Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   FORC62_RS00305 Genome accession   NZ_CP022582
Coordinates   59818..61167 (-) Length   449 a.a.
NCBI ID   WP_000815646.1    Uniprot ID   Q9XB96
Organism   Staphylococcus aureus strain FORC_062     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 36958..61167 59818..61167 within 0


Gene organization within MGE regions


Location: 36958..61167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC62_RS00165 (FORC62_0030) mecI 36958..37329 (-) 372 WP_000369211.1 mecA-type methicillin resistance repressor MecI -
  FORC62_RS00170 (FORC62_0031) mecR1 37329..39086 (-) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  FORC62_RS00175 (FORC62_0032) mecA 39183..41192 (+) 2010 WP_114914741.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  FORC62_RS00180 (FORC62_0033) - 41238..41666 (-) 429 WP_000872606.1 MaoC family dehydratase -
  FORC62_RS00185 (FORC62_0034) - 41763..42506 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  FORC62_RS00195 - 43183..43572 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  FORC62_RS00200 (FORC62_0036) - 43608..44282 (+) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  FORC62_RS00205 (FORC62_0037) - 44523..45653 (+) 1131 WP_010922808.1 ROK family transcriptional regulator -
  FORC62_RS00215 (FORC62_0039) cstB 45767..47101 (-) 1335 Protein_36 persulfide dioxygenase-sulfurtransferase CstB -
  FORC62_RS00220 (FORC62_0040) - 47134..48198 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  FORC62_RS00225 (FORC62_0041) cstR 48334..48594 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  FORC62_RS00230 (FORC62_0042) - 48594..49232 (+) 639 Protein_39 sulfite exporter TauE/SafE family protein -
  FORC62_RS15455 - 49283..49345 (-) 63 Protein_40 DNA repair protein RadC -
  FORC62_RS00240 (FORC62_0043) - 49577..50239 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  FORC62_RS15460 - 50631..50690 (+) 60 WP_010959190.1 erythromycin resistance leader peptide -
  FORC62_RS00255 (FORC62_0044) erm(A) 50773..51504 (+) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  FORC62_RS00260 (FORC62_0045) - 51630..52412 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  FORC62_RS00265 (FORC62_0046) - 52563..52940 (-) 378 WP_000361059.1 DUF6262 family protein -
  FORC62_RS00270 (FORC62_0047) - 52947..54839 (-) 1893 WP_001557544.1 site-specific integrase -
  FORC62_RS00275 (FORC62_0048) - 54836..55921 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  FORC62_RS00280 (FORC62_0049) - 56040..56357 (-) 318 WP_000659050.1 JAB domain-containing protein -
  FORC62_RS00285 (FORC62_0050) - 56378..56884 (-) 507 WP_001092169.1 DUF1643 domain-containing protein -
  FORC62_RS00290 (FORC62_0051) - 56902..57213 (-) 312 WP_000700853.1 DUF960 family protein -
  FORC62_RS00295 (FORC62_0052) - 57300..57650 (-) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  FORC62_RS00300 (FORC62_0053) ccrB 58168..59796 (-) 1629 WP_001186602.1 recombinase family protein Machinery gene
  FORC62_RS00305 (FORC62_0054) ccrA 59818..61167 (-) 1350 WP_000815646.1 recombinase family protein Machinery gene

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52607.20 Da        Isoelectric Point: 9.9585

>NTDB_id=240969 FORC62_RS00305 WP_000815646.1 59818..61167(-) (ccrA) [Staphylococcus aureus strain FORC_062]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLTNMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=240969 FORC62_RS00305 WP_000815646.1 59818..61167(-) (ccrA) [Staphylococcus aureus strain FORC_062]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATACAACACTTACGAATATGACCGTTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCTCGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9XB96

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

100

100

1
  ccrA Staphylococcus aureus COL

74.833

100

0.748

Multiple sequence alignment