Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CEW87_RS11975 Genome accession   NZ_CP022188
Coordinates   2607788..2609242 (-) Length   484 a.a.
NCBI ID   WP_108973284.1    Uniprot ID   -
Organism   Parazoarcus communis strain TSNA42     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2602788..2614242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEW87_RS11950 (CEW87_11935) - 2603220..2604041 (-) 822 WP_234421521.1 substrate-binding periplasmic protein -
  CEW87_RS11955 (CEW87_11940) ilvA 2604263..2605783 (-) 1521 WP_108973276.1 threonine ammonia-lyase, biosynthetic -
  CEW87_RS11960 (CEW87_11945) hprK 2605897..2606859 (-) 963 WP_108973278.1 HPr(Ser) kinase/phosphatase -
  CEW87_RS11965 (CEW87_11950) ptsN 2606828..2607313 (-) 486 WP_108973280.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CEW87_RS11970 (CEW87_11955) hpf 2607447..2607770 (-) 324 WP_108973282.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CEW87_RS11975 (CEW87_11960) rpoN/rpoN1 2607788..2609242 (-) 1455 WP_108973284.1 RNA polymerase factor sigma-54 Machinery gene
  CEW87_RS11980 (CEW87_11965) lptB 2609270..2609992 (-) 723 WP_108973286.1 LPS export ABC transporter ATP-binding protein -
  CEW87_RS11985 (CEW87_11970) recX 2609979..2610428 (-) 450 WP_108973288.1 recombination regulator RecX -
  CEW87_RS11990 (CEW87_11975) recA 2610458..2611495 (-) 1038 WP_108950968.1 recombinase RecA Machinery gene
  CEW87_RS11995 (CEW87_11980) - 2611524..2612042 (-) 519 WP_108973290.1 CinA family protein -
  CEW87_RS12000 (CEW87_11985) - 2612047..2612538 (-) 492 WP_234418930.1 phosphatidylglycerophosphatase A -
  CEW87_RS12005 (CEW87_11990) thiL 2612531..2613490 (-) 960 WP_108973292.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 54135.90 Da        Isoelectric Point: 4.6491

>NTDB_id=237491 CEW87_RS11975 WP_108973284.1 2607788..2609242(-) (rpoN/rpoN1) [Parazoarcus communis strain TSNA42]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNQEIERFLLENPMLEREDGDGSDFAPAAVGNDAQPATETPRETSTET
TQEAEPRNENESADFDEGMDWSGTGSGASSKSDDDEDSDFQEFQAAGTSLRDYLDQQVALSPLSDRDRLLVRFIIEALDS
DGYLHQELEDLLELLPPELDYDLDDLAIALRHVQQLEPAGIGARTPQECLGLQLRAMPAGSCRDLALVIVEQHLELLAER
NFAKLKRLTGCDDDALRDAQALVCRLDPHPGSQHSNEETRYVLPDVVVRKIRNRWTVSLNAEAMPRLRINQLYASLLQQN
RGQGGGLTGQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKYMAT
PRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDAKIADLLGQQGIVVARRTIAKYRESLNIPPVSL
RKAL

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=237491 CEW87_RS11975 WP_108973284.1 2607788..2609242(-) (rpoN/rpoN1) [Parazoarcus communis strain TSNA42]
ATGAAACCGACCCTTCAGCTCAAGCTCTCGCAGCACCTGACGCTCACTCCGCAGCTTCAGCAGTCCATCAAACTGCTGCA
GCTCTCCACGCTCGAGCTCAACCAGGAAATCGAACGCTTTCTGCTGGAAAACCCGATGCTCGAGCGCGAAGACGGCGACG
GCAGCGACTTCGCACCGGCCGCTGTCGGCAACGATGCGCAGCCCGCAACCGAGACCCCGCGCGAGACCAGCACCGAGACC
ACCCAGGAAGCCGAGCCCCGCAACGAGAACGAATCGGCCGACTTTGACGAAGGCATGGACTGGTCGGGCACCGGCAGCGG
CGCCAGCAGCAAGAGCGACGACGACGAGGACAGCGACTTCCAGGAGTTCCAGGCCGCCGGCACCTCGCTGCGCGACTATC
TCGACCAGCAGGTTGCGCTGTCCCCCCTGTCGGACCGTGACCGCCTGCTGGTGCGCTTCATCATCGAAGCACTCGATAGC
GACGGCTACCTGCACCAGGAACTTGAAGACCTGCTCGAACTGCTCCCGCCCGAGCTCGACTACGACCTCGACGACCTCGC
GATTGCCCTGCGACATGTGCAGCAGCTCGAACCGGCGGGGATTGGTGCACGCACCCCGCAGGAGTGTCTCGGCCTGCAGC
TGCGGGCCATGCCTGCAGGCAGCTGCCGTGACCTGGCGCTGGTCATTGTCGAACAGCACCTCGAACTGCTGGCCGAACGA
AACTTTGCGAAGCTGAAGCGCCTCACCGGGTGCGACGACGACGCCCTGCGCGATGCCCAGGCGCTGGTCTGCCGACTCGA
CCCGCACCCGGGCTCACAGCATTCCAACGAAGAAACCCGTTACGTGCTGCCCGATGTCGTCGTGCGCAAGATCCGCAACC
GGTGGACGGTGTCGCTCAATGCCGAAGCCATGCCACGCCTGCGCATCAATCAGCTCTACGCGAGCCTGCTGCAGCAGAAC
CGCGGCCAGGGCGGCGGCCTCACCGGCCAGCTGCAGGAAGCACGCTGGCTGATCAAGAACGTACAACAGCGCTTCGATAC
CATTTTGCGTGTATCTCAGGCCATCGTTGACCAGCAGCGACAGTTCTTCGATCATGGCGAGGTGGCAATGCGTCCATTGA
CCCTCAGGGAGATCGCGGACCAGCTCGAGCTGCATGAATCCACCGTATCGCGTGTCACCACTCAGAAGTACATGGCGACA
CCGCGCGGCGTATTCGAACTCAAGTATTTTTTCGGCAGTCACGTTGCCACTGACACCGGTGGTGCGGCCTCCTCTACGGC
GATTCGTGCATTGATTCGCCAGCTGGTGGATGCCGAAGACAGGAAGAAACCCCTGTCCGACGCAAAGATTGCCGATCTGT
TGGGCCAGCAGGGTATCGTGGTCGCGCGGCGCACAATCGCCAAATACCGCGAATCACTCAATATCCCGCCGGTCAGTCTG
CGTAAAGCGCTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(120-313)

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-482)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

55.952

100

0.583

Multiple sequence alignment