Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   C7A04_RS10505 Genome accession   NZ_CP027586
Coordinates   1929431..1930195 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain 2012EL-2448     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1924431..1935195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7A04_RS10485 acpT 1925385..1925972 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  C7A04_RS10490 nikA 1926083..1927657 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  C7A04_RS10495 nikB 1927657..1928601 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  C7A04_RS10500 nikC 1928598..1929431 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  C7A04_RS10505 amiE 1929431..1930195 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  C7A04_RS10510 nikE 1930192..1930998 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  C7A04_RS10515 nikR 1931004..1931405 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  C7A04_RS10520 - 1931525..1931884 (-) 360 WP_000593552.1 type II toxin-antitoxin system HicB family antitoxin -
  C7A04_RS10525 - 1931881..1932111 (-) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  C7A04_RS10530 yhhJ 1932215..1933339 (-) 1125 WP_001216257.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=236323 C7A04_RS10505 WP_001136236.1 1929431..1930195(+) (amiE) [Escherichia coli strain 2012EL-2448]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=236323 C7A04_RS10505 WP_001136236.1 1929431..1930195(+) (amiE) [Escherichia coli strain 2012EL-2448]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment