Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMUL_RS00445 Genome accession   NC_007614
Coordinates   87281..88747 (-) Length   488 a.a.
NCBI ID   WP_011379448.1    Uniprot ID   -
Organism   Nitrosospira multiformis ATCC 25196     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 82281..93747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMUL_RS00415 (Nmul_A0078) mutY 82322..83473 (+) 1152 WP_011379442.1 A/G-specific adenine glycosylase -
  NMUL_RS00420 (Nmul_A0079) - 83497..84150 (+) 654 WP_011379443.1 5-formyltetrahydrofolate cyclo-ligase -
  NMUL_RS00425 (Nmul_A0080) - 84182..84817 (-) 636 WP_011379444.1 flavin prenyltransferase UbiX -
  NMUL_RS00430 (Nmul_A0081) rapZ 84905..85744 (-) 840 WP_011379445.1 RNase adapter RapZ -
  NMUL_RS00435 (Nmul_A0082) hprK 85831..86775 (-) 945 WP_011379446.1 HPr(Ser) kinase/phosphatase -
  NMUL_RS00440 (Nmul_A0083) hpf 86952..87254 (-) 303 WP_011379447.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NMUL_RS00445 (Nmul_A0084) rpoN/rpoN1 87281..88747 (-) 1467 WP_011379448.1 RNA polymerase factor sigma-54 Machinery gene
  NMUL_RS00450 (Nmul_A0085) lptB 88798..89604 (-) 807 WP_011379449.1 LPS export ABC transporter ATP-binding protein -
  NMUL_RS00455 (Nmul_A0086) lptA 89617..90189 (-) 573 WP_011379450.1 lipopolysaccharide transport periplasmic protein LptA -
  NMUL_RS00460 (Nmul_A0087) lptC 90173..90745 (-) 573 WP_011379451.1 LPS export ABC transporter periplasmic protein LptC -
  NMUL_RS00465 (Nmul_A0088) - 90742..91266 (-) 525 WP_011379452.1 KdsC family phosphatase -
  NMUL_RS00470 (Nmul_A0089) ispH 91488..92432 (-) 945 WP_041352697.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  NMUL_RS00475 (Nmul_A0090) - 92720..93715 (-) 996 WP_011379454.1 cobalamin-binding protein -

Sequence

Protein


Download         Length: 488 a.a.        Molecular weight: 55010.70 Da        Isoelectric Point: 4.8352

>NTDB_id=25108 NMUL_RS00445 WP_011379448.1 87281..88747(-) (rpoN/rpoN1) [Nitrosospira multiformis ATCC 25196]
MKPTLQVKLSQHLALTPQLQQSIRLLQLSTIELNQEIERIVQENPLLELDDSLGNDSTDYHSLIPEELAAPLPNELEVAE
ESLPILSVEAETDVKQPLDEREWPPDDSVFRPYRDDEDERDIPQQAVEPPNLRAHLNSQLSLSQISQRDRKITGLLIDSL
DDDGYLVQDLEELVDLLPAELSIDIDDLHIALEHLQHLDPPGIGARNLRECLVMQLQALPPDTPYLEQALALVNNHLESL
ASRDFGAIKRVLHCNDDCLRSVQQMITRLNPRPATAFSSTVACYIVPDVIVTKVGGSWVASLNPEAMPRLRINRLYAEIL
KGCNDDSTRRLISQLQEAKWLVKNVQQRFNTILKASAAIVERQQQFFEHGAVAMRPMILREIADVLNLHESTISRVTTQK
FMRTPRGIFELKYFFGSHVSTDSGGACSATAIRALIKQMISEENSRKPLSDSQISEVLGQQGIMVARRTVAKYRESLQIP
SVNLRKSF

Nucleotide


Download         Length: 1467 bp        

>NTDB_id=25108 NMUL_RS00445 WP_011379448.1 87281..88747(-) (rpoN/rpoN1) [Nitrosospira multiformis ATCC 25196]
ATGAAACCAACTCTTCAAGTCAAGCTGTCGCAACATCTGGCGCTTACGCCGCAATTGCAGCAGTCCATCCGGTTATTGCA
ACTCTCCACGATAGAGTTGAACCAGGAAATAGAGCGCATCGTACAGGAGAATCCCCTTCTGGAACTTGATGACAGCCTCG
GCAACGACAGTACCGATTATCATAGCCTCATACCGGAGGAGTTGGCCGCACCGTTGCCAAACGAGCTGGAGGTTGCCGAG
GAAAGCCTCCCTATTCTCTCCGTCGAAGCAGAAACCGATGTGAAGCAACCGCTTGACGAGCGGGAGTGGCCGCCGGATGA
CAGCGTTTTTCGGCCCTACCGGGACGATGAGGACGAACGTGATATTCCACAGCAAGCGGTGGAACCTCCGAATCTCCGTG
CCCACCTCAACTCGCAACTCAGCCTGAGCCAGATCTCCCAGCGGGATAGAAAGATCACCGGATTACTGATCGACAGCCTG
GATGATGATGGCTATCTTGTCCAGGACCTGGAAGAATTGGTCGATCTGTTGCCCGCCGAGCTGTCAATAGATATCGACGA
CCTGCACATTGCCCTCGAACATTTGCAGCACTTGGATCCCCCCGGCATCGGTGCGCGCAACTTGAGAGAATGCCTCGTCA
TGCAACTGCAGGCGCTCCCACCCGATACGCCCTATCTGGAGCAGGCATTGGCGCTGGTGAACAACCATCTTGAAAGTCTG
GCATCCCGGGACTTTGGCGCCATCAAGCGGGTGCTGCACTGCAACGACGACTGTCTTCGCTCTGTCCAGCAGATGATAAC
GCGACTGAATCCGAGACCGGCCACGGCCTTCAGCTCCACAGTCGCCTGCTACATCGTGCCCGACGTCATCGTGACCAAAG
TCGGCGGTTCCTGGGTAGCCAGCCTTAATCCGGAAGCCATGCCGCGTCTCAGGATCAACCGGCTCTATGCGGAGATCCTG
AAAGGATGCAATGACGACTCTACCCGTCGGCTGATCAGCCAATTGCAGGAAGCAAAATGGCTGGTAAAGAACGTACAACA
GCGTTTCAACACCATCCTCAAGGCATCGGCAGCCATTGTGGAACGCCAGCAGCAGTTTTTCGAGCATGGAGCAGTTGCGA
TGCGTCCGATGATACTGCGGGAAATCGCCGACGTGCTGAATCTGCACGAGTCCACCATTTCGCGGGTTACCACGCAGAAA
TTCATGCGCACGCCGCGCGGCATTTTCGAATTGAAGTATTTTTTTGGGAGCCACGTATCAACGGATAGTGGTGGCGCTTG
CTCCGCCACTGCCATTCGCGCGCTGATCAAGCAAATGATCAGTGAGGAAAACTCGAGGAAGCCGCTCAGCGACAGTCAGA
TCTCGGAGGTCCTGGGGCAGCAGGGTATCATGGTTGCTCGCCGTACCGTAGCAAAATATCGGGAGTCCTTGCAAATTCCT
TCCGTCAATCTTCGGAAATCGTTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(333-486)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(124-316)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.992

100

0.535

Multiple sequence alignment