Detailed information NTG0000641

Experimentally validated

Overview

• Name: sbcC
• Type: Machinery gene
• Locus tag: BSU_10650
• Genome accession: NC_000964
• Coordinates: 1144749..1148141 (+)
• Length: 1130 a.a.
• NCBI ID: NP_388946.2
• Uniprot ID: O06714
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: homologous recombination Homologous recombination

Function

SbcC plays an important role in the repair of DNA inter-strand cross-links (induced by MMC), most likely through HR, and suggest that NHEJ via Ku serves as a backup DNA repair system.

Genomic Context

Location: 1139749..1153141

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_10630 (BSU10630)	addA	1139807..1143505 (+)	3699	NP_388944.2	ATP-dependent deoxyribonuclease (subunit A)	Machinery gene
BSU_10640 (BSU10640)	sbcD	1143577..1144752 (+)	1176	NP_388945.3	DNA repair exonuclease	-
BSU_10650 (BSU10650)	sbcC	1144749..1148141 (+)	3393	NP_388946.2	DNA ATP-dependent repair enzyme	Machinery gene
BSU_10660 (BSU10660)	hlpB	1148155..1148457 (+)	303	NP_388947.1	HNH nuclease-like essential for DNA repair	-
BSU_10670 (BSU10670)	gerPF	1148494..1148712 (-)	219	NP_388948.2	spore germination protein	-
BSU_10680 (BSU10680)	gerPE	1148744..1149145 (-)	402	NP_388949.1	spore germination protein	-
BSU_10690 (BSU10690)	gerPD	1149145..1149321 (-)	177	NP_388950.1	spore germination protein	-
BSU_10700 (BSU10700)	gerPC	1149318..1149935 (-)	618	NP_388951.1	spore germination protein	-
BSU_10710 (BSU10710)	gerPB	1149958..1150191 (-)	234	NP_388952.2	spore germination protein	-
BSU_10720 (BSU10720)	gerPA	1150206..1150427 (-)	222	NP_388953.1	spore germination protein	-
BSU_10730 (BSU10730)	pchI	1150850..1151020 (-)	171	NP_388954.1	Spo0A-P phosphatase (iron-metabolism related)	-
BSU_10740 (BSU10740)	yisJ	1151166..1152089 (-)	924	NP_388955.1	putative spore coat protein, CotH paralog	-

Sequence

Protein

• Length: 1130 a.a.
• Molecular weight: 128920.56 Da
• Isoelectric Point: 5.3147

>NTDB_id=641 BSU_10650 NP_388946.2 1144749..1148141(+) (sbcC) [Bacillus subtilis subsp. subtilis str. 168]
MKPIALSIKGLHSFREEQTIDFEGLSGAGVFGIFGPTGSGKSSILDAMTLALYGKVERAANNTHGILNHAEDTLSVSFTF
ALQTNHQISYKVERVFKRTDEMKVKTALCRFIEIKDEHTVLADKASEVNKRVEELLGLTIDDFTRAVVLPQGKFAEFLSL
KGAERRHMLQRLFNLEQYGDRLVKKLRRQAQEANARKNEMLAEQSGLGEASSEAVEQAEKVLEQAEARLEAMRKNRDQAK
ERFTEHQEIWNVQKEKSTYEEEEKRLAEEQPHIDSMQKRLLEAETAAALKPYADRYAEAIQHEEQAEKEQTLAQKDLADR
TAFFQQKHEEYEAWRQHKSEKEPELLAKQEQLSRLQEIEIKLSEAKQEEERKKADLRQKEEALQSVMNELETVTDRLTRG
QNRQTELKQQLKSLQVTSDERKSCQQAAEMALRIRQTEEQIKKEKKRSEELNLVLQKMNEEKNTLVQKTEAEENNIIQAY
EAVQTVYHLVCETERSLTRMTEEARKSQHTLHLQREKARVALLTKELAQKLTAGKPCPVCGSTDHDPSASVHETYEADSH
LEEDIKRTDVLLTEAAALSQEILSAKIMLEEQSARFIEQCPFLQTIQAQNLEAAASFEHQPVYEAFETAKFEWKRIKQDI
LSVKTRMAQMIGAYQESLKKAEQLNEKIGFEKREADRIESIISELQSSMDSSLNMFKEAFQNQSVDEAEKWQQAIEEKDR
AAEECEKRIEKSIAFLAEHEAQKEKLRESGHRLEREKLELHYAAERIKSVIADYEHELGDYAKGDSIQIKLRSVQQDLKL
LKEKEQSLYEELQSAQMKLNQAKSRASASELTLQEAKGRLEKAKAAWLEHTKNTSITRTEEVEQSLIPADELEKMKTGID
QFMDKLKQNAANLKRVAEILAGRALSESEWNETVAALQEAEDAFGAAIEEKGAAAKALAVIRDHHKRFNEIEAELKKWQM
HIDRLDKLQAVFKGNTFVEFLAEEQLESVARDASARLSMLTRQRYAIEVDSEGGFVMRDDANGGVRRPVSSLSGGETFLT
SLSLALALSAQIQLRGEYPLQFFFLDEGFGTLDQDLLDTVVTALEKLQSDNLAVGVISHVQELRARLPKKLIVHPAEPSG
RGTRVSLELM

Nucleotide

• Length: 3393 bp

>NTDB_id=641 BSU_10650 NP_388946.2 1144749..1148141(+) (sbcC) [Bacillus subtilis subsp. subtilis str. 168]
ATGAAGCCGATCGCCTTAAGCATTAAGGGGCTCCACAGCTTTAGAGAGGAGCAGACGATAGATTTTGAAGGCCTTTCCGG
TGCCGGTGTTTTCGGCATTTTCGGCCCGACAGGAAGCGGTAAATCCTCTATACTCGACGCAATGACGCTTGCTTTATACG
GAAAGGTGGAACGGGCGGCGAATAATACGCACGGAATCTTAAATCACGCCGAAGATACGCTGTCTGTGTCCTTTACCTTT
GCGCTTCAGACGAATCACCAAATCTCATACAAAGTCGAGCGTGTGTTTAAGAGAACGGATGAAATGAAGGTAAAAACGGC
ACTTTGCCGCTTCATCGAAATCAAGGACGAGCATACGGTGCTGGCTGATAAAGCCAGCGAAGTGAATAAAAGAGTGGAGG
AGCTCTTAGGGCTGACGATCGACGATTTTACGAGAGCGGTGGTGCTGCCCCAAGGGAAATTTGCTGAATTTCTGTCTTTA
AAAGGGGCAGAGCGCAGGCATATGCTTCAGCGTTTATTTAATTTGGAGCAATATGGAGACAGGCTTGTGAAAAAGCTGAG
ACGGCAGGCGCAGGAAGCCAATGCGAGAAAAAATGAAATGCTTGCTGAACAGTCCGGTCTCGGTGAGGCGAGCTCAGAGG
CAGTGGAGCAGGCTGAAAAGGTTCTCGAACAAGCTGAAGCCCGGCTGGAAGCGATGAGGAAGAACCGTGATCAGGCGAAG
GAGCGGTTTACAGAGCATCAGGAGATATGGAATGTCCAAAAGGAAAAATCCACTTATGAAGAAGAGGAAAAACGTCTCGC
AGAAGAACAGCCGCATATAGACAGCATGCAAAAACGCCTGCTGGAAGCAGAAACAGCAGCAGCCCTTAAGCCCTATGCGG
ACCGGTACGCAGAAGCGATCCAGCATGAGGAGCAAGCTGAAAAGGAACAAACGCTAGCCCAAAAGGATTTAGCAGACCGG
ACAGCTTTCTTTCAGCAAAAACATGAAGAGTATGAAGCGTGGCGCCAGCATAAAAGCGAGAAAGAGCCTGAGCTTTTAGC
CAAACAGGAACAGCTTTCACGCTTGCAGGAAATCGAAATCAAACTGAGTGAGGCCAAGCAAGAGGAAGAGCGCAAAAAGG
CTGACCTCCGGCAGAAAGAAGAGGCTCTTCAATCTGTCATGAATGAATTAGAGACCGTAACAGACCGCCTGACACGAGGG
CAAAACAGACAGACAGAATTGAAGCAGCAGCTCAAATCCCTGCAGGTGACATCCGATGAGCGAAAAAGCTGCCAGCAGGC
CGCAGAGATGGCATTGCGCATCAGACAAACCGAGGAACAAATCAAAAAAGAGAAAAAACGAAGTGAAGAATTGAACCTCG
TGCTGCAGAAGATGAATGAAGAGAAGAATACACTCGTTCAAAAGACGGAAGCGGAAGAAAACAACATCATTCAGGCATAT
GAGGCAGTTCAAACTGTGTACCATTTGGTGTGCGAAACGGAACGCTCATTAACACGTATGACGGAAGAGGCTAGAAAGAG
TCAACACACGCTTCACTTACAGCGTGAAAAAGCAAGGGTGGCACTGCTGACAAAAGAGTTAGCCCAAAAGCTGACTGCCG
GAAAGCCTTGCCCGGTATGCGGTTCAACCGATCATGATCCATCTGCCTCGGTACATGAAACGTATGAAGCCGACAGCCAT
CTTGAAGAGGACATCAAACGGACAGATGTGTTATTGACGGAAGCTGCAGCTCTCAGCCAGGAGATTCTTTCAGCCAAAAT
TATGCTTGAAGAACAGTCCGCGCGCTTTATTGAACAGTGTCCGTTTTTGCAGACAATTCAAGCACAGAACCTTGAAGCGG
CAGCTTCCTTCGAACATCAGCCGGTGTATGAAGCATTTGAAACTGCCAAATTTGAATGGAAACGAATCAAGCAGGACATT
CTTTCTGTTAAGACACGAATGGCACAAATGATTGGCGCCTATCAGGAGTCTTTAAAAAAGGCCGAGCAGCTTAATGAAAA
AATCGGTTTTGAAAAAAGAGAAGCCGACCGTATTGAAAGCATCATCAGTGAGCTTCAATCCTCAATGGACAGCAGTCTGA
ACATGTTTAAAGAAGCATTTCAGAATCAATCTGTGGACGAAGCAGAAAAATGGCAGCAAGCCATTGAAGAAAAGGACCGG
GCTGCAGAAGAATGTGAAAAACGAATTGAGAAGAGTATCGCGTTTCTTGCTGAGCATGAAGCACAAAAGGAAAAACTGCG
GGAATCGGGACACCGGCTTGAGCGGGAAAAGCTGGAGCTTCATTATGCGGCTGAACGCATCAAGAGCGTGATAGCTGATT
ATGAGCACGAACTCGGAGATTATGCAAAAGGAGATTCGATTCAAATCAAACTCCGCTCTGTCCAGCAGGATCTAAAGCTG
TTAAAGGAAAAAGAACAATCTTTATATGAAGAACTGCAAAGCGCCCAAATGAAGCTCAACCAAGCGAAAAGCCGCGCTTC
TGCAAGCGAGCTCACTCTTCAAGAGGCGAAGGGCAGATTGGAAAAAGCAAAAGCTGCTTGGCTTGAGCATACAAAAAACA
CCTCCATTACCCGGACTGAGGAGGTTGAACAAAGTCTCATCCCAGCTGATGAACTTGAAAAGATGAAAACCGGCATAGAC
CAGTTTATGGATAAACTGAAGCAAAATGCTGCAAACTTAAAACGAGTAGCAGAGATACTTGCCGGCAGAGCATTATCAGA
GAGCGAATGGAACGAAACCGTTGCAGCATTACAAGAAGCTGAGGACGCATTTGGCGCTGCTATAGAGGAAAAAGGCGCGG
CCGCAAAAGCACTGGCTGTCATTCGCGACCATCATAAACGGTTTAATGAAATTGAAGCTGAACTGAAAAAATGGCAGATG
CATATCGACAGGCTGGACAAGCTGCAAGCTGTGTTTAAAGGCAATACCTTCGTCGAATTTTTAGCTGAGGAGCAGCTTGA
AAGCGTTGCGAGGGACGCCTCAGCAAGACTCAGTATGCTGACAAGACAGCGCTATGCCATCGAAGTAGATTCTGAGGGCG
GCTTCGTGATGCGGGATGACGCGAATGGAGGCGTACGACGCCCGGTTTCCAGTTTGTCTGGAGGAGAGACCTTCCTCACC
TCGCTTTCACTTGCTCTTGCGCTGTCTGCGCAGATTCAGCTTCGGGGGGAATACCCGCTGCAGTTCTTTTTCTTAGATGA
AGGCTTCGGCACACTGGATCAAGATCTGCTTGATACGGTTGTAACGGCCTTGGAAAAACTTCAGTCAGACAACCTGGCTG
TCGGTGTCATCAGCCATGTGCAGGAACTGCGTGCACGGCTTCCGAAAAAGCTGATCGTCCATCCGGCTGAACCGAGCGGC
CGCGGTACGCGGGTATCACTTGAGTTGATGTAA

Domains

Predicted by InterProScan.

• AAA domain (AAA_23) (6-235)

• SbcC/RAD50-like, Walker B motif (SbcC_Walker_B) (1002-1085)

3D structure

• AlphaFold DB: O06714

References

• [1]: Judita Mascarenhas et al. (2006) Bacillus subtilis SbcC protein plays an important role in DNA inter-strand cross-link repair. BMC Molecular Biology 7:20. [PMID: 16780573]