Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CG450_RS20465 Genome accession   NZ_CP022477
Coordinates   3918398..3918844 (+) Length   148 a.a.
NCBI ID   WP_003178810.1    Uniprot ID   A0A8B5YEZ5
Organism   Bacillus licheniformis strain BL-010     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3913398..3923844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG450_RS20445 - 3914540..3915280 (+) 741 WP_003178817.1 ribonuclease YeeF family protein -
  CG450_RS20450 - 3915396..3917099 (+) 1704 WP_003178816.1 methyl-accepting chemotaxis protein -
  CG450_RS20455 - 3917119..3917922 (-) 804 WP_003178814.1 serine/threonine protein kinase -
  CG450_RS20460 - 3917928..3918131 (-) 204 WP_003178812.1 hypothetical protein -
  CG450_RS20465 nucA/comI 3918398..3918844 (+) 447 WP_003178810.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CG450_RS20470 nin/comJ 3918862..3919260 (+) 399 WP_003178808.1 competence protein ComJ Regulator
  CG450_RS20475 - 3919299..3920735 (-) 1437 WP_003178806.1 glycoside hydrolase family 1 protein -
  CG450_RS20480 - 3920805..3921530 (-) 726 WP_003178804.1 AAA family ATPase -
  CG450_RS20485 - 3921627..3922178 (-) 552 WP_003178803.1 NAD(P)H-dependent oxidoreductase -
  CG450_RS20490 - 3922362..3923237 (+) 876 WP_003178801.1 transporter substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16224.12 Da        Isoelectric Point: 4.9954

>NTDB_id=239921 CG450_RS20465 WP_003178810.1 3918398..3918844(+) (nucA/comI) [Bacillus licheniformis strain BL-010]
MDKIKSLLITLLAVAVVIIGIVSGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHIRKAIKKGHSEICTIDRDGAD
ERRKDSLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISPSDNRGAGSWVGNQVSDYPDGTKVLFKIN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=239921 CG450_RS20465 WP_003178810.1 3918398..3918844(+) (nucA/comI) [Bacillus licheniformis strain BL-010]
ATGGACAAGATTAAATCTTTGCTGATCACCTTGCTCGCCGTCGCCGTCGTGATCATCGGTATTGTATCTGGCGACTTTTT
CACTTCAGAACACCAGCCTTCGGGCCGGCAAAACGGCTCAAACTACGATGAAGTGCTGATTTTTCCTTCGGACCGCTATC
CTGAAACAGGCGCACACATTCGAAAGGCTATCAAAAAAGGGCACTCCGAAATCTGTACGATCGACAGGGACGGAGCCGAC
GAACGCCGAAAGGATTCATTGAAAGATGTTCCTTCCAAATCAGGCTATGACCGCGACGAGTGGCCGATGGCCATGTGCGA
AGAAGGCGGAACAGGTGCTTCTGTCGAGTATATCAGCCCGTCTGATAACCGTGGAGCAGGATCTTGGGTGGGCAATCAGG
TAAGCGATTATCCGGACGGGACGAAAGTACTTTTCAAGATTAATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B5YEZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

64.626

99.324

0.642

Multiple sequence alignment