Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CFN12_RS08605 Genome accession   NZ_CP022290
Coordinates   1678228..1678914 (-) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain EDCC5458     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1673228..1683914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN12_RS08585 (CFN12_08550) rpsT 1674142..1674393 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  CFN12_RS08590 (CFN12_08555) holA 1674438..1675412 (-) 975 WP_001282562.1 DNA polymerase III subunit delta -
  CFN12_RS08595 (CFN12_08560) comEC 1675469..1677670 (-) 2202 WP_001566051.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CFN12_RS08600 (CFN12_08565) - 1677675..1678136 (-) 462 WP_000439693.1 ComE operon protein 2 -
  CFN12_RS08605 (CFN12_08570) comEA 1678228..1678914 (-) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  CFN12_RS08610 (CFN12_08575) - 1678954..1679670 (-) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  CFN12_RS08615 (CFN12_08580) rsfS 1679673..1680026 (-) 354 WP_001088022.1 ribosome silencing factor -
  CFN12_RS08620 (CFN12_08585) yqeK 1680027..1680611 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CFN12_RS08625 (CFN12_08590) nadD 1680601..1681170 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  CFN12_RS08630 (CFN12_08595) yhbY 1681173..1681463 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  CFN12_RS08635 (CFN12_08600) aroE 1681467..1682273 (-) 807 WP_000666761.1 shikimate dehydrogenase -
  CFN12_RS08640 (CFN12_08605) yqeH 1682287..1683387 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=238058 CFN12_RS08605 WP_001792568.1 1678228..1678914(-) (comEA) [Staphylococcus aureus strain EDCC5458]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=238058 CFN12_RS08605 WP_001792568.1 1678228..1678914(-) (comEA) [Staphylococcus aureus strain EDCC5458]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969

Multiple sequence alignment