Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   XM38_RS04200 Genome accession   NZ_CP021983
Coordinates   895273..897285 (-) Length   670 a.a.
NCBI ID   WP_080809451.1    Uniprot ID   A0A1Z3HI25
Organism   Halomicronema hongdechloris C2206     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 890273..902285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XM38_RS04185 (XM38_008720) - 890708..892456 (+) 1749 WP_080809459.1 ABC transporter ATP-binding protein -
  XM38_RS04190 (XM38_008730) - 892450..893670 (-) 1221 WP_080809456.1 type II secretion system F family protein -
  XM38_RS04195 (XM38_008740) pilT 894035..895168 (-) 1134 WP_080809454.1 type IV pilus twitching motility protein PilT Machinery gene
  XM38_RS04200 (XM38_008750) pilB/pilB1 895273..897285 (-) 2013 WP_080809451.1 GspE/PulE family protein Machinery gene
  XM38_RS04205 (XM38_008760) grpE 897707..898483 (+) 777 WP_080809449.1 nucleotide exchange factor GrpE -
  XM38_RS04210 (XM38_008770) dnaK 898668..900680 (+) 2013 WP_088429162.1 molecular chaperone DnaK -
  XM38_RS04215 (XM38_008780) dnaJ 900840..901973 (+) 1134 WP_080809445.1 molecular chaperone DnaJ -
  XM38_RS04220 (XM38_008790) - 901970..902272 (+) 303 WP_080809443.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74459.85 Da        Isoelectric Point: 4.9074

>NTDB_id=235600 XM38_RS04200 WP_080809451.1 895273..897285(-) (pilB/pilB1) [Halomicronema hongdechloris C2206]
MTNSSSSRRALVLQRSFSPFGNKLIQAGYVDSDQMQQALVESRKSGRSLTDVLESMTGQQLSPELLRQYKKQQLFELKIL
YGVESLDPEIDSISNQQITQLIDSLIPVDVCRRHRLVPLSRDDDNEVPSVLVAMVDPENLEAQDDLNRILRQQNLKLRRM
VITVEDYQRLISGYLDEKVARESKKELDEAVDVTTSLEELDLNEVDALGTIAEEEADLGEALKDAGAAPVIALVNKILVK
ALQENASDIHVEPQEDHLRVRFRKDGVLREALPKMPKKIIPAVTARFKIIAELDIAERRVPQDGRIRRAFQNRKVDFRVS
TLPSRFGEKVVLRILDNTSTQLGLDNLITDAETLKVVQEMVSRPYGLILVTGPTGSGKTTTLYSALAERNSPTINISTAE
DPIEYTLAGITQVQVLREKGMDFASILRAFLRQDPDVILVGETRDPETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDE
MGVESFMVSSALIGVLAQRLMRRVCTNCRIAYSPSVEDLARFGLTASRESEITFYKANTLSPEQITAANQNGTLCRQCQG
GGYSGRVGVYEVMRITENLQKLISEGAPTEQIKELAVDEGMQTLLAYSLNLVRQGYTTLEEVERVTFSDTGLESELKARR
RASLTCSNCGAGLKPEWLDCPYCLTSRFQN

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=235600 XM38_RS04200 WP_080809451.1 895273..897285(-) (pilB/pilB1) [Halomicronema hongdechloris C2206]
ATGACTAACTCCTCTTCATCGCGGCGAGCGCTCGTTCTGCAAAGGAGCTTCTCACCCTTCGGCAATAAGCTGATCCAGGC
TGGTTATGTGGACTCGGATCAGATGCAGCAGGCACTGGTTGAGAGCCGCAAATCGGGTCGTAGCCTCACGGATGTGCTGG
AATCGATGACGGGGCAGCAGCTTTCGCCAGAGCTCCTGCGTCAGTATAAGAAGCAGCAACTCTTTGAGTTGAAGATTCTC
TACGGGGTAGAGTCGCTTGATCCAGAAATTGACAGTATTTCCAATCAGCAAATTACCCAGCTGATTGATTCGCTGATTCC
GGTTGACGTCTGTCGCCGCCATCGTCTAGTGCCCTTGTCTCGCGATGATGATAATGAGGTGCCTTCAGTATTGGTGGCGA
TGGTCGATCCTGAGAATTTAGAAGCTCAGGATGATCTAAACCGCATCTTACGTCAGCAAAACTTAAAGTTGCGACGCATG
GTGATCACCGTTGAGGACTATCAACGGCTGATTTCTGGCTACCTGGATGAAAAGGTGGCGCGGGAAAGCAAAAAGGAGCT
GGATGAGGCGGTTGATGTTACCACCAGCTTAGAGGAACTGGATCTCAATGAAGTCGATGCGTTAGGGACCATTGCCGAGG
AAGAGGCTGATCTCGGGGAAGCCCTCAAGGATGCTGGGGCGGCTCCGGTCATTGCCCTAGTCAACAAAATCTTGGTTAAG
GCATTGCAAGAGAATGCCTCCGACATCCATGTGGAGCCTCAAGAAGATCACCTGCGGGTACGGTTCCGCAAGGATGGCGT
CTTGCGGGAAGCCCTGCCCAAAATGCCTAAGAAGATTATTCCAGCAGTGACGGCCCGCTTTAAGATCATTGCTGAGCTGG
ATATCGCGGAGCGTCGGGTACCTCAAGACGGCAGAATCCGACGGGCCTTTCAGAATCGCAAGGTAGACTTTCGGGTTAGC
ACGTTGCCGAGCCGTTTTGGCGAAAAGGTGGTGTTGCGAATCCTGGACAATACCTCCACTCAACTGGGGTTGGATAACCT
GATTACCGATGCAGAGACCTTGAAGGTTGTGCAGGAGATGGTGTCTCGTCCCTATGGTTTGATTTTGGTGACGGGACCCA
CCGGCTCTGGTAAAACCACGACGCTCTATTCGGCCCTGGCCGAGCGCAATAGCCCTACGATTAATATCAGTACGGCGGAA
GATCCGATTGAATATACGCTGGCGGGCATCACGCAGGTACAGGTGCTGCGGGAGAAGGGGATGGATTTTGCCTCTATCCT
GCGGGCCTTTTTGCGACAAGACCCCGATGTGATCTTGGTGGGTGAGACCCGTGACCCCGAAACGGCTAAAACGGCCATTG
AAGCTGCCCTGACGGGGCACTTGGTGCTGACAACGCTCCATACCAATGATGCTGCCGGTGCGATCGCACGTCTTGATGAG
ATGGGCGTAGAATCGTTTATGGTCTCCAGTGCTCTGATTGGGGTACTGGCTCAGCGACTAATGCGCCGTGTTTGCACCAA
TTGTCGCATTGCCTACAGCCCTAGTGTCGAGGATCTGGCGCGATTCGGCCTCACTGCTTCCCGTGAGAGCGAGATTACCT
TCTACAAGGCCAATACCCTTTCTCCCGAACAAATCACGGCTGCCAATCAAAATGGCACCCTCTGCCGCCAGTGCCAGGGC
GGAGGTTACTCTGGCCGGGTAGGGGTTTACGAAGTCATGCGTATTACCGAAAACCTGCAAAAACTCATCTCCGAGGGAGC
CCCAACCGAGCAAATCAAGGAACTTGCTGTTGACGAGGGCATGCAAACGCTGTTGGCCTATAGCCTTAACTTGGTTCGGC
AAGGCTACACCACTCTAGAAGAAGTCGAACGAGTGACCTTCAGCGATACGGGGCTTGAATCTGAGTTGAAGGCCCGGCGC
CGGGCTTCTCTTACCTGCTCCAATTGCGGTGCTGGCCTCAAGCCCGAATGGCTAGATTGCCCCTATTGTCTCACCTCCCG
TTTCCAAAATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3HI25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.471

99.851

0.654
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.08

91.194

0.375

Multiple sequence alignment