Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CFN77_RS11670 Genome accession   NZ_CP022319
Coordinates   2281922..2282992 (-) Length   356 a.a.
NCBI ID   WP_096881623.1    Uniprot ID   -
Organism   Bacillus altitudinis strain SGAir0031     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2276922..2287992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN77_RS11620 (CFN77_11740) sipW 2277017..2277589 (-) 573 WP_017357979.1 signal peptidase I SipW -
  CFN77_RS11625 (CFN77_11745) tapA 2277639..2278166 (-) 528 WP_056705241.1 amyloid fiber anchoring/assembly protein TapA -
  CFN77_RS11630 (CFN77_11750) - 2278419..2278745 (+) 327 WP_058336653.1 DUF3889 domain-containing protein -
  CFN77_RS11635 (CFN77_11755) - 2278780..2278974 (-) 195 WP_008342191.1 YqzE family protein -
  CFN77_RS11640 (CFN77_11760) - 2279035..2279418 (-) 384 WP_024718845.1 competence type IV pilus minor pilin ComGG -
  CFN77_RS11645 (CFN77_11765) comGF 2279415..2279912 (-) 498 WP_096881619.1 competence type IV pilus minor pilin ComGF -
  CFN77_RS11650 (CFN77_11770) comGE 2279854..2280168 (-) 315 WP_096881620.1 competence type IV pilus minor pilin ComGE -
  CFN77_RS11655 (CFN77_11775) comGD 2280152..2280598 (-) 447 WP_096881621.1 competence type IV pilus minor pilin ComGD -
  CFN77_RS11660 (CFN77_11780) comGC 2280591..2280884 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  CFN77_RS11665 (CFN77_11785) comGB 2280901..2281941 (-) 1041 WP_096881622.1 competence type IV pilus assembly protein ComGB -
  CFN77_RS11670 (CFN77_11790) comGA 2281922..2282992 (-) 1071 WP_096881623.1 competence type IV pilus ATPase ComGA Machinery gene
  CFN77_RS11675 (CFN77_11795) - 2283451..2284323 (-) 873 WP_096881624.1 STAS domain-containing protein -
  CFN77_RS11685 (CFN77_11805) - 2284548..2284928 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  CFN77_RS11690 (CFN77_11810) - 2285141..2285383 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  CFN77_RS11695 (CFN77_11815) - 2285432..2286070 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  CFN77_RS11700 (CFN77_11820) - 2286231..2286404 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  CFN77_RS11705 (CFN77_11825) - 2286429..2286743 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40383.74 Da        Isoelectric Point: 9.2169

>NTDB_id=238319 CFN77_RS11670 WP_096881623.1 2281922..2282992(-) (comGA) [Bacillus altitudinis strain SGAir0031]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKRFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTVNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=238319 CFN77_RS11670 WP_096881623.1 2281922..2282992(-) (comGA) [Bacillus altitudinis strain SGAir0031]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGTTCATTGGCT
GTGATGTTAAGGCGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAACGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACGTTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGGTGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGGTTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.045

100

0.64

Multiple sequence alignment