Detailed information NTG0242257

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CJO86_RS02960
• Genome accession: NZ_CP022776
• Coordinates: 608964..609845 (-)
• Length: 293 a.a.
• NCBI ID: WP_075463374.1
• Uniprot ID: -
• Organism: Ralstonia solanacearum strain T11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 603964..614845

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CJO86_RS02930 (CJO86_02910)	argJ	604672..605901 (+)	1230	WP_075463371.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
CJO86_RS02935 (CJO86_02915)	-	605917..606801 (+)	885	WP_013211399.1	ATP-binding protein	-
CJO86_RS02940 (CJO86_02920)	-	606779..607240 (+)	462	WP_075463372.1	NUDIX domain-containing protein	-
CJO86_RS02945 (CJO86_02925)	-	607233..607448 (-)	216	WP_013211401.1	DNA gyrase inhibitor YacG	-
CJO86_RS02950 (CJO86_02930)	zapD	607460..608218 (-)	759	WP_013211402.1	cell division protein ZapD	-
CJO86_RS02955 (CJO86_02935)	coaE	608336..608950 (-)	615	WP_075463373.1	dephospho-CoA kinase	-
CJO86_RS02960 (CJO86_02940)	pilD	608964..609845 (-)	882	WP_075463374.1	prepilin peptidase	Machinery gene
CJO86_RS02965 (CJO86_02945)	-	609848..611113 (-)	1266	WP_075463375.1	type II secretion system F family protein	-
CJO86_RS02970 (CJO86_02950)	pilF	611158..612885 (-)	1728	WP_075463376.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CJO86_RS02975 (CJO86_02955)	-	613133..614410 (-)	1278	WP_075463377.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31667.69 Da
• Isoelectric Point: 8.0336

>NTDB_id=242257 CJO86_RS02960 WP_075463374.1 608964..609845(-) (pilD) [Ralstonia solanacearum strain T11]
MSGMFVIPTLAQLPAWFVIGSAALLGLLVGSFLNVVIHRLPRMIEREEANYIAELRDEPLPHPEPYNLVIPRSACPSCGH
QIKAVENIPVISWLALRGRCSACKAPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWTIYWAYKLMRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGAVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=242257 CJO86_RS02960 WP_075463374.1 608964..609845(-) (pilD) [Ralstonia solanacearum strain T11]
ATGTCCGGGATGTTTGTCATTCCCACGCTGGCGCAGTTGCCGGCGTGGTTCGTGATCGGCTCGGCGGCGCTGCTGGGCCT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCGACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCATACCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGATCAGCTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGGCGCCCAT
TTCCTGGCGCTACCCTGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGTGCCTGCCTGTGGCATTTCGGGCCGACCTGGG
TGGCGGTGGCCTCGGCCGCGCTGTTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAACTGCTGCCCGAT
GCCATTACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAATCTGTTCGACATGTTCGCGCGGCTGCCGGATGCGGTGAT
CGGCGCGATGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTACAAGCTGATGCGCGGCCGGGAAGGCATGGGCT
ACGGCGATTTCAAGCTGATGGCCGCGCTGGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTACTGCTGTCGTCG
GTGGTCGGTCTGGTGTTCGGGGCGGTGCGCATCGCGCGCGGCGCCAAGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTCATCGCCCTGCTGGCCGGCCCGCAGCTGGTGGCATTGACGGGGCTCGCGCCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.208	90.444	0.481
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	46.241	90.785	0.42
pilD	Acinetobacter baumannii D1279779	46.241	90.785	0.42