Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CJ010_RS03425 Genome accession   NZ_CP022958
Coordinates   714446..715903 (-) Length   485 a.a.
NCBI ID   WP_141016744.1    Uniprot ID   A0A4Y6KMW5
Organism   Azoarcus sp. DD4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 709446..720903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ010_RS03400 (CJ010_03365) - 709955..710758 (-) 804 WP_141016739.1 ABC transporter substrate-binding protein -
  CJ010_RS03405 (CJ010_03370) ilvA 710929..712449 (-) 1521 WP_141016740.1 threonine ammonia-lyase, biosynthetic -
  CJ010_RS03410 (CJ010_03375) hprK 712577..713518 (-) 942 WP_141016741.1 HPr(Ser) kinase/phosphatase -
  CJ010_RS03415 (CJ010_03380) ptsN 713487..713972 (-) 486 WP_141016742.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CJ010_RS03420 (CJ010_03385) hpf 714109..714432 (-) 324 WP_141016743.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CJ010_RS03425 (CJ010_03390) rpoN/rpoN1 714446..715903 (-) 1458 WP_141016744.1 RNA polymerase factor sigma-54 Machinery gene
  CJ010_RS03430 (CJ010_03395) lptB 715916..716638 (-) 723 WP_141016745.1 LPS export ABC transporter ATP-binding protein -
  CJ010_RS03435 (CJ010_03400) recX 716625..717074 (-) 450 WP_141016746.1 recombination regulator RecX -
  CJ010_RS03440 (CJ010_03405) recA 717092..718126 (-) 1035 WP_141016747.1 recombinase RecA Machinery gene
  CJ010_RS03445 (CJ010_03410) - 718325..718810 (-) 486 WP_371415682.1 CinA family protein -
  CJ010_RS03450 (CJ010_03415) - 718813..719295 (-) 483 WP_141016749.1 phosphatidylglycerophosphatase A -
  CJ010_RS03455 (CJ010_03420) thiL 719303..720298 (-) 996 WP_141016750.1 thiamine-phosphate kinase -
  CJ010_RS03460 (CJ010_03425) - 720566..720847 (-) 282 WP_141016751.1 RnfH family protein -

Sequence

Protein


Download         Length: 485 a.a.        Molecular weight: 53987.82 Da        Isoelectric Point: 4.8983

>NTDB_id=243709 CJ010_RS03425 WP_141016744.1 714446..715903(-) (rpoN/rpoN1) [Azoarcus sp. DD4]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNTEIERFLLENPMLEREEPDGGHDFQASIAPSGATQSTSTGDGPGDA
APEHEPRSENSDSASFEEGMDWSGSGSGSSGNRDDDDDVDFQEFQAAGISLRDHLDQQVALSPLSDRDRSLVRFLIEALD
DDGYLHQELEELLELLPPELDVELDDLSIALRHVQSLEPAGIGARTPQECLALQLQAMPANPVRNLALEIVSQHLELLAE
RNFAKLKKLTGCDDDALRAAHALICRLDPHPGAQHSVEETRYVLPDVVVRKQRGRWTVQLNPEAMPRLRINQLYASILQQ
NRGQGGGLTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKFMA
TPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDARIAELLGQQGIVVARRTIAKYRESLNIPPVS
LRKTI

Nucleotide


Download         Length: 1458 bp        

>NTDB_id=243709 CJ010_RS03425 WP_141016744.1 714446..715903(-) (rpoN/rpoN1) [Azoarcus sp. DD4]
ATGAAGCCCACGCTACAACTCAAGCTCTCCCAGCACCTCACGCTGACGCCGCAGCTGCAGCAGTCCATCAAGCTGCTGCA
GCTCTCCACGCTGGAGCTCAACACCGAGATCGAGCGCTTCCTGCTCGAGAACCCGATGCTGGAGCGCGAGGAGCCCGACG
GCGGTCACGATTTCCAGGCTTCGATCGCGCCCTCCGGTGCCACCCAGAGCACGTCGACCGGCGATGGCCCCGGCGATGCG
GCTCCCGAGCACGAACCACGCTCCGAGAACAGCGACTCCGCCAGCTTCGAGGAAGGCATGGACTGGTCGGGCAGCGGCAG
CGGTTCGAGCGGCAACCGCGACGATGACGACGACGTCGACTTCCAGGAATTCCAGGCTGCCGGCATCTCGCTGCGCGACC
ATCTCGACCAGCAAGTCGCCCTGTCGCCGCTGTCCGACCGTGATCGCTCCCTGGTGCGCTTTCTGATCGAGGCACTGGAC
GACGACGGCTACCTACACCAGGAGCTCGAAGAACTGCTCGAACTCCTGCCGCCGGAGCTCGACGTCGAACTCGACGACCT
GAGCATCGCATTGCGCCACGTGCAGAGCCTGGAGCCCGCCGGCATAGGCGCACGCACCCCGCAGGAATGCCTGGCGCTGC
AACTGCAGGCGATGCCGGCCAACCCGGTGCGCAACTTGGCGCTGGAAATCGTCAGCCAGCATCTCGAACTGCTCGCCGAG
CGCAATTTCGCCAAGCTCAAGAAGCTCACCGGCTGCGACGACGACGCCCTGCGCGCGGCCCATGCCCTGATCTGCCGCCT
GGACCCGCATCCGGGCGCCCAGCACTCGGTGGAAGAAACCCGCTACGTGCTGCCCGACGTGGTGGTCCGCAAGCAGCGCG
GCCGCTGGACGGTGCAGCTCAACCCCGAGGCGATGCCGCGCCTGCGCATCAACCAGCTCTACGCCAGCATCCTGCAGCAG
AACCGCGGCCAGGGCGGCGGCCTGACCAGCCAGTTGCAGGAGGCGCGCTGGCTGATCAAGAACGTGCAGCAGCGCTTCGA
CACCATCCTGCGCGTCTCCCAGGCCATCGTTGACCAGCAGCGGCAGTTCTTCGATCATGGGGAGGTGGCGATGCGGCCGC
TCACGCTCAGGGAGATCGCGGACCAGCTCGAATTGCACGAATCCACTGTGTCGCGCGTCACCACGCAGAAGTTCATGGCG
ACCCCGCGCGGCGTGTTCGAACTCAAGTATTTCTTCGGCAGCCATGTCGCCACCGACACCGGTGGCGCGGCCTCTTCCAC
GGCGATTCGCGCGCTGATTCGCCAGCTGGTCGATGCCGAGGACAGGAAAAAACCCCTGTCCGACGCCAGGATTGCCGAAT
TATTGGGCCAGCAGGGTATCGTGGTGGCAAGGCGTACCATCGCCAAGTACCGCGAGTCACTCAATATCCCGCCGGTCAGT
CTGCGCAAAACGATCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-483)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(121-314)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y6KMW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

58.052

100

0.602

Multiple sequence alignment