Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   UN84_RS06850 Genome accession   NZ_CP022134
Coordinates   1443610..1444461 (-) Length   283 a.a.
NCBI ID   WP_046078535.1    Uniprot ID   -
Organism   Halomonas sp. HG01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1438610..1449461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UN84_RS06820 - 1439914..1440291 (+) 378 WP_035593549.1 histone-like nucleoid-structuring protein, MvaT/MvaU family -
  UN84_RS06825 - 1440377..1441171 (-) 795 WP_046078531.1 helix-turn-helix transcriptional regulator -
  UN84_RS06830 - 1441483..1441782 (+) 300 WP_046078532.1 hypothetical protein -
  UN84_RS06835 - 1441812..1442684 (-) 873 WP_046078533.1 DNA replication terminus site-binding protein -
  UN84_RS06840 yacG 1442689..1442919 (-) 231 WP_035593542.1 DNA gyrase inhibitor YacG -
  UN84_RS06845 coaE 1442963..1443592 (-) 630 WP_046078534.1 dephospho-CoA kinase -
  UN84_RS06850 pilD 1443610..1444461 (-) 852 WP_046078535.1 A24 family peptidase Machinery gene
  UN84_RS06855 nagZ 1444639..1445652 (+) 1014 WP_046078536.1 beta-N-acetylhexosaminidase -
  UN84_RS06860 - 1445688..1446266 (+) 579 WP_035593534.1 hypoxanthine-guanine phosphoribosyltransferase -
  UN84_RS06865 - 1446349..1446789 (-) 441 WP_046078537.1 DUF411 domain-containing protein -
  UN84_RS06870 - 1446943..1448634 (-) 1692 WP_082090830.1 AMP-binding protein -
  UN84_RS06875 - 1448711..1449154 (-) 444 WP_035593526.1 MerR family DNA-binding transcriptional regulator -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30699.94 Da        Isoelectric Point: 7.5557

>NTDB_id=237146 UN84_RS06850 WP_046078535.1 1443610..1444461(-) (pilD) [Halomonas sp. HG01]
MPVDLPPSLLWPLAAIVGLCLGSFLNVVITRLPVMLMREWRAEAREALELEAEDTTTFNLATPRSMCPRCEAPIAWHDNL
PLIGWLKRRGRCARCGTRISPQYPLVELAGALLTLAVTALFGPTLEGLFVLGACLSLLAMAAIDLRTQLLPDALTLPLLW
AGLLYQLLFQPLQLPSAVIGAMAGYLSLWGFYWLFKLVTGKEGMGYGDFKLFAALGAWLGWVYLPLVLILAAGAGAIVGV
IVQLAVPRLRGAPLPFGPWLAAAGWIALLVGEPLMAHYLTLMM

Nucleotide


Download         Length: 852 bp        

>NTDB_id=237146 UN84_RS06850 WP_046078535.1 1443610..1444461(-) (pilD) [Halomonas sp. HG01]
TTGCCTGTCGATCTTCCCCCTAGCCTGCTGTGGCCGCTGGCCGCGATCGTCGGCCTGTGCCTCGGCAGTTTCCTCAACGT
GGTGATCACTCGCCTGCCGGTGATGCTGATGCGGGAGTGGCGGGCCGAGGCCCGCGAGGCGCTGGAGCTCGAGGCGGAAG
ACACCACGACCTTCAACCTGGCCACGCCGCGCTCGATGTGCCCACGTTGCGAGGCCCCCATCGCCTGGCACGACAACCTG
CCGCTGATCGGGTGGCTGAAACGCCGCGGGCGCTGCGCTCGCTGCGGCACCCGCATCAGCCCCCAGTATCCACTGGTGGA
GCTGGCCGGCGCCCTGCTGACGCTCGCGGTGACGGCACTGTTCGGCCCCACCCTCGAGGGGCTGTTCGTCCTCGGCGCCT
GCCTGAGCCTGCTGGCGATGGCGGCCATCGACCTGCGCACCCAGCTGCTGCCGGACGCCCTGACCCTGCCGCTGCTGTGG
GCCGGCCTGCTGTACCAGCTGCTGTTCCAGCCGCTGCAGCTGCCGAGCGCGGTGATCGGCGCCATGGCCGGCTATCTCTC
GCTGTGGGGCTTCTACTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCTACGGCGACTTCAAGCTGTTCGCCG
CGCTGGGCGCCTGGCTGGGCTGGGTCTACCTGCCGCTGGTGCTGATCCTGGCCGCCGGGGCTGGCGCCATCGTCGGCGTG
ATCGTCCAGTTGGCCGTGCCGCGACTGCGCGGCGCCCCCCTGCCCTTCGGCCCCTGGCTCGCCGCGGCCGGCTGGATCGC
GCTGCTGGTCGGTGAGCCGCTGATGGCCCACTACCTGACCCTGATGATGTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(16-119)

Type IV leader peptidase family (Peptidase_A24)

(134-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

50.554

95.76

0.484
  pilD Vibrio campbellii strain DS40M4

46.57

97.88

0.456
  pilD Acinetobacter nosocomialis M2

47.059

96.113

0.452
  pilD Acinetobacter baumannii D1279779

46.691

96.113

0.449
  pilD Neisseria gonorrhoeae MS11

45.185

95.406

0.431

Multiple sequence alignment