Detailed information NTG0243280

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: CGP99_RS08400
• Genome accession: NZ_CP022906
• Coordinates: 1637639..1638136 (-)
• Length: 165 a.a.
• NCBI ID: WP_240316779.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain 546
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	1636483..1637655	1637639..1638136	flank	-16

Gene organization within MGE regions

Location: 1636483..1638136

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CGP99_RS08395 (CGP99_01521)	-	1636483..1637655 (+)	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
CGP99_RS08400 (CGP99_01522)	comGF	1637639..1638136 (-)	498	WP_240316779.1	competence type IV pilus minor pilin ComGF	Machinery gene

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19354.05 Da
• Isoelectric Point: 10.0574

>NTDB_id=243280 CGP99_RS08400 WP_240316779.1 1637639..1638136(-) (comGF) [Staphylococcus aureus strain 546]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSVKSRLLCKMLYNVFTI
STYQN

Nucleotide

• Length: 498 bp

>NTDB_id=243280 CGP99_RS08400 WP_240316779.1 1637639..1638136(-) (comGF) [Staphylococcus aureus strain 546]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCAGTCAAGTCCAGACTCCTGTGTAAAATGCTATACAATGTTTTTACCATT
TCTACTTATCAAAATTGA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (67-140)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus MW2	91.463	99.394	0.909
comGF	Staphylococcus aureus N315	95.862	87.879	0.842