Detailed information NTG0242153

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CJO77_RS02870
• Genome accession: NZ_CP022759
• Coordinates: 604161..605042 (-)
• Length: 293 a.a.
• NCBI ID: WP_048817321.1
• Uniprot ID: A0AAD0S7D3
• Organism: Ralstonia solanacearum strain T98
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 599161..610042

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CJO77_RS02840 (CJO77_02835)	argJ	599870..601099 (+)	1230	WP_118869166.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
CJO77_RS02845 (CJO77_02840)	-	601115..601999 (+)	885	WP_118869167.1	ATP-binding protein	-
CJO77_RS02850 (CJO77_02845)	-	601977..602438 (+)	462	WP_118869168.1	NUDIX domain-containing protein	-
CJO77_RS02855 (CJO77_02850)	-	602431..602646 (-)	216	WP_013211401.1	DNA gyrase inhibitor YacG	-
CJO77_RS02860 (CJO77_02855)	zapD	602658..603416 (-)	759	WP_013211402.1	cell division protein ZapD	-
CJO77_RS02865 (CJO77_02860)	coaE	603534..604148 (-)	615	WP_013211403.1	dephospho-CoA kinase	-
CJO77_RS02870 (CJO77_02865)	pilD	604161..605042 (-)	882	WP_048817321.1	prepilin peptidase	Machinery gene
CJO77_RS02875 (CJO77_02870)	-	605045..606310 (-)	1266	WP_075463375.1	type II secretion system F family protein	-
CJO77_RS02880 (CJO77_02875)	pilF	606355..608082 (-)	1728	WP_013211406.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CJO77_RS02885 (CJO77_02880)	-	608330..609607 (-)	1278	WP_013211407.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31635.63 Da
• Isoelectric Point: 8.0336

>NTDB_id=242153 CJO77_RS02870 WP_048817321.1 604161..605042(-) (pilD) [Ralstonia solanacearum strain T98]
MSGMFVIPTLAQLPAWFVIGSAALLGLLVGSFLNVVIHRLPRMIEREEANYIAELRDEPLPHPEPYNLVIPRSACPSCGH
QIKAVENIPVISWLALRGRCSACKAPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLVAGSMIDADTQLLPD
AITQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWTIYWAYKLLRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGAVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=242153 CJO77_RS02870 WP_048817321.1 604161..605042(-) (pilD) [Ralstonia solanacearum strain T98]
ATGTCCGGGATGTTTGTCATTCCCACGCTGGCGCAGTTGCCGGCGTGGTTCGTGATCGGCTCGGCGGCGCTGCTGGGCCT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCGACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCATACCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGATCAGCTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGGCGCCCAT
TTCCTGGCGCTACCCTGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGTGCCTGCCTGTGGCATTTCGGGCCGACCTGGG
TAGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGGTCGCCGGCTCGATGATCGACGCCGACACGCAACTGCTGCCTGAT
GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAATCTGTTCGACATGTTCGCGCGGCTGCCGGATGCGGTGAT
CGGTGCGATGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTACAAGCTGCTGCGCGGCCGGGAAGGCATGGGCT
ACGGCGATTTCAAGCTGATGGCCGCGCTGGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCGTCG
GTGGTCGGTCTGGTGTTCGGGGCGGTGCGCATCGCGCGCGGCGCCAAGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTCATCGCCCTGCTGGCCGGCCCGCAGCTGGTGGCATTGACGGGGCTCGCGCCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

• Type IV leader peptidase family (Peptidase_A24) (140-247)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.208	90.444	0.481
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	45.865	90.785	0.416
pilD	Acinetobacter baumannii D1279779	45.865	90.785	0.416