Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CLI98_RS11345 Genome accession   NZ_CP023431
Coordinates   2221070..2221507 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SCGB 574     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2216070..2226507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI98_RS11320 (CLI98_02231) - 2217090..2218226 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  CLI98_RS11325 (CLI98_02232) - 2218241..2218675 (+) 435 WP_014416871.1 RDD family protein -
  CLI98_RS11330 (CLI98_02233) - 2218747..2219070 (+) 324 WP_007409357.1 YckD family protein -
  CLI98_RS11335 (CLI98_02234) - 2219174..2220610 (+) 1437 WP_022552568.1 family 1 glycosylhydrolase -
  CLI98_RS11340 (CLI98_02235) nin/comJ 2220651..2221049 (-) 399 WP_022552569.1 competence protein ComJ Regulator
  CLI98_RS11345 (CLI98_02236) nucA/comI 2221070..2221507 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CLI98_RS11350 (CLI98_02237) hxlB 2221838..2222395 (-) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  CLI98_RS11355 (CLI98_02238) hxlA 2222392..2223027 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  CLI98_RS11360 (CLI98_02239) - 2223259..2223621 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=247081 CLI98_RS11345 WP_003156588.1 2221070..2221507(-) (nucA/comI) [Bacillus velezensis strain SCGB 574]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=247081 CLI98_RS11345 WP_003156588.1 2221070..2221507(-) (nucA/comI) [Bacillus velezensis strain SCGB 574]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCCGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment