Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BaGK_RS06145 Genome accession   NZ_CP022653
Coordinates   1242546..1246250 (+) Length   1234 a.a.
NCBI ID   WP_094231770.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain GQJK17     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1237546..1251250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BaGK_RS06130 (BaGK_06130) - 1237993..1238319 (-) 327 WP_094231768.1 four-helix bundle copper-binding protein -
  BaGK_RS06135 (BaGK_06135) - 1238416..1238853 (-) 438 WP_003327683.1 ferritin family protein -
  BaGK_RS06140 (BaGK_06140) addB 1239059..1242559 (+) 3501 WP_094231769.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BaGK_RS06145 (BaGK_06145) addA 1242546..1246250 (+) 3705 WP_094231770.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BaGK_RS06150 (BaGK_06150) sbcD 1246321..1247499 (+) 1179 WP_094231771.1 exonuclease subunit SbcD -
  BaGK_RS06155 (BaGK_06155) sbcC 1247496..1250888 (+) 3393 WP_094231772.1 exonuclease subunit SbcC -
  BaGK_RS06160 (BaGK_06160) - 1250905..1251207 (+) 303 WP_094231773.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141492.05 Da        Isoelectric Point: 5.4163

>NTDB_id=241218 BaGK_RS06145 WP_094231770.1 1242546..1246250(+) (addA) [Bacillus atrophaeus strain GQJK17]
MQIPKPADSTWTDDQWNAIVSSGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLRKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPKTWLEGFVHLYDASEKSTIEELPFYPYMKEDIAMVLSAVKEKLQRA
LELTKVPGGPAPRADNFLDDLAQTEDLIAHSADFTELYKRVPAVSFKRAKAVKGDEFDQALTEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLSDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAVNESGERKPSEAAAYYQEQ
FQEVLVDEYQDTNLVQEAILQLVTSGPEASGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTETGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKVGEVDYDEQAELKLGASYPPNDQTETELLLIDRAEEKEASEETEELETVQLEAKAIAKQIR
KLVSSPYKVYDGKSKTHSNIRYRDIVILLRSMPWAPQIMEELKAQGIPVYANLTSGYFEAVEVSVAISALKVIDNPYQDI
PLASVLRSPIVGADENELSLIRMHNKKAPFYEAMKDYLAGGDRNDELYQKLNTFFELLQKWRSFSKNHSVSELIWEVYRD
SKYMDYVGGMPGGKQRQANLRVLYDRSRQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQASASQPDWLLPDFERYQAKTYLDFIGPALIRHRDMGTDALQGLASQADISEHPARFNIQMVSAQD
LLDDDLEERQEEKSGRLDAIRRGEPVSGSFEFEEQARQRLSWRYPYHDVTKIRTKQTVSEIKRKKEYEDEYSGRSLIRPA
DGNMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHAPSKEEAERIVKRLYEKELLTEDQQAVIDLEEIVQFFHTEIGE
KLIGAKWINREVPFSMALSAQDAFLDTESKDEPILVQGIIDCLYETDEGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIELYTKAVEQITKTNVKGRALYFFDGGHVLNV

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=241218 BaGK_RS06145 WP_094231770.1 1242546..1246250(+) (addA) [Bacillus atrophaeus strain GQJK17]
ATGCAAATTCCTAAACCGGCAGACAGCACCTGGACAGATGACCAGTGGAACGCCATTGTCTCATCCGGACAGGATATTCT
CGTTGCAGCGGCCGCTGGATCTGGAAAAACCGCTGTGCTTGTTGAGAGAATGATCCGAAAAATTACCGCGGAGGAAAATC
CGATAGATGTGGACCGTCTTTTGGTCGTGACATTTACGAACGCCTCTGCGGCTGAAATGAAGCACCGGATCGCGGAGGCT
TTGGAGAAAGAGCTCGTGAAACGTCCCGGCTCTCTTCACATCAGGCGGCAGCTGTCTCTGTTGAACCGGGCCAGCATTTC
AACCCTTCATTCGTTCTGCTTGCAGGTGCTCAGAAAATATTATTATCTCATTGATCTTGATCCGGGCTTTCGGATTGCCG
ATCAGACAGAAGGCGAGCTGCTCGGGGACGAGGTCCTTGATGAACTGTTTGAAGACGAATACGCGAAGGGTGAAAAAGCG
TTTTTCGAACTGGTGGACCGGTATACGACAGACCGGCACGACTTAGACCTTCAGCACCTAGTGAAACGGGTGTATGAGTT
TTCCCGTTCCCATCCCGATCCAAAAACGTGGCTGGAAGGTTTCGTTCATCTTTACGATGCCTCAGAAAAAAGTACGATTG
AAGAACTGCCGTTTTATCCATATATGAAAGAAGACATTGCGATGGTGCTGAGTGCTGTGAAAGAGAAGCTTCAGCGGGCG
CTTGAGCTGACAAAAGTGCCGGGCGGCCCTGCGCCGCGCGCTGATAATTTTCTGGATGATCTTGCACAGACAGAGGATCT
GATCGCACATTCGGCGGATTTCACTGAATTATACAAGCGGGTTCCCGCAGTCTCCTTTAAGCGTGCCAAAGCGGTCAAAG
GAGATGAATTTGACCAGGCGCTCACAGAAGAAGCGACGGATTTACGGAACGGCGCGAAAAAACTGCTGGAAAAACTAAAA
ACGGATTATTTCACAAGAAGCCCCGAGCAGCATCTGAAGAGCCTGTCGGATATGAAGCCTGTGATTGAAACGCTTGTCCA
GCTTGTCATCAGCTACGGAAAACGGTTTGAAGCCGCCAAACAAGAGAAATCAATTATTGATTTCTCTGACTTGGAGCATT
ACTGCTTGTCTATCCTGACAGCCGTGAATGAGTCGGGGGAGCGCAAGCCGAGTGAGGCGGCGGCTTACTATCAAGAGCAG
TTCCAAGAGGTGCTGGTTGACGAATATCAAGATACGAATCTTGTGCAGGAAGCCATACTGCAGCTTGTCACAAGCGGACC
TGAAGCTTCAGGAAACTTGTTTATGGTAGGGGACGTCAAACAGTCCATCTACCGATTCAGGCTTGCCGAACCGCTTCTTT
TCCTTTCTAAATATAAACGATTTACCGAAACGGGAGAGGGGACAGGCCGGAAAATTGATTTGAACAAAAACTTTCGAAGC
CGGGCGGATATTTTAGACAGCACGAACTTTCTGTTTAAGCAGCTCATGGGCGGCAAGGTCGGCGAGGTTGATTATGATGA
GCAGGCGGAATTGAAGCTAGGAGCCTCTTACCCTCCGAATGACCAGACTGAAACGGAGCTGTTGCTGATTGACAGAGCGG
AGGAAAAAGAGGCGAGTGAAGAAACAGAAGAGCTCGAAACCGTTCAGCTTGAAGCAAAAGCGATTGCAAAGCAGATTCGA
AAGCTCGTATCCTCTCCTTACAAAGTCTATGATGGAAAATCGAAAACACACAGCAATATCCGCTACCGCGATATCGTGAT
TTTACTGCGTTCCATGCCGTGGGCGCCGCAAATCATGGAAGAACTGAAAGCTCAAGGCATACCCGTCTATGCCAATTTGA
CATCTGGCTATTTTGAAGCGGTCGAGGTGTCTGTTGCGATTTCCGCTTTAAAAGTGATTGATAATCCGTACCAAGATATT
CCTCTCGCTTCCGTGCTGCGCTCGCCGATTGTCGGCGCGGATGAAAATGAACTTTCGCTGATTCGGATGCATAATAAAAA
AGCGCCTTTCTATGAAGCGATGAAGGACTATCTTGCAGGCGGGGACCGAAATGACGAGCTATATCAAAAGCTGAACACGT
TTTTTGAGCTTTTGCAAAAATGGCGCTCTTTTTCGAAAAACCATTCAGTTTCCGAGCTGATATGGGAAGTTTATCGTGAC
AGCAAATATATGGACTATGTCGGCGGTATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGTTCTCTATGACCGGTC
CCGCCAGTATGAATCGACGGCATTTCGCGGTTTATTTCGTTTCTTGCGGTTTATAGAACGTATGCAGGAACGGGGCGATG
ACCTCGGGACAGCGAGAGCGCTCAGCGAGCAGGAGGACGTGGTCCGCCTGATGACGATTCACAGCAGCAAAGGGCTTGAA
TTCCCTGTCGTATTCGCTGCGGGACTTGGACGGAATTTTAATATGATGGATTTGAATAAATCATATCTGCTGGACAAAGA
GCTTGGATTCGGCACAAAGTTTATTCACCCCGAATGGCGCATCAGCTATCCGACACTTCCGCTCATCGCCATGAAGAAAA
AAATGCGCCGGGAGCTGCTGTCAGAAGAGCTGCGTGTGCTATACGTGGCTTTAACAAGAGCGAAGGAAAAGCTGTTTCTT
GTCGGTTCCTGCAAGAATCGTGAGAAGCAGCTGGCGAAATGGCAGGCTTCAGCGTCTCAGCCAGACTGGCTTCTCCCGGA
TTTTGAGCGGTATCAGGCGAAAACGTATTTGGATTTCATCGGTCCCGCATTGATTCGTCACAGAGACATGGGGACAGATG
CTTTACAAGGGCTTGCGAGTCAAGCTGACATATCAGAACACCCTGCCCGGTTTAACATCCAAATGGTCAGCGCCCAAGAT
TTATTGGATGATGATCTGGAAGAAAGACAAGAGGAAAAGAGCGGCCGTCTTGACGCCATCCGCCGCGGTGAGCCTGTGTC
CGGTTCGTTTGAATTCGAAGAACAAGCGCGGCAACGCCTGTCATGGCGATACCCTTATCACGACGTGACAAAAATTCGCA
CGAAGCAGACTGTTTCCGAGATCAAGAGAAAGAAAGAATACGAGGACGAATACAGCGGCCGCTCTCTTATCAGACCTGCT
GACGGAAACATGTTGTACAGGCGCCCTGCGTTTATGATGAAAAAAGGGCTGACTGCGGCTGAAAAAGGAACGGCGATGCA
CACCGTTATGCAGCATATCCCGCTGACACATGCACCGTCAAAAGAGGAAGCGGAACGTATTGTCAAACGTCTTTATGAAA
AAGAGCTGCTCACCGAAGACCAGCAAGCCGTTATTGACCTAGAGGAAATCGTGCAATTTTTCCATACTGAAATCGGCGAA
AAGCTGATCGGCGCAAAATGGATTAACCGCGAAGTGCCGTTTAGCATGGCGCTTTCAGCACAAGATGCGTTTCTGGACAC
TGAATCTAAAGATGAGCCTATATTGGTGCAAGGGATTATTGACTGTCTGTATGAGACCGATGAAGGGCTTTACTTGCTGG
ATTACAAAACGGACCGAATCGAAGGGAAATTCCAGCACGGATTTGAGGGGGCGGCTCCTATTCTTAAAAAACGGTACGAA
ACTCAAATCGAGTTATACACAAAAGCTGTAGAACAAATAACGAAAACCAATGTGAAGGGACGTGCACTCTACTTCTTCGA
TGGAGGACACGTTTTGAACGTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

86.629

100

0.866

Multiple sequence alignment