Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VF_RS11925 Genome accession   NC_006840
Coordinates   2579066..2580067 (-) Length   333 a.a.
NCBI ID   WP_011262704.1    Uniprot ID   Q5E2F4
Organism   Aliivibrio fischeri ES114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2574066..2585067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS11900 (VF2292) aroK 2574908..2575426 (-) 519 WP_005421091.1 shikimate kinase AroK -
  VF_RS11905 (VF2293) pilQ 2575651..2577339 (-) 1689 WP_047863557.1 type IV pilus secretin PilQ Machinery gene
  VF_RS11910 (VF2294) pilP 2577426..2577944 (-) 519 WP_011262701.1 pilus assembly protein PilP Machinery gene
  VF_RS11915 (VF2295) pilO 2577937..2578515 (-) 579 WP_011262702.1 type 4a pilus biogenesis protein PilO Machinery gene
  VF_RS11920 (VF2296) pilN 2578515..2579081 (-) 567 WP_011262703.1 PilN domain-containing protein Machinery gene
  VF_RS11925 (VF2297) pilM 2579066..2580067 (-) 1002 WP_011262704.1 type IV pilus biogenesis protein PilM Machinery gene
  VF_RS11930 (VF2298) - 2580202..2582700 (+) 2499 WP_011262705.1 penicillin-binding protein 1A -
  VF_RS11935 (VF2299) oxyR 2582767..2583666 (-) 900 WP_011262706.1 DNA-binding transcriptional regulator OxyR -
  VF_RS11940 (VF2300) - 2583847..2584575 (+) 729 WP_011262707.1 redoxin family protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37266.35 Da        Isoelectric Point: 6.9332

>NTDB_id=24589 VF_RS11925 WP_011262704.1 2579066..2580067(-) (pilM) [Aliivibrio fischeri ES114]
MIYPTITGLDVGHHSIKAVSVRLKKGQLDLVSSFEVLLPESVFVDTNNLNVEELKPYLSRIKKQLKLNQKQVAFSIPDSS
ITSKVVQIDSQLDERETEFAIAHNFEQHSSFSSDDLNIDYVASEHRGTGSLQTITYQVFATRKDLVNSRQRCFESAGLKP
VLADAHSNALLTLWQRAIAVYPDKKNWMLIDIGYMQTVFCIASPSQHLYSKHILFGAIAQEKETNSTNEDNSIDLSAGTL
FIKQLSEHIHRQVTLYSSVNQHKVEGVWITGGGSMLPGLSDSLSYELSLPTVDLNLLSLFQTPKKRVGSFHEQKNQYASA
LGLALRGIEWLTK

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=24589 VF_RS11925 WP_011262704.1 2579066..2580067(-) (pilM) [Aliivibrio fischeri ES114]
ATGATCTACCCCACTATTACAGGTTTAGATGTAGGGCATCATAGCATTAAAGCCGTATCTGTAAGGTTAAAGAAAGGTCA
ATTAGATCTCGTTAGTTCTTTCGAAGTTTTATTGCCTGAATCTGTATTTGTGGATACTAATAATTTAAATGTCGAAGAAT
TAAAGCCTTACTTATCTCGAATAAAGAAACAGTTAAAGCTAAATCAAAAGCAAGTCGCGTTCTCTATCCCTGATAGCAGT
ATTACAAGCAAGGTTGTTCAAATTGATAGTCAACTTGATGAGCGAGAAACCGAGTTTGCTATTGCTCATAATTTTGAACA
GCACTCTTCTTTTTCATCTGATGATCTGAATATAGATTATGTAGCCTCTGAACATAGAGGAACAGGCAGCTTACAGACGA
TCACTTACCAAGTTTTTGCGACCCGAAAAGATTTAGTTAACTCTCGTCAGCGTTGTTTTGAATCTGCTGGACTTAAACCT
GTACTGGCAGATGCTCATTCAAATGCACTTTTAACCTTATGGCAGAGAGCGATAGCGGTTTATCCCGATAAAAAAAATTG
GATGCTCATTGATATTGGTTATATGCAAACTGTTTTTTGTATTGCTTCTCCGAGTCAACATTTATATAGCAAACACATTT
TATTTGGTGCTATAGCTCAAGAGAAAGAGACAAATTCAACTAACGAAGATAACTCAATTGATCTGTCCGCTGGGACGCTT
TTTATTAAGCAGTTATCTGAACATATTCATCGTCAAGTGACTCTTTATTCATCGGTTAATCAACATAAGGTTGAAGGCGT
ATGGATTACTGGTGGTGGCTCAATGCTACCAGGGTTATCGGATTCGTTAAGCTACGAACTATCGTTACCAACGGTGGATT
TAAATCTACTTTCTTTGTTTCAAACACCAAAGAAAAGAGTGGGTTCGTTTCATGAACAAAAAAATCAATACGCATCAGCT
CTTGGATTGGCATTGAGAGGGATTGAATGGCTTACCAAATAA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-327)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5E2F4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

38.415

98.498

0.378

Multiple sequence alignment