Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BaGK_RS06140 Genome accession   NZ_CP022653
Coordinates   1239059..1242559 (+) Length   1166 a.a.
NCBI ID   WP_094231769.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain GQJK17     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1234059..1247559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BaGK_RS06115 (BaGK_06115) - 1234458..1235945 (+) 1488 WP_420885127.1 monooxygenase -
  BaGK_RS06120 (BaGK_06120) - 1236029..1236559 (+) 531 WP_003327686.1 MarR family transcriptional regulator -
  BaGK_RS06125 (BaGK_06125) - 1236611..1237759 (-) 1149 WP_010788164.1 AbrB family transcriptional regulator -
  BaGK_RS06130 (BaGK_06130) - 1237993..1238319 (-) 327 WP_094231768.1 four-helix bundle copper-binding protein -
  BaGK_RS06135 (BaGK_06135) - 1238416..1238853 (-) 438 WP_003327683.1 ferritin family protein -
  BaGK_RS06140 (BaGK_06140) addB 1239059..1242559 (+) 3501 WP_094231769.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BaGK_RS06145 (BaGK_06145) addA 1242546..1246250 (+) 3705 WP_094231770.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BaGK_RS06150 (BaGK_06150) sbcD 1246321..1247499 (+) 1179 WP_094231771.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134401.17 Da        Isoelectric Point: 5.1709

>NTDB_id=241217 BaGK_RS06140 WP_094231769.1 1239059..1242559(+) (addB) [Bacillus atrophaeus strain GQJK17]
MGAEFLVGRSGSGKTKLVIDSIQDELRRNPFGKPIIFLVPDQMTFLMEYELAKTPGIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTSTGIQMLLRKLIEEHKQEFKVYQKASDKSGFTEQVERMLTEFKRYCLEPEDIRRMIESGTATDYRGERVLTD
KLHDLYILYQQMEKSLENQYLHSEDYLTLLAEQIPYAEDLKGAHVYVDGFYQFTPQEFRVLTQLMAQAEHVTFSLTADKP
SHVQAPDELDLFRMTGKTYYQLHQAASELHLDITNRELTETKRYHQTPELAHLEAMYDARPAVPFLEKQEAFTVMQAANR
RTELEGIAREIHALVRDKGLRYKDMAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLVEFIRSSLDVIKGNWRY
EAVFRCVKTELLFPLDRPKNIIREQVDQLENYCIAYGIKGDRWTRGDRFVYRRFVSLDDDFAQTDQEIEMENMVNDTKDW
IVPPLFRLQKRMKQAKTVQDMAEALYLYLEETDIPLKLDQERQRAEEDGRIIEAQQHNQAWDAVIQLLEEFVEMMGEDEI
SLSLFQQMMETGAESLKFALIPPALDQVFVGNMDLSRMYGTRCTFVIGANDGVLPARPEDDGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSTVVKRLTELFPEHQERLLTNEPEQVSDEEQLLYLVNKS
VAQSYTASQFRLWLREYDISDVWWSAYNVLMNEPDRLNSKKLFSSLFFRNEVKPLERAVSRQLYGEHIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKDQCNKFSYEAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVARVSGILSEHAKASGFAPVGLELGFGGKGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLSEVYYGLALQMLTYLDLSITYSADWLGMKATPAGVLYFHIHDPMIQASVPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEEEFHLLTQHVRRTFQEAGEQITDGRVSIEPYKLKDKTP
CTYCSFKSVCQFDESLEENEYRTLKAEKDHTILDWLKKEGDGDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=241217 BaGK_RS06140 WP_094231769.1 1239059..1242559(+) (addB) [Bacillus atrophaeus strain GQJK17]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGGTCATAGACAGCATTCAAGATGAGCTGCG
CCGGAATCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCTGGGATAGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGTTTGGCCTGGCGTGTCCTGCAGCATACGGGC
GGAATGAACCGGCCGTTTGTCACCAGCACGGGAATCCAAATGCTTTTGAGAAAGCTCATTGAAGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGAACAGGTAGAACGGATGCTGACGGAATTTAAACGGTACT
GCCTTGAGCCTGAAGATATCCGCCGCATGATTGAAAGCGGAACTGCCACAGACTATCGCGGCGAGCGGGTGTTAACCGAT
AAGCTGCATGATTTGTATATTTTATATCAGCAGATGGAAAAGAGCCTTGAAAACCAGTATCTTCATTCAGAGGATTATTT
AACGCTGCTGGCGGAACAGATCCCGTATGCGGAAGATTTGAAAGGGGCGCATGTCTATGTAGACGGCTTTTATCAGTTTA
CTCCGCAGGAATTCAGAGTGCTTACGCAGCTTATGGCACAGGCTGAGCATGTGACGTTTTCACTGACGGCGGACAAGCCG
TCCCATGTGCAGGCGCCTGATGAGCTGGATCTGTTCAGAATGACAGGCAAAACATATTATCAGCTCCATCAAGCAGCATC
GGAGCTTCATTTGGACATCACAAACAGAGAACTGACCGAGACGAAACGATATCACCAAACACCGGAGCTTGCCCATTTAG
AAGCAATGTATGACGCGCGCCCTGCTGTTCCGTTTTTGGAAAAGCAAGAAGCCTTTACCGTGATGCAGGCTGCGAACAGG
CGGACAGAGCTGGAAGGAATTGCTCGTGAAATCCATGCTCTTGTAAGAGATAAGGGATTACGCTACAAGGATATGGCAAT
TCTCGCCCGCCAGCCGGAAGATTATAAGGATATGGTTAAAGAAGTATTTGCAGATTATGAAATTCCTTACTTTATAGATG
GAAAGGCATCCATGCTGAACCATCCGCTTGTTGAGTTTATCCGTTCAAGCCTTGATGTCATAAAGGGAAATTGGAGATAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGTTATTCCCGCTTGACCGTCCGAAGAACATCATAAGGGAGCAGGTCGA
TCAACTGGAAAACTATTGTATCGCTTACGGAATTAAAGGCGACCGATGGACGAGAGGCGACCGTTTTGTTTACAGGCGGT
TTGTTTCACTGGATGATGATTTTGCGCAGACAGATCAAGAGATTGAAATGGAAAACATGGTGAATGACACAAAAGACTGG
ATTGTTCCGCCGCTTTTCCGTCTTCAAAAACGGATGAAACAAGCAAAGACGGTCCAAGACATGGCGGAGGCTCTTTATCT
TTATTTAGAAGAAACGGATATTCCGTTAAAGCTGGACCAAGAAAGGCAGCGCGCGGAAGAGGACGGGAGAATTATTGAAG
CGCAGCAGCATAATCAGGCTTGGGACGCTGTTATACAGCTTCTCGAGGAATTTGTGGAAATGATGGGAGAAGACGAAATT
TCTCTGTCTCTTTTTCAGCAAATGATGGAGACGGGTGCGGAATCGTTAAAATTTGCTCTGATTCCGCCTGCACTCGATCA
AGTCTTTGTCGGCAATATGGATTTATCGAGAATGTACGGTACACGCTGCACCTTTGTTATCGGTGCGAACGACGGCGTAC
TTCCGGCGCGGCCTGAGGATGACGGGGTGCTGTCTGATGACGACAGGGAATGGCTGAAAACCATTGGAGTCGAGCTGTCA
TCCGGAGGGCGCGAACGTCTGCTTGATGAGCACTTTCTCATATATATGGCGCTTTCCAGTCCGTCTGACCGCCTGTATGT
ATCCTACCCGATCGCAGACGCCGAAGGTAAAACGCTTTTGCCGTCAACTGTGGTCAAACGGCTTACAGAACTGTTTCCGG
AGCATCAGGAAAGGCTTTTGACCAATGAACCGGAACAGGTCAGCGACGAGGAACAGCTCTTGTATCTCGTCAATAAAAGC
GTGGCGCAATCGTATACGGCAAGCCAATTCAGGTTGTGGTTAAGGGAGTATGACATCAGTGATGTATGGTGGAGCGCGTA
TAATGTATTGATGAATGAACCGGACAGGCTGAACTCGAAAAAACTTTTCTCCAGCCTGTTTTTCAGAAATGAAGTCAAAC
CGCTTGAACGCGCTGTATCGAGACAGCTGTACGGCGAGCATATTCAGGGAAGTGTGTCCAGAATGGAGACCTTTAACGCT
TGTCCGTTCTCTCATTTTGCGTCCCATGGCTTACATCTGAAAGAACGGCAATTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTCCATTCCAGTCTGAAGCTGATTTCAGACCGGCTTCGTGAACAAAAGCTGGATTGGCGCGATTTAACAAAAG
ACCAATGTAACAAGTTCTCTTACGAGGCGGTGGAGCGGCTCGCGCCGAAGCTCCAAAAAGAAATTCTGCTTAGCTCAAAC
AGACATTATTATGTCAAGGAAAAGCTGCAAAAAATTGTGGCGCGCGTGTCCGGCATTTTAAGCGAGCATGCGAAGGCGAG
CGGGTTTGCACCGGTTGGGCTTGAGCTTGGTTTTGGCGGGAAAGGCCCGCTGCCGCCTCTTACATTCACATTAAAAAACG
GCTGCACGATGGAGCTTGTCGGACGCATTGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGGCTGCTGCTGAGAATAGTG
GATTATAAATCCAGCGACAAGGGTCTTGATTTGTCAGAGGTATATTACGGCCTTGCCCTGCAAATGCTCACGTACCTTGA
TTTATCCATTACGTATTCGGCAGATTGGCTCGGCATGAAGGCTACACCGGCCGGCGTTCTTTATTTCCATATTCACGACC
CGATGATTCAAGCCAGCGTTCCGCTCGGGCTTGATGAAATAGAGCAGGAAATCTTTAAGAAATTTAAGATGAAAGGCCTG
CTGCTCGGCGATCAGGAAGCGATTCGATTGATGGATACGACACTCCAAGAGGGAAGGTCAAATATCATTAATGCCGGTCT
GAAAAAAGACGGTTCATTAAGGTCTGATTCAGCGGCGGTCGGAGAAGAGGAATTTCACCTGCTGACACAGCACGTGCGGC
GGACCTTTCAGGAGGCCGGCGAACAAATTACCGACGGGCGTGTGTCGATTGAGCCGTATAAGCTGAAAGATAAAACACCC
TGCACATACTGCTCATTCAAATCAGTATGCCAGTTCGATGAATCATTGGAAGAAAACGAATATCGCACGCTGAAAGCGGA
AAAGGATCATACCATATTAGATTGGCTGAAAAAGGAGGGAGATGGCGATGCAAATTCCTAA

Domains

Predicted by InterProScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

88.765

100

0.888

Multiple sequence alignment