Detailed information NTG0243322

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CGQ00_RS08740
• Genome accession: NZ_CP022908
• Coordinates: 1681373..1681870 (-)
• Length: 165 a.a.
• NCBI ID: WP_240316779.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain 545
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1676373..1686870

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CGQ00_RS08705 (CGQ00_01612)	gcvPA	1676668..1678014 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
CGQ00_RS08710 (CGQ00_01613)	gcvT	1678034..1679125 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
CGQ00_RS08715 (CGQ00_01614)	-	1679284..1679808 (-)	525	WP_001015117.1	shikimate kinase	-
CGQ00_RS08720	-	1679798..1679944 (-)	147	WP_001789879.1	hypothetical protein	-
CGQ00_RS15975	-	1680041..1680118 (-)	78	Protein_1643	type IV pilus minor pilin ComGF family protein	-
CGQ00_RS08735 (CGQ00_01615)	-	1680217..1681389 (+)	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
CGQ00_RS08740 (CGQ00_01616)	comGF	1681373..1681870 (-)	498	WP_240316779.1	competence type IV pilus minor pilin ComGF	Machinery gene
CGQ00_RS08745 (CGQ00_01617)	comGE	1681788..1682087 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
CGQ00_RS08750 (CGQ00_01618)	comGD	1682074..1682520 (-)	447	WP_001790850.1	competence type IV pilus minor pilin ComGD	Machinery gene
CGQ00_RS08755 (CGQ00_01619)	comGC	1682498..1682809 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
CGQ00_RS08760 (CGQ00_01620)	comGB	1682823..1683893 (-)	1071	WP_000775708.1	competence type IV pilus assembly protein ComGB	Machinery gene
CGQ00_RS08765 (CGQ00_01621)	comGA	1683865..1684839 (-)	975	WP_000697228.1	competence type IV pilus ATPase ComGA	Machinery gene
CGQ00_RS08770 (CGQ00_01622)	-	1684891..1685514 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
CGQ00_RS08775 (CGQ00_01623)	-	1685511..1685840 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
CGQ00_RS08780 (CGQ00_01624)	-	1685840..1686826 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19354.05 Da
• Isoelectric Point: 10.0574

>NTDB_id=243322 CGQ00_RS08740 WP_240316779.1 1681373..1681870(-) (comGF) [Staphylococcus aureus strain 545]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSVKSRLLCKMLYNVFTI
STYQN

Nucleotide

• Length: 498 bp

>NTDB_id=243322 CGQ00_RS08740 WP_240316779.1 1681373..1681870(-) (comGF) [Staphylococcus aureus strain 545]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCAGTCAAGTCCAGACTCCTGTGTAAAATGCTATACAATGTTTTTACCATT
TCTACTTATCAAAATTGA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (67-140)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus MW2	91.463	99.394	0.909
comGF	Staphylococcus aureus N315	95.862	87.879	0.842