Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   CPF12_RS02660 Genome accession   NZ_CP023643
Coordinates   561972..563780 (-) Length   602 a.a.
NCBI ID   WP_069125706.1    Uniprot ID   A0A1D2KUE0
Organism   Brochothrix thermosphacta strain BII     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 556972..568780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPF12_RS02650 (CPF12_02650) - 558847..559575 (-) 729 WP_029091616.1 amino acid ABC transporter ATP-binding protein -
  CPF12_RS12895 (CPF12_02655) - 559556..561718 (-) 2163 WP_069125705.1 amino acid ABC transporter substrate-binding protein/permease -
  CPF12_RS02660 (CPF12_02660) pepF 561972..563780 (-) 1809 WP_069125706.1 oligoendopeptidase F Regulator
  CPF12_RS02665 (CPF12_02665) - 563857..564969 (-) 1113 WP_069125708.1 competence protein CoiA -
  CPF12_RS02670 (CPF12_02670) - 565167..566468 (+) 1302 WP_096699235.1 ISL3 family transposase -
  CPF12_RS02675 (CPF12_02675) mecA 566721..567401 (-) 681 WP_069119615.1 adaptor protein MecA -
  CPF12_RS02680 (CPF12_02680) spxA 567660..568067 (-) 408 WP_029091611.1 transcriptional regulator SpxA -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 67863.91 Da        Isoelectric Point: 4.6006

>NTDB_id=248846 CPF12_RS02660 WP_069125706.1 561972..563780(-) (pepF) [Brochothrix thermosphacta strain BII]
MAGKAITRSEVPVKDTWDLTSIFETDEAWETEFKAVQVLAEEATKFQKTLGDSADQLLAALKYSDELVVRLSQVYVYASM
KHDQDTSNTEYQALNSRAESLAAKASAALSFFEPELLDIDEAVLAAFISESDELGVYAHELEQINKKRPHTLSAPEEALL
AKASEVLGSSQNTFGILNNADLRFPTIKDENGEDVEITHGLYGPLLENSDRRVRRDAFQNVYKTYKSLLNTFASTLTGQI
KQDNFNASVRKFNTARESAVFSNNIPESVHKTLLETVGNNVDLLHRYVSLRKQVLGLDELHVYDLYTPLVADVDELSFTY
EEAKQVTINALAPLGEEYVSILKEGFDKRWVDVVENKGKRSGAYSGGSYGTNPFILLNWHDTLDNLYTLVHEFGHSVHSY
LTRKNQPAVYGDYTIFLAEIASTTNENLLTDYLLKKYDDKKIQAYIINHFLDGFKGTVFRQTQFAEFEHLIHVADQEGNA
LTAEYLTDLYYGINKKYYGDALVHDEEIGNEWARIPHFYYNYYVYQYATGFSAASALAQHILDEGAPAVERYMTFLKAGN
SDYPIDVLKAAGVDMTTAKPIEDALKVFEARLAEFEAIIIEK

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=248846 CPF12_RS02660 WP_069125706.1 561972..563780(-) (pepF) [Brochothrix thermosphacta strain BII]
ATGGCTGGAAAAGCAATTACACGCTCTGAAGTGCCTGTAAAAGACACTTGGGACTTAACAAGTATTTTTGAAACAGATGA
AGCATGGGAAACAGAATTTAAAGCTGTCCAAGTATTAGCAGAAGAAGCAACTAAATTCCAAAAAACATTAGGTGATAGTG
CAGACCAGCTTCTTGCAGCTTTAAAATATAGTGATGAGCTTGTTGTTCGTTTATCACAAGTTTATGTTTATGCAAGTATG
AAACATGATCAAGATACTAGCAATACAGAGTATCAAGCGTTAAATAGTCGTGCAGAATCATTGGCGGCCAAAGCCTCAGC
TGCACTTTCTTTCTTTGAACCGGAATTATTAGATATTGATGAAGCGGTACTTGCCGCCTTTATTTCTGAGTCTGACGAAT
TAGGTGTATATGCGCATGAGTTAGAACAAATCAATAAGAAACGTCCTCATACATTGTCTGCTCCTGAGGAAGCGTTATTA
GCTAAAGCAAGCGAAGTATTAGGTTCATCTCAAAACACTTTTGGTATTCTAAATAATGCAGATTTACGTTTTCCAACAAT
CAAAGATGAAAATGGTGAAGATGTAGAGATTACACACGGTCTATACGGTCCATTATTGGAAAATAGCGATCGTCGTGTGC
GTCGTGACGCTTTCCAAAATGTGTATAAAACGTATAAGTCATTGCTTAATACATTTGCCTCAACGTTGACTGGTCAAATT
AAGCAAGATAACTTCAATGCATCTGTTCGTAAGTTTAATACTGCCAGAGAGTCGGCTGTTTTCAGCAATAATATTCCTGA
GTCAGTTCATAAAACATTGCTTGAAACGGTAGGGAATAATGTTGATTTATTACATCGTTATGTGAGTTTACGCAAGCAGG
TATTAGGTCTTGACGAATTGCATGTTTACGATCTTTACACACCGCTTGTAGCGGATGTAGATGAGCTTTCATTTACGTAT
GAAGAAGCTAAACAAGTAACAATTAATGCCTTAGCACCTTTGGGTGAAGAATATGTTTCTATCCTTAAAGAAGGTTTTGA
TAAGCGTTGGGTAGATGTGGTTGAAAATAAAGGGAAACGTAGTGGCGCATACTCTGGCGGTTCATACGGTACCAATCCAT
TTATTCTATTAAACTGGCATGATACATTAGATAATTTGTATACATTAGTTCATGAGTTTGGGCACAGTGTGCATAGTTAT
CTCACTCGAAAAAATCAACCGGCAGTTTATGGCGATTATACAATCTTTTTAGCAGAGATTGCTTCAACTACCAATGAAAA
TTTATTGACAGATTATCTTCTGAAAAAATATGATGATAAAAAAATCCAAGCCTATATTATTAACCATTTCCTTGATGGTT
TTAAAGGTACTGTTTTCCGTCAAACACAATTTGCAGAATTTGAGCACCTTATTCATGTTGCTGATCAAGAAGGTAACGCT
TTAACAGCTGAATATCTAACTGATCTCTACTATGGTATTAATAAGAAATATTATGGTGATGCACTGGTACACGATGAAGA
AATTGGTAACGAATGGGCGCGTATTCCTCATTTCTACTACAATTATTACGTGTATCAATATGCAACTGGTTTTTCTGCAG
CTTCAGCTTTGGCACAACATATTTTGGACGAAGGAGCTCCAGCAGTAGAACGTTACATGACGTTCTTAAAAGCAGGTAAC
TCGGATTATCCAATCGATGTGTTGAAAGCTGCTGGCGTTGACATGACTACTGCAAAACCAATTGAGGATGCTTTGAAAGT
TTTCGAAGCACGTCTAGCTGAATTCGAAGCAATTATTATAGAAAAATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(115-184)

Peptidase family M3 (Peptidase_M3)

(205-586)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D2KUE0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.689

98.339

0.508

Multiple sequence alignment