Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MS_RS08760 Genome accession   NC_006300
Coordinates   1897391..1897954 (+) Length   187 a.a.
NCBI ID   WP_041640008.1    Uniprot ID   -
Organism   [Mannheimia] succiniciproducens MBEL55E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1892391..1902954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS_RS08745 (MS1914) cpxA 1892931..1894322 (+) 1392 WP_011201074.1 envelope stress sensor histidine kinase CpxA -
  MS_RS08750 (MS1915) - 1894457..1895854 (-) 1398 WP_041640006.1 DASS family sodium-coupled anion symporter -
  MS_RS08755 (MS1916) - 1896233..1897348 (+) 1116 WP_011201076.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  MS_RS08760 (MS1917) pilF 1897391..1897954 (+) 564 WP_041640008.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MS_RS08765 (MS1918) - 1898107..1899081 (+) 975 WP_011201078.1 RodZ domain-containing protein -
  MS_RS08770 (MS1919) ispG 1899094..1900200 (+) 1107 WP_011201079.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MS_RS08775 (MS1920) hisS 1900257..1901540 (+) 1284 WP_011201080.1 histidine--tRNA ligase -
  MS_RS08780 (MS1921) - 1901544..1902158 (+) 615 WP_011201081.1 YfgM family protein -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 21501.10 Da        Isoelectric Point: 7.8016

>NTDB_id=23675 MS_RS08760 WP_041640008.1 1897391..1897954(+) (pilF) [[Mannheimia] succiniciproducens MBEL55E]
MTFKFQQKLTALFSLFISLLLSACSSSPQVNAENLAKQQAAKARIELGLAYLHQQNINQAKQNFDKALEHAPQYYLTHSA
LAYFYQQLGDVKQARIHYQKAIDLDNNQGDVHNNYGTFLCSQGQFEQAYDEFEQALQSPNYYRQTDSYENLALCALSAKN
NERFQRYLTKLEKLDPKRAAKLENISN

Nucleotide


Download         Length: 564 bp        

>NTDB_id=23675 MS_RS08760 WP_041640008.1 1897391..1897954(+) (pilF) [[Mannheimia] succiniciproducens MBEL55E]
ATGACTTTCAAATTTCAACAAAAATTGACCGCACTTTTTTCCTTATTTATTTCACTGTTACTTTCAGCCTGTAGCTCGTC
TCCCCAGGTAAATGCGGAAAATTTAGCTAAACAACAGGCGGCTAAAGCACGTATTGAATTAGGCTTAGCCTATTTACATC
AACAAAATATCAATCAGGCTAAACAAAATTTCGATAAAGCGCTGGAACACGCGCCTCAATATTATTTAACCCATTCGGCA
CTAGCTTATTTTTATCAACAACTGGGTGACGTCAAACAAGCGCGCATCCATTATCAAAAAGCCATTGATTTAGATAATAA
TCAGGGTGACGTACATAACAATTACGGCACTTTTCTCTGTTCGCAAGGACAATTTGAACAAGCTTACGACGAATTCGAAC
AGGCGCTTCAATCACCCAATTATTATCGTCAAACCGATAGCTACGAAAACTTGGCGCTTTGTGCATTATCAGCTAAAAAT
AATGAGCGTTTTCAACGTTATTTAACCAAATTAGAAAAATTGGATCCCAAGCGCGCCGCCAAATTAGAAAATATATCGAA
CTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(98-128)

Tetratricopeptide repeat (TPR_2)

(41-74)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

47.15

100

0.487
  pilF/pilF2 Haemophilus influenzae Rd KW20

51.149

93.048

0.476

Multiple sequence alignment