Detailed information NTG0236680

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CEP86_RS12915
• Genome accession: NZ_CP022097
• Coordinates: 2791241..2792113 (-)
• Length: 290 a.a.
• NCBI ID: WP_011063522.1
• Uniprot ID: Q4K5X2
• Organism: Pseudomonas protegens strain FDAARGOS_307
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2786241..2797113

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CEP86_RS12875 (CEP86_12825)	-	2786474..2787772 (-)	1299	WP_011063529.1	NAD(P)/FAD-dependent oxidoreductase	-
CEP86_RS12880 (CEP86_12830)	-	2787833..2788006 (-)	174	WP_015637073.1	DUF3094 family protein	-
CEP86_RS12885 (CEP86_12835)	-	2788196..2788825 (-)	630	WP_011063528.1	DUF1780 domain-containing protein	-
CEP86_RS12890 (CEP86_12840)	-	2788969..2789436 (+)	468	WP_026019989.1	hypothetical protein	-
CEP86_RS12895 (CEP86_12845)	-	2789433..2790122 (+)	690	WP_011063526.1	energy-coupling factor ABC transporter permease	-
CEP86_RS12900 (CEP86_12850)	-	2790186..2790401 (+)	216	WP_011063525.1	hypothetical protein	-
CEP86_RS12905 (CEP86_12855)	yacG	2790421..2790624 (-)	204	WP_011063524.1	DNA gyrase inhibitor YacG	-
CEP86_RS12910 (CEP86_12860)	coaE	2790621..2791244 (-)	624	WP_011063523.1	dephospho-CoA kinase	-
CEP86_RS12915 (CEP86_12865)	pilD	2791241..2792113 (-)	873	WP_011063522.1	A24 family peptidase	Machinery gene
CEP86_RS12920 (CEP86_12870)	pilC	2792116..2793333 (-)	1218	WP_011063521.1	type II secretion system F family protein	Machinery gene
CEP86_RS12925 (CEP86_12875)	-	2793663..2794079 (+)	417	WP_011063520.1	pilin	-
CEP86_RS12930 (CEP86_12880)	-	2794308..2794664 (+)	357	WP_011063519.1	BON domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32053.26 Da
• Isoelectric Point: 7.0083

>NTDB_id=236680 CEP86_RS12915 WP_011063522.1 2791241..2792113(-) (pilD) [Pseudomonas protegens strain FDAARGOS_307]
MPLTDALTLYPMAFVAAALVLGLIVGSFLNVLVWRLPKMLERDWRAQARDVLGLGPEPVAAPYNLMLPHSQCPHCAQAIR
PWENIPLLSYLWLRGCCAHCNSSISPRYPLTELACGLISGFIAWHFGFGWAAGLMLILSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANSFGLLISLEDALWGTVIGYLFLWSVFWLFKLLTGKEGMGHGDFKLLALFGAWGGWQILPLTLLLSSLLG
AILGTILLRLRREKTSTPIPFGPFLAIAGWIALLWGGQITDFYLQFVGFQ

Nucleotide

• Length: 873 bp

>NTDB_id=236680 CEP86_RS12915 WP_011063522.1 2791241..2792113(-) (pilD) [Pseudomonas protegens strain FDAARGOS_307]
ATGCCCCTGACTGACGCCCTCACTCTCTACCCCATGGCCTTCGTGGCTGCGGCCCTGGTATTGGGCCTGATCGTCGGCAG
CTTTCTCAACGTGCTGGTGTGGCGCCTGCCGAAAATGCTCGAACGCGACTGGCGAGCCCAGGCCCGCGACGTGCTGGGGC
TGGGGCCGGAACCTGTGGCTGCGCCCTATAACCTGATGCTGCCGCACTCCCAGTGCCCGCACTGTGCGCAGGCGATCCGC
CCCTGGGAAAACATTCCGCTGCTCAGTTACCTGTGGCTGCGTGGCTGCTGCGCCCACTGCAATAGCTCCATCAGCCCGCG
CTACCCGTTGACCGAACTGGCCTGCGGGCTGATCTCGGGTTTTATCGCCTGGCACTTCGGCTTCGGCTGGGCGGCCGGGC
TGATGCTGATCCTCAGTTGGGGGCTGCTGGCAATGAGCCTGATCGACGCCGATCACCAGCTGCTGCCGGATGTGCTGGTG
CTGCCACTGTTGTGGCTGGGGCTGATCGCCAACAGCTTCGGCCTGCTGATCAGCCTGGAGGATGCGCTGTGGGGGACGGT
GATCGGCTACCTGTTCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGCTGACCGGCAAGGAGGGCATGGGCCACGGTGATT
TCAAGCTGCTGGCCCTGTTCGGGGCCTGGGGCGGCTGGCAGATCCTGCCTCTGACCCTGCTGCTGTCATCCCTGTTGGGA
GCGATTCTCGGCACGATCCTGCTGCGCCTGCGCCGGGAAAAGACCTCGACGCCAATCCCCTTTGGACCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTTCTATCTGCAGTTTGTCGGTTTCCAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: Q4K5X2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.317	95.862	0.521
pilD	Acinetobacter baumannii D1279779	51.46	94.483	0.486
pilD	Acinetobacter nosocomialis M2	51.46	94.483	0.486
pilD	Vibrio campbellii strain DS40M4	49.645	97.241	0.483
pilD	Neisseria gonorrhoeae MS11	47.037	93.103	0.438