Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BCEP18194_RS20875 Genome accession   NC_007510
Coordinates   3281760..3282926 (+) Length   388 a.a.
NCBI ID   WP_011353241.1    Uniprot ID   Q39CT3
Organism   Burkholderia lata     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3276760..3287926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCEP18194_RS20845 (Bcep18194_A6133) - 3277402..3278007 (+) 606 WP_011353236.1 50S ribosomal protein L25/general stress protein Ctc -
  BCEP18194_RS20850 (Bcep18194_A6134) pth 3278129..3278728 (+) 600 WP_006477776.1 aminoacyl-tRNA hydrolase -
  BCEP18194_RS20855 (Bcep18194_A6135) hisC 3278844..3279911 (+) 1068 WP_011353237.1 histidinol-phosphate transaminase -
  BCEP18194_RS20860 (Bcep18194_A6136) - 3279996..3280262 (-) 267 WP_011353238.1 YfhL family 4Fe-4S dicluster ferredoxin -
  BCEP18194_RS20865 (Bcep18194_A6137) coaD 3280322..3280819 (-) 498 WP_011353239.1 pantetheine-phosphate adenylyltransferase -
  BCEP18194_RS20870 (Bcep18194_A6138) rsmD 3280931..3281545 (-) 615 WP_011353240.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BCEP18194_RS20875 (Bcep18194_A6139) pilA 3281760..3282926 (+) 1167 WP_011353241.1 signal recognition particle-docking protein FtsY Machinery gene
  BCEP18194_RS20880 (Bcep18194_A6140) maiA 3283012..3283656 (-) 645 WP_011353242.1 maleylacetoacetate isomerase -
  BCEP18194_RS20885 (Bcep18194_A6141) ybiB 3283851..3284825 (+) 975 WP_011353243.1 DNA-binding protein YbiB -
  BCEP18194_RS20890 (Bcep18194_A6142) leuA 3285206..3286852 (+) 1647 WP_011353244.1 2-isopropylmalate synthase -
  BCEP18194_RS20895 (Bcep18194_A6143) - 3286996..3287379 (-) 384 WP_011353245.1 nuclear transport factor 2 family protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 41394.40 Da        Isoelectric Point: 4.7979

>NTDB_id=24919 BCEP18194_RS20875 WP_011353241.1 3281760..3282926(+) (pilA) [Burkholderia lata]
MFSFFKRFKKTQEPDPAESQSADAQQTDEPSDVPAVEAPPAPDVPQAPAQPVAQPAVPAVVMTVTPTNDGRDEVVETVEI
VPPPLQDASAKKSWLARLKTGLAKTGSSITGVFVNTKIDEDLYEELETALLMSDAGVDATEYLLGALREKVRTGRLTDPQ
QVKSALHDLLVELLTPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGE
RNNVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVISKAHDGAPHEVLLVIDANTGQN
ALTQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAVEFADALLG

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=24919 BCEP18194_RS20875 WP_011353241.1 3281760..3282926(+) (pilA) [Burkholderia lata]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGCCCGATCCGGCGGAATCGCAATCGGCCGACGCGCAGCAAAC
GGACGAACCGTCCGATGTGCCCGCGGTCGAGGCTCCGCCTGCGCCCGACGTGCCGCAAGCGCCCGCGCAACCGGTCGCGC
AACCGGCCGTGCCGGCCGTCGTGATGACGGTCACGCCGACCAACGACGGCCGCGACGAAGTCGTCGAGACGGTCGAAATC
GTCCCGCCGCCGCTGCAAGACGCCTCCGCGAAGAAATCATGGCTCGCGCGCCTGAAAACGGGGCTCGCCAAGACGGGCTC
GAGCATCACCGGCGTGTTCGTCAACACGAAGATCGACGAGGATCTGTACGAGGAGCTCGAAACCGCGCTGCTGATGTCCG
ACGCGGGCGTCGACGCGACCGAGTACCTGCTCGGCGCGCTGCGCGAGAAGGTGCGCACGGGCCGCCTGACCGACCCGCAG
CAGGTGAAATCCGCACTGCACGACCTGCTCGTCGAGCTGCTGACGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCA
GCCGCTCGTGATGATGATCACCGGCGTGAACGGTGCGGGCAAGACGACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGA
GCTTCGACCAATCGGTGCTGCTGGCCGCGGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAA
CGCAACAACGTGACGGTCGTGCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGC
GCGCAAGATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTCCACCTGATGGAAGAGCTGAAGAAGG
TGAAGCGCGTGATCTCGAAGGCGCATGACGGCGCGCCGCACGAAGTGCTGCTGGTGATCGACGCGAACACCGGCCAGAAC
GCGCTCACGCAGGTCAAGGCATTCGACGACGCGCTCGGCCTCACCGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAA
GGGCGGGATTCTCGCCGCGATCGCGCGGCAGCGCCCGGTGCCGGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACC
TGCAGCCGTTCAGCGCGGTCGAATTCGCGGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(189-388)

SRP54-type protein, helical bundle domain (SRP54_N)

(106-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q39CT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

54.306

100

0.585

Multiple sequence alignment