Detailed information NTG0242174

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CJO78_RS03165
• Genome accession: NZ_CP022761
• Coordinates: 660146..661027 (-)
• Length: 293 a.a.
• NCBI ID: WP_075463374.1
• Uniprot ID: -
• Organism: Ralstonia solanacearum strain T95
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 655146..666027

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CJO78_RS03135 (CJO78_03110)	argJ	655854..657083 (+)	1230	WP_075463371.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
CJO78_RS03140 (CJO78_03115)	-	657099..657983 (+)	885	WP_013211399.1	ATP-binding protein	-
CJO78_RS03145 (CJO78_03120)	-	657961..658422 (+)	462	WP_075463372.1	NUDIX domain-containing protein	-
CJO78_RS03150 (CJO78_03125)	-	658415..658630 (-)	216	WP_013211401.1	DNA gyrase inhibitor YacG	-
CJO78_RS03155 (CJO78_03130)	zapD	658642..659400 (-)	759	WP_013211402.1	cell division protein ZapD	-
CJO78_RS03160 (CJO78_03135)	coaE	659518..660132 (-)	615	WP_075463373.1	dephospho-CoA kinase	-
CJO78_RS03165 (CJO78_03140)	pilD	660146..661027 (-)	882	WP_075463374.1	prepilin peptidase	Machinery gene
CJO78_RS03170 (CJO78_03145)	-	661030..662295 (-)	1266	WP_075463375.1	type II secretion system F family protein	-
CJO78_RS03175 (CJO78_03150)	pilF	662340..664067 (-)	1728	WP_075463376.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CJO78_RS03180 (CJO78_03155)	-	664315..665592 (-)	1278	WP_075463377.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31667.69 Da
• Isoelectric Point: 8.0336

>NTDB_id=242174 CJO78_RS03165 WP_075463374.1 660146..661027(-) (pilD) [Ralstonia solanacearum strain T95]
MSGMFVIPTLAQLPAWFVIGSAALLGLLVGSFLNVVIHRLPRMIEREEANYIAELRDEPLPHPEPYNLVIPRSACPSCGH
QIKAVENIPVISWLALRGRCSACKAPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWTIYWAYKLMRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGAVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=242174 CJO78_RS03165 WP_075463374.1 660146..661027(-) (pilD) [Ralstonia solanacearum strain T95]
ATGTCCGGGATGTTTGTCATTCCCACGCTGGCGCAGTTGCCGGCGTGGTTCGTGATCGGCTCGGCGGCGCTGCTGGGCCT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCGACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCATACCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGATCAGCTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGGCGCCCAT
TTCCTGGCGCTACCCTGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGTGCCTGCCTGTGGCATTTCGGGCCGACCTGGG
TGGCGGTGGCCTCGGCCGCGCTGTTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAACTGCTGCCCGAT
GCCATTACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAATCTGTTCGACATGTTCGCGCGGCTGCCGGATGCGGTGAT
CGGCGCGATGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTACAAGCTGATGCGCGGCCGGGAAGGCATGGGCT
ACGGCGATTTCAAGCTGATGGCCGCGCTGGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTACTGCTGTCGTCG
GTGGTCGGTCTGGTGTTCGGGGCGGTGCGCATCGCGCGCGGCGCCAAGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTCATCGCCCTGCTGGCCGGCCCGCAGCTGGTGGCATTGACGGGGCTCGCGCCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.208	90.444	0.481
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	46.241	90.785	0.42
pilD	Acinetobacter baumannii D1279779	46.241	90.785	0.42