Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VF_RS11920 Genome accession   NC_006840
Coordinates   2578515..2579081 (-) Length   188 a.a.
NCBI ID   WP_011262703.1    Uniprot ID   Q5E2F5
Organism   Aliivibrio fischeri ES114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2573515..2584081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS11895 (VF2291) aroB 2573783..2574871 (-) 1089 WP_011262699.1 3-dehydroquinate synthase -
  VF_RS11900 (VF2292) aroK 2574908..2575426 (-) 519 WP_005421091.1 shikimate kinase AroK -
  VF_RS11905 (VF2293) pilQ 2575651..2577339 (-) 1689 WP_047863557.1 type IV pilus secretin PilQ Machinery gene
  VF_RS11910 (VF2294) pilP 2577426..2577944 (-) 519 WP_011262701.1 pilus assembly protein PilP Machinery gene
  VF_RS11915 (VF2295) pilO 2577937..2578515 (-) 579 WP_011262702.1 type 4a pilus biogenesis protein PilO Machinery gene
  VF_RS11920 (VF2296) pilN 2578515..2579081 (-) 567 WP_011262703.1 PilN domain-containing protein Machinery gene
  VF_RS11925 (VF2297) pilM 2579066..2580067 (-) 1002 WP_011262704.1 type IV pilus biogenesis protein PilM Machinery gene
  VF_RS11930 (VF2298) - 2580202..2582700 (+) 2499 WP_011262705.1 penicillin-binding protein 1A -
  VF_RS11935 (VF2299) oxyR 2582767..2583666 (-) 900 WP_011262706.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 188 a.a.        Molecular weight: 21742.03 Da        Isoelectric Point: 9.3705

>NTDB_id=24588 VF_RS11920 WP_011262703.1 2578515..2579081(-) (pilN) [Aliivibrio fischeri ES114]
MAYQINLLPWREKQRARYKQRFIALLCAGVTLSVGIQWGIAQYLEQQVSIQTQRKQSLEKHISWLNNELKDLNEVGKEHE
AILTRLDVVEQLQQNRNKTTQLLNILPDIITEGIYLNKVRMHEREVEIKGFSDSNSRLASMLDKLERSSQITSVEMHSII
SGQKIFGHDVSSFEVSFKLLPPVKKEPS

Nucleotide


Download         Length: 567 bp        

>NTDB_id=24588 VF_RS11920 WP_011262703.1 2578515..2579081(-) (pilN) [Aliivibrio fischeri ES114]
ATGGCTTACCAAATAAACCTATTACCTTGGCGAGAAAAGCAGAGAGCAAGATATAAGCAACGTTTTATTGCATTACTTTG
TGCTGGGGTGACGCTATCTGTCGGTATTCAATGGGGGATTGCGCAATATTTAGAACAACAAGTTTCGATTCAGACACAAC
GTAAGCAATCGTTAGAAAAACATATCTCTTGGTTAAACAATGAATTAAAAGATCTTAACGAAGTTGGAAAGGAGCATGAA
GCGATATTAACTCGTCTTGATGTTGTCGAACAATTACAGCAGAACCGAAACAAAACCACTCAGTTATTGAACATTTTACC
CGACATCATTACTGAAGGTATTTATTTAAATAAAGTACGCATGCATGAAAGGGAAGTTGAGATTAAGGGATTCAGTGATA
GTAACTCTCGGCTAGCAAGTATGTTGGATAAATTAGAGCGTTCCTCTCAAATTACTTCAGTAGAAATGCACTCGATTATC
TCAGGGCAAAAAATATTTGGTCATGATGTCAGTAGTTTTGAAGTCTCGTTTAAATTACTTCCTCCAGTGAAAAAGGAACC
GTCGTAA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5E2F5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

47.541

97.34

0.463
  pilN Vibrio campbellii strain DS40M4

46.328

94.149

0.436

Multiple sequence alignment