATTGCAGAGTTTAAACGTTACA
ATATAACGCCATCTCATGTATATGAAATGTGGCAACAGTTAGATGCGCATAGCAGTAGCGCCGAACAAAAGTTGCTGGCA
AATAAAATATATGATTTGCAGCTATTATATGATGATTTTGAACGAGCTTTAATTGGAAAGTATTTAGATTCGGAAGATTA
TTTACAGCTCCTTATTGAGAAACTGCCGTTTTCTGAGTATGTAAAGGATGCAGAAATTTATATTGATGGATTTCATTCGT
TCTCCCCGCAAGAGCTTGAAATTATAAGGCAGCTAATGAGATGCGGAGCAAGAATGACGATTACATTAACGGTGGATGAA
AAAACGTTCGCCCAGCCAGTCAATGAACTGGATTTGTTTTATGAAACAGTGCTGACGTATGAAAAAGTAAAACAAGTAGC
ACGTGAAGAGAAAGTGGTAATGGAAAAAACGATTTCGCTCGTAGAACAGCCGCGATTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCACTATGAATCGCGTCCGAACGAAAAGTTTTACGGAGAGACGAGTATTGCCATCCATACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGAGTAGCGCGAGAAATTCGTAGACTTGTTGCAGAAGAGAATTATCGATACCGCGATATTGCCGT
ACTTCTTCGGAACGGAGAAAGCTATTATGATGTGTTAAGAACATTATTTGCAGATTATGATATTCCGCATTTCATTGATG
AAAAGCGCTCGATGTCTCACCATCCGTTAGTCGAATGTATTCGTTCTGCTTTAGAAATAATTAGTGGGAATTGGCGTTAT
GATGCTGTTTTTCGCTGCGTGAAAACAGAACTTTTATATCCGCTGGATGTAAAAAAAGAAGTGATGCGTGAAGAAATGGA
TGAGTTTGAAAACTACTGTTTGGCGTATGGTGTCCAAGGTAAGAAATGGACCGCCGATGAGCCGTGGACGTATCGTCGTT
ATCGCTCTCTTGATGAGGGGAACGAGGTGATAACAGATCGTGAACGCCAAATGGAAGAGAAAATGAACCGTCTTCGTGAG
ATTGTAAGAACGCCAATCATTCGTCTGCAAAAAAGATTGAAACGTGCAAGTACTGTTATGCAAATGTGCGAAGCTGTATA
TTTATTTTTAGAGGAGCTAGATGTTCCGAAGAAATTAGAACAACTACGTGTACGTGCTGAAGAAAGCGGGGATTTCTTAT
TTGCGACAGACCATGATCAAGTGTGGGAAGAAGTGATGAATCTTCTTGATACATTTGTTGAGATGCTTGGAGAAGAAAGT
ATGTCTCTATCGGTGTTTATTGATGTGATGACGACAGGGCTTGAGGCACTTCAATTTGCCAATATCCCGCCATCGTTAGA
CCAAGTATTAATTGCAAATATCGATCGCTCTAGACTTTCAAATATTCGGGCAACATTTGTGATTGGTGCAAATGAAGGTG
TGATGCCAGCTACGCCGACAGATGAAGGGATGCTTTCTGATGAAGAAAGAGATGTTCTTGCAGCAGCGGGCGTGGAATTA
GCACCAACGACAAGACAAACGTTATTAGAAGAACAGTTTGTCTTGTATCAAATGGTGACAAGAGCATCTGAGAAACTATA
TATCTCTTGTCCTCTTGCAGATGAAGAAGGAAAGACATTACTAGCTTCTAGTTTTATGAAGAAAATAAAGAGAATGTTTC
CTAATGTGAAAGAGACGTTTGTAACAAACGATGTAAACGACTTATCGCGCGCAGCACAAATTTCATATGTAACGACGCCA
GAAGTAACATTGTCATATGTGATGCAACAACTGCAAACGTGGAAGCGATACGGTTTTGAAGGTGATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTTACATCCGATGAATGGAAGCAAAAAAGCAACCGTGTATTATCCAGTTTGTTTTATCGAA
ATCGTGCGAAGAAATTAAGTACGGCTGTAAGTCGAATTTTATATGGAGATAAAATAAAAGGAAGTGTCTCTCGTATGGAA
TTATTTAATCGCTGTGCGTACGCTCATTTTGCACAGCACGGTTTATCATTGAGAGAGCGCGATATATTTAAATTAGATGC
ACCAGATATCGGGGAGCTGTTCCATGCAGCGCTAAAGAAAATTGCGGACAAGCTCTTGCGTGAAAAACGGACTTGGGCTG
ATTTATCCATAAAAGAATGTGAGCATCTTTCCATATTAGTAATAGAAGAAATTGCTCCATTATTACAAAGGCAAATTTTA
TTAAGCTCAAATCGACATGCTTATTTGAAACAAAAATTACAGCAAATTATTTTTAGAACATCGCTTGTGCTTAGGGAACA
TGCTAAGGCGAGTGGATTTGTTCCAGTTGATTTAGAAGTTCCGTTTGGTATGGGAGGCAAAGACTCATTACCACCGATGG
AATTTACATTATCAAACGGTGTGAAAATGGAAGTTGTCGGCCGGATTGATCGAGTTGATAAAGCGGAAGATGAGAGCGGT
ACGTTTCTTCGCATTATTGATTATAAATCGAGCTCGAAAGCGCTGGATTTAACAGAAGTATATTACGGATTAGCATTGCA
AATGCTGACGTATTTGGATGTCGTCATTTCTAATGCTGGTACGTGGATGAAACAGGGGGAAGTCGCATCACCAGCAGGTG
TGTTATACTTCCATATTCATAATCCACTTGTTGAGGTGAAAGGTGACGCTTCTGAAGAGGAGATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTGCTTGGAGATGCAGATGTTGTTCGTTTAATGGATAACAAACTATCAACAGGAAG
CTCTGACATTATTTCAGCCGGGTTAAAAAAAGACGGAAGCTTCAGCGCACGCTCTAACATCGCAAGTGAACAAGAATTTT
CTGTGCTACAAAAATACGTACACCATACGTTTGAAACGATCGGAAAAGACATTACAGAAGGTGTCATTGATATTGCTCCA
TATAAAATGGGGAATAAAGCAGCGTGCACATTTTGTCACTTCCGCTCTGTCTGCCAGTTCGATGAATCGCTGGAAGACAA
TCAGTTCCGTACATTAAAAGATATGAAAGATAGTGAAGCAATGGAGAAAATACGAGAGGAGGTCGGGGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(308-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GM36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.616

100

0.497

Multiple sequence alignment