Detailed information NTG0247539

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: COP00_RS22470
• Genome accession: NZ_CP023481
• Coordinates: 4282416..4286108 (+)
• Length: 1230 a.a.
• NCBI ID: WP_048356283.1
• Uniprot ID: A0A0J6HFJ6
• Organism: Bacillus glycinifermentans strain KBN06P03352
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4277416..4291108

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
COP00_RS22460 (COP00_22460)	modB	4278164..4278820 (+)	657	WP_048355538.1	molybdate ABC transporter permease subunit	-
COP00_RS22465 (COP00_22465)	addB	4278929..4282429 (+)	3501	WP_048406707.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
COP00_RS22470 (COP00_22470)	addA	4282416..4286108 (+)	3693	WP_048356283.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
COP00_RS22475 (COP00_22475)	sbcD	4286176..4287360 (+)	1185	WP_048356282.1	exonuclease subunit SbcD	-
COP00_RS22480 (COP00_22480)	sbcC	4287357..4290749 (+)	3393	WP_057957617.1	exonuclease subunit SbcC	-
COP00_RS22485 (COP00_22485)	-	4290809..4291027 (-)	219	WP_046131288.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141273.15 Da
• Isoelectric Point: 5.7739

>NTDB_id=247539 COP00_RS22470 WP_048356283.1 4282416..4286108(+) (addA) [Bacillus glycinifermentans strain KBN06P03352]
METAKPKNSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIGEA
LEKKLAENPGSLHLRRQLSLLNRASISTLHSFCLQVVRKYYYMIDVDPAFRIADQTEGELLGDEVLDELFEEEYQKGDPA
FFELVDRYTTDRHDLDLQYLIKRVYEFSRSHPDPEAWLEKFMDLYDADPGAKIESLPFYSYIREDIALVLNSVREKLTRA
LELTKRPGGPAPRAENFLDDLAQIDKLLEHQDDFAALYELVPAVSFQRAKPCKGDDFDPSLIDEATDLRNSAKKQLEKLK
GDYFTRTPEQHLASLKEMKPVIRTLVQLVAEFGRRYRAAKKEKAIVDFSDLEHDCLAILTKKNEGGTAEPSEAAAYYRHL
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGAGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVAYDEQAELKLGASYPPNESTKTEVLIIENREDAADEEAEELETVQLEARAVAGRIREL
ISGKFQVYDGKTKTTRNIQYRDIVILLRSMPWAPQFMDEFKQQGIPVYANLSTGYFEATEVAVTLSLLKVIDNAYQDIPL
ASILRSPIVGLDENELSLIRIRDKKAPFYEALKAYMASADKDDPLYEKLARFDGFLKKWRAYAKNHSVAELIWEVYRDTK
YIDYVGGMPGGKQRQANLRALYDRARSYESTSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFLVG
TAKDKDKLLAKWRTSASHAEWLLPDFERFQAKSYLDFIGPALMRHRDMADGDLNLPNDDIRQHQSRFTISWISAGELQAE
GRAQTEEEKHERLSLVKKGLPIDGSFDYEKEVRERLTWSYPFRDASQVRTKQSVSEIKRQKEYEDEYGDRSLVRPANETL
LFRRPSFMMAKGLTAAERGTAMHTVMQHLPLTRVPEKDELGRMLDRLIEKELLTEEQRAAIEEDDILAFFDTDIGGKMLS
ARRVEREVPFNMTLPAREVYPGLKTADEPVLIQGIIDCLFETDDGFYLLDYKTDRIHGKFQNGFEGAEPILRKRYATQIN
LYARAVETMLKTPLRGKALYFFDGGHVIMF

Nucleotide

• Length: 3693 bp

>NTDB_id=247539 COP00_RS22470 WP_048356283.1 4282416..4286108(+) (addA) [Bacillus glycinifermentans strain KBN06P03352]
ATGGAAACGGCTAAGCCGAAAAACAGCACATGGACGGACGACCAATGGAAAGCGATCGTCTCATCCGGCCGGGATATTTT
GGTCGCCGCTGCGGCGGGCTCCGGAAAAACGGCCGTCCTTGTCGAGCGGATCATCCGAAAGATTACGGCGGAGGAAAATC
CGATCGATGTCGACCGGCTGCTCGTCGTCACCTTTACGAATGCATCGGCGGCGGAGATGAAGCACCGGATCGGTGAAGCG
CTTGAGAAAAAGCTCGCCGAAAACCCCGGCTCCCTTCATTTGCGCAGGCAGCTTTCCTTGCTGAACAGGGCGAGTATTTC
GACATTGCACTCTTTTTGCCTTCAGGTCGTCCGCAAGTATTATTATATGATCGACGTTGATCCGGCCTTTCGGATCGCCG
ATCAGACAGAAGGCGAGCTGCTCGGTGATGAGGTGCTTGACGAGCTGTTTGAAGAAGAATATCAAAAGGGCGATCCGGCT
TTTTTTGAGCTGGTGGACAGATATACGACAGACCGCCACGATCTCGATTTGCAGTACCTTATCAAGCGGGTTTACGAGTT
TTCCCGCTCACACCCCGACCCGGAAGCGTGGCTTGAAAAATTTATGGACCTGTATGACGCAGATCCCGGCGCGAAGATCG
AGTCGCTTCCGTTTTATTCCTACATCAGGGAAGATATCGCGCTTGTATTGAACAGTGTGCGTGAAAAGCTGACCCGTGCG
CTTGAGCTGACAAAACGGCCGGGGGGGCCGGCTCCGCGCGCTGAAAACTTTCTCGACGATTTGGCGCAGATCGACAAGCT
GCTTGAGCATCAGGACGATTTTGCGGCCCTTTATGAGCTTGTGCCGGCTGTTTCGTTCCAGCGGGCCAAACCGTGCAAAG
GGGATGATTTCGATCCGTCTTTAATCGACGAAGCGACAGACCTTCGCAACAGTGCAAAAAAACAGCTTGAAAAGCTGAAA
GGCGACTATTTCACGCGAACGCCAGAGCAGCATTTGGCGAGTTTGAAGGAGATGAAGCCTGTCATCAGGACGCTTGTCCA
GCTCGTTGCAGAGTTCGGCAGACGCTATAGGGCCGCGAAGAAAGAAAAAGCGATCGTAGACTTCTCCGATCTTGAGCACG
ACTGCCTGGCGATTTTGACAAAGAAAAACGAAGGCGGAACAGCGGAGCCGAGCGAAGCTGCGGCCTATTACCGGCACTTG
TTCCACGAAGTGCTCGTTGATGAATATCAGGATACAAACCTTGTACAGGAGGCGATTTTAAAGCTTGTCGCCAAGGAGGA
ACACGAAGGGAACCTCTTTATGGTGGGCGATGTCAAGCAGTCGATCTACCGTTTCCGGCTGGCTGAGCCGCTTTTGTTTC
TTTCGAAATACAAGCGGTTCACAGATGACGGCTCAGGCGCCGGACAGAAAATCGATTTGAACAAAAACTTCAGAAGCCGG
TCCGACATTTTGGACAGCACCAATTTTTTGTTTAAACAGCTGATGGGAGAGAAGGTCGGCGAGGTTGCCTATGACGAACA
AGCCGAATTAAAGCTCGGCGCATCGTATCCTCCCAATGAAAGCACAAAAACAGAGGTGCTGATCATTGAAAACCGGGAAG
ACGCGGCTGATGAAGAGGCGGAAGAGCTTGAAACGGTACAGCTTGAGGCAAGGGCTGTCGCCGGACGGATCAGGGAGCTG
ATTTCCGGCAAATTTCAAGTGTATGACGGTAAAACGAAAACGACAAGAAACATCCAGTACCGCGACATCGTGATTTTGCT
GCGCTCCATGCCGTGGGCGCCGCAATTCATGGACGAGTTCAAGCAGCAGGGGATTCCGGTATACGCGAACCTGTCAACCG
GATATTTCGAAGCGACAGAAGTAGCCGTCACGCTTTCACTGCTTAAAGTCATCGACAATGCCTATCAGGATATTCCGCTT
GCATCCATCCTCAGGTCACCGATTGTCGGCCTTGATGAAAACGAACTGTCCCTGATCAGAATCCGGGATAAAAAAGCGCC
GTTCTATGAAGCGCTGAAGGCGTATATGGCGTCAGCGGACAAAGATGACCCGCTTTATGAAAAGCTTGCCCGCTTTGACG
GGTTCCTGAAAAAGTGGAGGGCATACGCAAAAAATCATTCCGTGGCGGAGCTGATATGGGAAGTTTACCGCGATACGAAA
TATATCGATTATGTCGGCGGAATGCCGGGCGGAAAGCAGCGGCAGGCCAATTTGCGCGCGCTCTATGACAGGGCGAGATC
GTACGAATCGACCTCCTTCCGCGGATTGTTCCGCTTTTTGCGATTTATTGAACGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCTCGGGCTTTGAGCGAACAGGAAGACGTCGTCCGCCTGATGACGATCCATTCGAGCAAAGGTCTTGAATTTCCC
GTCGTATTCACGGCAGGCCTCGGCCGGAATTTCAATATGATGGATCTCAACAAATCGTATCTGCTTGATAAAGAGCTCGG
GTTCGGGACGAAATTTATCCATCCGAAATGGCGCATCAGCTATCCGACGCTGCCGCTTGTTGCGATGAAGAAAAAAATGC
GGCGCGAGCTTTTATCAGAAGAGCTTCGCGTCTTGTATGTCGCTCTCACCCGTGCAAAGGAAAAACTCTTTTTGGTCGGA
ACGGCGAAAGACAAGGACAAGCTTTTGGCAAAATGGAGGACCTCCGCCTCCCATGCCGAATGGCTGCTCCCTGATTTTGA
ACGCTTTCAGGCGAAATCGTATCTCGATTTTATCGGCCCCGCCTTAATGCGGCACAGGGATATGGCAGACGGCGATCTGA
ACTTGCCGAATGATGACATCCGTCAGCATCAGTCCCGTTTTACGATCAGCTGGATATCAGCCGGAGAGCTGCAGGCCGAA
GGTAGAGCGCAGACTGAAGAAGAAAAACACGAACGGCTGTCACTGGTGAAAAAAGGGCTGCCGATCGATGGCTCTTTCGA
TTATGAAAAAGAGGTCCGCGAGCGGCTGACATGGTCGTATCCTTTCCGGGATGCGTCACAAGTCCGCACAAAACAGTCTG
TCTCTGAAATCAAGAGGCAGAAGGAATACGAGGATGAATACGGCGACCGCTCATTGGTTCGTCCGGCAAATGAAACTTTG
CTGTTCAGAAGACCCTCTTTCATGATGGCAAAAGGGCTGACCGCCGCCGAACGGGGAACGGCGATGCATACAGTCATGCA
GCATCTTCCGCTCACCCGCGTCCCCGAAAAGGATGAGCTCGGCCGTATGCTGGACAGGCTTATCGAAAAAGAACTTCTAA
CGGAAGAGCAACGCGCGGCTATCGAAGAAGACGACATTCTCGCGTTTTTCGATACGGATATAGGCGGAAAAATGCTCAGC
GCCCGCCGGGTTGAGCGGGAAGTGCCCTTCAACATGACGCTTCCTGCACGAGAGGTTTATCCGGGCTTGAAAACTGCCGA
TGAACCCGTCTTAATTCAAGGGATTATCGACTGTTTGTTCGAAACCGATGACGGCTTTTATTTGCTCGACTACAAAACCG
ACCGGATACACGGCAAATTTCAAAACGGCTTTGAAGGAGCGGAGCCGATTTTGCGAAAACGCTACGCCACACAGATCAAC
CTTTACGCCCGGGCTGTTGAAACGATGCTTAAGACGCCTCTGAGAGGTAAGGCTCTTTACTTTTTCGACGGCGGGCACGT
GATCATGTTTTGA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

3D structure

• AlphaFold DB: A0A0J6HFJ6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	73.577	100	0.736