Detailed information NTG0242297

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CJO97_RS02855
• Genome accession: NZ_CP022796
• Coordinates: 594854..595735 (-)
• Length: 293 a.a.
• NCBI ID: WP_075463374.1
• Uniprot ID: -
• Organism: Ralstonia solanacearum strain SL2312
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 589854..600735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CJO97_RS02825 (CJO97_02815)	argJ	590563..591792 (+)	1230	WP_013211398.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
CJO97_RS02830 (CJO97_02820)	-	591808..592692 (+)	885	WP_013211399.1	ATP-binding protein	-
CJO97_RS02835 (CJO97_02825)	-	592670..593131 (+)	462	WP_075463372.1	NUDIX domain-containing protein	-
CJO97_RS02840 (CJO97_02830)	-	593124..593339 (-)	216	WP_013211401.1	DNA gyrase inhibitor YacG	-
CJO97_RS02845 (CJO97_02835)	zapD	593350..594108 (-)	759	WP_013211402.1	cell division protein ZapD	-
CJO97_RS02850 (CJO97_02840)	coaE	594226..594840 (-)	615	WP_075463373.1	dephospho-CoA kinase	-
CJO97_RS02855 (CJO97_02845)	pilD	594854..595735 (-)	882	WP_075463374.1	prepilin peptidase	Machinery gene
CJO97_RS02860 (CJO97_02850)	-	595738..597003 (-)	1266	WP_118882585.1	type II secretion system F family protein	-
CJO97_RS02865 (CJO97_02855)	pilF	597048..598775 (-)	1728	WP_118882586.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CJO97_RS02870 (CJO97_02860)	-	599023..600300 (-)	1278	WP_075463377.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31667.69 Da
• Isoelectric Point: 8.0336

>NTDB_id=242297 CJO97_RS02855 WP_075463374.1 594854..595735(-) (pilD) [Ralstonia solanacearum strain SL2312]
MSGMFVIPTLAQLPAWFVIGSAALLGLLVGSFLNVVIHRLPRMIEREEANYIAELRDEPLPHPEPYNLVIPRSACPSCGH
QIKAVENIPVISWLALRGRCSACKAPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWTIYWAYKLMRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGAVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=242297 CJO97_RS02855 WP_075463374.1 594854..595735(-) (pilD) [Ralstonia solanacearum strain SL2312]
ATGTCCGGGATGTTTGTCATTCCCACGCTGGCGCAGTTGCCGGCGTGGTTCGTGATCGGCTCGGCGGCGCTGCTGGGCCT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGCGAAGAAGCCAACTACATCGCCG
AGCTGCGCGACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCATACCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGATCAGCTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGGCGCCCAT
TTCCTGGCGCTACCCTGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGTGCCTGCCTGTGGCATTTCGGGCCGACCTGGG
TGGCGGTGGCCTCGGCCGCGCTGTTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAACTGCTGCCCGAT
GCCATTACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAATCTGTTCGACATGTTCGCGCGGCTGCCGGATGCGGTGAT
CGGCGCGATGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTACAAGCTGATGCGCGGCCGGGAAGGCATGGGCT
ACGGCGATTTCAAGCTGATGGCCGCGCTGGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTACTGCTGTCGTCG
GTGGTCGGTCTGGTGTTCGGGGCGGTGCGCATCGCGCGCGGCGCCAAGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTCATCGCCCTGCTGGCCGGCCCGCAGCTGGTGGCATTGACGGGGCTCGCGCCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.208	90.444	0.481
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	46.241	90.785	0.42
pilD	Acinetobacter baumannii D1279779	46.241	90.785	0.42