Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CGU46_RS15335 Genome accession   NZ_CP022525
Coordinates   3204311..3205531 (-) Length   406 a.a.
NCBI ID   WP_023101565.1    Uniprot ID   A0A509JJC1
Organism   Pseudomonas aeruginosa strain Ocean-1175     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3199311..3210531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGU46_RS15290 (CGU46_15290) - 3199492..3199941 (-) 450 WP_021264127.1 MOSC domain-containing protein -
  CGU46_RS15295 (CGU46_15295) - 3199938..3200567 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  CGU46_RS15300 (CGU46_15300) - 3200700..3201125 (+) 426 WP_021264128.1 GNAT family N-acetyltransferase -
  CGU46_RS15305 (CGU46_15305) - 3201122..3201592 (+) 471 WP_003094664.1 hypothetical protein -
  CGU46_RS15310 (CGU46_15310) - 3201589..3202278 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  CGU46_RS15315 (CGU46_15315) - 3202384..3202593 (+) 210 WP_003094660.1 hypothetical protein -
  CGU46_RS15320 (CGU46_15320) yacG 3202630..3202830 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CGU46_RS15325 (CGU46_15325) coaE 3202827..3203438 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  CGU46_RS15330 (CGU46_15330) pilD 3203435..3204307 (-) 873 WP_021264129.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CGU46_RS15335 (CGU46_15335) pilC 3204311..3205531 (-) 1221 WP_023101565.1 type 4a pilus biogenesis protein PilC Machinery gene
  CGU46_RS15340 (CGU46_15340) pilB 3205535..3207235 (-) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CGU46_RS15345 (CGU46_15345) pilA 3207467..3207916 (+) 450 WP_021264133.1 pilin Machinery gene
  CGU46_RS15355 (CGU46_15355) ltrA 3208533..3209954 (+) 1422 WP_011805433.1 group II intron reverse transcriptase/maturase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44346.12 Da        Isoelectric Point: 9.8784

>NTDB_id=240256 CGU46_RS15335 WP_023101565.1 3204311..3205531(-) (pilC) [Pseudomonas aeruginosa strain Ocean-1175]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGVIGFTFK
ELHKRSKKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=240256 CGU46_RS15335 WP_023101565.1 3204311..3205531(-) (pilC) [Pseudomonas aeruginosa strain Ocean-1175]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAGGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGT
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTAATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTCCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCCTTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAATGGTGGCTTGCCGTCATCGTTGGCGTGGGGGTGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCAAAAAAATTCCGCGACACACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCTGCGGTCGCTCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A509JJC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.644

99.507

0.603
  pilC Acinetobacter baylyi ADP1

60.345

100

0.603
  pilC Legionella pneumophila strain ERS1305867

54.545

97.537

0.532
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.365

100

0.424
  pilC Vibrio campbellii strain DS40M4

41.604

98.276

0.409
  pilC Thermus thermophilus HB27

36.908

98.768

0.365

Multiple sequence alignment