Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C6991_RS13105 Genome accession   NZ_CP027555
Coordinates   2259000..2259440 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain 2013C-3513     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2254000..2264440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6991_RS13080 aroP 2254998..2256371 (+) 1374 WP_012817732.1 aromatic amino acid transporter AroP -
  C6991_RS13085 ampE 2256414..2257268 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  C6991_RS13090 ampD 2257265..2257816 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C6991_RS13095 nadC 2257904..2258797 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C6991_RS13105 pilA 2259000..2259440 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  C6991_RS13110 pilB 2259450..2260835 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  C6991_RS13115 hofC 2260825..2262027 (+) 1203 WP_000157234.1 protein transport protein HofC -
  C6991_RS13120 guaC 2262062..2263105 (-) 1044 WP_001217338.1 GMP reductase -
  C6991_RS32125 - 2263261..2263305 (-) 45 WP_120795372.1 protein YacM -
  C6991_RS13125 coaE 2263330..2263950 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=235728 C6991_RS13105 WP_000360902.1 2259000..2259440(+) (pilA) [Escherichia coli strain 2013C-3513]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=235728 C6991_RS13105 WP_000360902.1 2259000..2259440(+) (pilA) [Escherichia coli strain 2013C-3513]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377

Multiple sequence alignment