Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   CLD04_RS09385 Genome accession   NZ_CP023257
Coordinates   1776653..1777546 (+) Length   297 a.a.
NCBI ID   WP_017694865.1    Uniprot ID   -
Organism   Bacillus subtilis strain TLO3     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1771653..1782546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLD04_RS09365 (CLD04_09365) - 1772323..1774053 (+) 1731 WP_017694863.1 hypothetical protein -
  CLD04_RS09370 (CLD04_09370) ylqH 1774050..1774331 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  CLD04_RS09375 (CLD04_09375) sucC 1774504..1775661 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  CLD04_RS09380 (CLD04_09380) sucD 1775690..1776592 (+) 903 WP_017694864.1 succinate--CoA ligase subunit alpha -
  CLD04_RS09385 (CLD04_09385) dprA 1776653..1777546 (+) 894 WP_017694865.1 DNA-processing protein DprA Machinery gene
  CLD04_RS09390 (CLD04_09390) topA 1777734..1779809 (+) 2076 WP_014479776.1 type I DNA topoisomerase -
  CLD04_RS09395 (CLD04_09395) trmFO 1779885..1781192 (+) 1308 WP_017694866.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  CLD04_RS09400 (CLD04_09400) xerC 1781260..1782174 (+) 915 WP_003231988.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32990.30 Da        Isoelectric Point: 9.8131

>NTDB_id=245540 CLD04_RS09385 WP_017694865.1 1776653..1777546(+) (dprA) [Bacillus subtilis strain TLO3]
MDQAAVCLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQLYPKLQRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKDWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=245540 CLD04_RS09385 WP_017694865.1 1776653..1777546(+) (dprA) [Bacillus subtilis strain TLO3]
TTGGATCAGGCCGCTGTCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCAGATCCGTCTATGTCGCTGACATCACCCGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAACTTTATCCAAAGCTCCAGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCCGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTGGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGATTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGACCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACGAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTATTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

98.99

100

0.99
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377

Multiple sequence alignment