Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CCZ37_RS11730 Genome accession   NZ_CP022741
Coordinates   2518604..2519614 (-) Length   336 a.a.
NCBI ID   WP_094500584.1    Uniprot ID   A0A223N0B8
Organism   Vibrio qinghaiensis strain Q67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2513604..2524614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCZ37_RS11705 (CCZ37_11690) aroK 2514456..2514977 (-) 522 WP_010317691.1 shikimate kinase AroK -
  CCZ37_RS11710 (CCZ37_11695) pilQ 2515164..2516894 (-) 1731 WP_094500581.1 type IV pilus secretin PilQ family protein Machinery gene
  CCZ37_RS11715 (CCZ37_11700) pilP 2516918..2517439 (-) 522 WP_010317687.1 pilus assembly protein PilP Machinery gene
  CCZ37_RS11720 (CCZ37_11705) pilO 2517426..2518019 (-) 594 WP_094500582.1 type 4a pilus biogenesis protein PilO Machinery gene
  CCZ37_RS11725 (CCZ37_11710) pilN 2518012..2518614 (-) 603 WP_094500583.1 PilN domain-containing protein Machinery gene
  CCZ37_RS11730 (CCZ37_11715) pilM 2518604..2519614 (-) 1011 WP_094500584.1 type IV pilus assembly protein PilM Machinery gene
  CCZ37_RS11735 (CCZ37_11720) - 2519764..2522262 (+) 2499 WP_010317677.1 PBP1A family penicillin-binding protein -
  CCZ37_RS11740 (CCZ37_11725) oxyR 2522299..2523189 (-) 891 WP_094500585.1 DNA-binding transcriptional regulator OxyR -
  CCZ37_RS11745 (CCZ37_11730) - 2523337..2524065 (+) 729 WP_010317674.1 redoxin family protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37350.99 Da        Isoelectric Point: 7.2362

>NTDB_id=242076 CCZ37_RS11730 WP_094500584.1 2518604..2519614(-) (pilM) [Vibrio qinghaiensis strain Q67]
MGKSLVTGIDIGHYSIKAVVLKPAGDSYTLIGCKELLITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSHKVAISVPDNA
VISKVLQIDSDLQAPEKEFAIYQAFSHQSPFPMEELSLDFVKVMKKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLNIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDRVMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLLFNNKLAKRQQGELSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=242076 CCZ37_RS11730 WP_094500584.1 2518604..2519614(-) (pilM) [Vibrio qinghaiensis strain Q67]
ATGGGTAAATCATTGGTTACGGGTATAGATATTGGCCACTATAGTATTAAAGCGGTGGTATTAAAGCCAGCAGGTGATTC
ATATACACTGATTGGCTGTAAAGAACTACTTATTACAGAGGATATATTCTCTGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCACAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCCTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATGGAAGAGCTGAGTCTCGATTTCGTCAAAGTGATGAAAAAAAGCGTAAGTCGCAGTAGTACCG
TAAGTTATCAAGTTTACGCCACCCGCAAAGACGTCGTGGAAAGCCGCGCGTTAGCGGCGCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAACATTTGGCAGTTGGCGAGCAGGGCACAGCATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAAATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACCGTGTGATGTCGGGCACCAATGCCGAATTAGCTGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGCAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGCGCAGATACTCCGATGTTGGCGCAAGAGATCTCGCGGCGTCTCAAGCTTGAGTGCGAGATTCTCG
ATCCTTTCCTTCTGTTTAACAATAAGTTAGCGAAACGCCAGCAGGGTGAGCTGTCTGGGCAGAGTTTTAGCACCGCAGCG
GGTCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223N0B8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

65.68

100

0.661
  pilM Vibrio campbellii strain DS40M4

52.083

100

0.521

Multiple sequence alignment