Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CJ306_RS22200 Genome accession   NZ_CP023245
Coordinates   4268162..4269205 (-) Length   347 a.a.
NCBI ID   WP_001013170.1    Uniprot ID   A0A9Q5QS48
Organism   Bacillus cereus strain HBL-AI     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4263162..4274205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ306_RS22155 (CJ306_22725) - 4263595..4263795 (-) 201 WP_000106083.1 YqzE family protein -
  CJ306_RS22160 (CJ306_22730) - 4263834..4264331 (-) 498 WP_119683923.1 shikimate kinase -
  CJ306_RS22165 (CJ306_22735) - 4264447..4265097 (-) 651 WP_000183856.1 2OG-Fe(II) oxygenase -
  CJ306_RS22170 (CJ306_22740) comGG 4265272..4265643 (-) 372 WP_119683924.1 competence type IV pilus minor pilin ComGG -
  CJ306_RS22175 (CJ306_22745) comGF 4265640..4266110 (-) 471 WP_000923097.1 competence type IV pilus minor pilin ComGF -
  CJ306_RS22180 (CJ306_22750) - 4266080..4266382 (-) 303 WP_000829469.1 hypothetical protein -
  CJ306_RS22185 (CJ306_22755) comGD 4266375..4266830 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  CJ306_RS22190 (CJ306_22760) comGC 4266827..4267126 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  CJ306_RS22195 (CJ306_22765) comGB 4267138..4268175 (-) 1038 WP_282023896.1 competence type IV pilus assembly protein ComGB -
  CJ306_RS22200 (CJ306_22770) comGA 4268162..4269205 (-) 1044 WP_001013170.1 competence type IV pilus ATPase ComGA Machinery gene
  CJ306_RS22205 (CJ306_22775) - 4269411..4270106 (+) 696 WP_098620363.1 helix-turn-helix domain-containing protein -
  CJ306_RS22210 (CJ306_22780) - 4270232..4270474 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  CJ306_RS22215 (CJ306_22785) - 4270582..4271976 (+) 1395 WP_001094345.1 L-cystine transporter -
  CJ306_RS22220 (CJ306_22790) - 4272056..4272448 (-) 393 WP_063537442.1 hypothetical protein -
  CJ306_RS22225 (CJ306_22795) - 4272536..4272751 (-) 216 WP_001008326.1 DUF3912 family protein -
  CJ306_RS22230 (CJ306_22800) - 4273004..4273315 (+) 312 WP_065222860.1 hypothetical protein -
  CJ306_RS22235 (CJ306_22805) - 4273350..4273838 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39095.50 Da        Isoelectric Point: 8.7212

>NTDB_id=245378 CJ306_RS22200 WP_001013170.1 4268162..4269205(-) (comGA) [Bacillus cereus strain HBL-AI]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=245378 CJ306_RS22200 WP_001013170.1 4268162..4269205(-) (comGA) [Bacillus cereus strain HBL-AI]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

40.062

92.795

0.372

Multiple sequence alignment