Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   CLI97_RS16365 Genome accession   NZ_CP023320
Coordinates   3109214..3111043 (+) Length   609 a.a.
NCBI ID   WP_003155028.1    Uniprot ID   -
Organism   Bacillus velezensis strain SCGB     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 3104214..3116043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI97_RS16340 (CLI97_03223) - 3104877..3105455 (+) 579 WP_003155035.1 GNAT family N-acetyltransferase -
  CLI97_RS16345 (CLI97_03224) spx 3105634..3106029 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  CLI97_RS16350 (CLI97_03225) - 3106087..3106743 (-) 657 WP_003155033.1 TerC family protein -
  CLI97_RS21040 - 3106926..3107036 (+) 111 Protein_3154 adaptor protein MecA -
  CLI97_RS16355 (CLI97_03226) mecA 3107019..3107675 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  CLI97_RS16360 (CLI97_03227) - 3107826..3108986 (+) 1161 WP_094247076.1 competence protein CoiA family protein -
  CLI97_RS16365 (CLI97_03228) pepF 3109214..3111043 (+) 1830 WP_003155028.1 oligoendopeptidase F Regulator
  CLI97_RS21045 (CLI97_03229) - 3111081..3111248 (-) 168 WP_003155026.1 hypothetical protein -
  CLI97_RS16375 (CLI97_03230) spxH 3111534..3112436 (-) 903 WP_024085126.1 protease adaptor protein SpxH -
  CLI97_RS16380 (CLI97_03231) - 3112433..3112831 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  CLI97_RS16385 (CLI97_03232) - 3113060..3113746 (-) 687 WP_017417458.1 lytic transglycosylase domain-containing protein -
  CLI97_RS16390 (CLI97_03233) - 3113751..3114326 (-) 576 WP_003155021.1 CYTH domain-containing protein -
  CLI97_RS16395 (CLI97_03234) - 3114451..3114816 (+) 366 WP_003155020.1 hypothetical protein -
  CLI97_RS16400 (CLI97_03235) yjbM 3114844..3115479 (+) 636 WP_003155019.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69799.43 Da        Isoelectric Point: 5.2024

>NTDB_id=246069 CLI97_RS16365 WP_003155028.1 3109214..3111043(+) (pepF) [Bacillus velezensis strain SCGB]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=246069 CLI97_RS16365 WP_003155028.1 3109214..3111043(+) (pepF) [Bacillus velezensis strain SCGB]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATTCCGAATTTATCTTCTTTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCACATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCTCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TGGAAAAAGAGGAGCTTAAGCTTTACTCCCATGCAATTGAGGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCACCTTCCAATACATTCAGCGTTTTAAATAACGCAGATAT
TACGTTTCCGTCGATTAAGGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAATATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATTCCTGAAGAGGTTTACGATAACCTTATCAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGACAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCTAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGGTTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTAACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGTTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAATGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488

Multiple sequence alignment