Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   NTHI_RS02305 Genome accession   NC_007146
Coordinates   458867..459406 (+) Length   179 a.a.
NCBI ID   WP_011272006.1    Uniprot ID   Q4QNH6
Organism   Haemophilus influenzae 86-028NP     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453867..464406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHI_RS02285 (NTHI0481) - 454749..455630 (-) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  NTHI_RS02290 (NTHI0482) - 455936..456400 (+) 465 WP_011272003.1 hypothetical protein -
  NTHI_RS02295 (NTHI0483) - 456492..457370 (-) 879 WP_011272004.1 D-alanyl-D-alanine carboxypeptidase family protein -
  NTHI_RS02300 (NTHI0485) - 457616..458767 (+) 1152 WP_042599461.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NTHI_RS02305 (NTHI0486) pilF/pilF2 458867..459406 (+) 540 WP_011272006.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NTHI_RS02310 (NTHI0487) - 459478..460389 (+) 912 WP_011272007.1 RodZ domain-containing protein -
  NTHI_RS02315 (NTHI0488) ispG 460398..461504 (+) 1107 WP_011272008.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NTHI_RS02320 (NTHI0489) hisS 461514..462785 (+) 1272 WP_011272009.1 histidine--tRNA ligase -
  NTHI_RS02325 (NTHI0490) - 462803..463417 (+) 615 WP_011272010.1 YfgM family protein -
  NTHI_RS02330 (NTHI0491) iscX 463468..463662 (-) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  NTHI_RS02335 (NTHI0492) fdx 463662..464003 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20554.24 Da        Isoelectric Point: 8.9421

>NTDB_id=24368 NTHI_RS02305 WP_011272006.1 458867..459406(+) (pilF/pilF2) [Haemophilus influenzae 86-028NP]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQT
LEKLRQINGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=24368 NTHI_RS02305 WP_011272006.1 458867..459406(+) (pilF/pilF2) [Haemophilus influenzae 86-028NP]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCAATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.089

100

0.961
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment