Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   S101395_RS18280 Genome accession   NZ_CP021920
Coordinates   3467736..3471425 (-) Length   1229 a.a.
NCBI ID   WP_006636760.1    Uniprot ID   M5P7R6
Organism   Bacillus sonorensis strain SRCM101395     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3462736..3476425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S101395_RS18265 - 3462791..3463009 (+) 219 WP_006636763.1 spore germination protein -
  S101395_RS18270 (S101395_03678) sbcC 3463093..3466485 (-) 3393 WP_006636762.1 exonuclease subunit SbcC -
  S101395_RS18275 (S101395_03679) sbcD 3466482..3467666 (-) 1185 WP_006636761.1 exonuclease subunit SbcD -
  S101395_RS18280 (S101395_03680) addA 3467736..3471425 (-) 3690 WP_006636760.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  S101395_RS18285 (S101395_03681) addB 3471412..3474912 (-) 3501 WP_006636759.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  S101395_RS18290 (S101395_03682) modB 3475022..3475678 (-) 657 WP_006636758.1 molybdate ABC transporter permease subunit -

Sequence

Protein


Download         Length: 1229 a.a.        Molecular weight: 141359.10 Da        Isoelectric Point: 5.4615

>NTDB_id=234910 S101395_RS18280 WP_006636760.1 3467736..3471425(-) (addA) [Bacillus sonorensis strain SRCM101395]
MEMAKPKNSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITAEDNPIDVDRLLVVTFTNASAAEMKHRIGEA
LERELAEHPGSLHLRRQLSLLNKASISTLHSFCLQVVRKYYYMIDVDPAFRIADQTEGELLGDEVLDELFEEEYQKGDPA
FFELVDRYTTDRHDLDLQYLVKRVYEFSRSHPDPESWLEKFVELYDADADAKVEALPFYPYIKEDVELALKGVREKLARA
LELAKQPGGPAPRAENFLDDLAQIDNLIRHQDDFAALYKLVSAVSFQRAKPCKGDEFEPSLIEEATELRNSAKKQLEKLK
TDYFTRTPDQHLASLKEMKPVIKTLVKLVIEFGRRFKAVKKEKAIVDFSDLEHDCLAILTKKNEAGETVPSEAAAHYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGAGQKIDLNKNFRSR
SDILDSTNFLFKQLMGGKVGEVDYDEQAELKLGASYPPNDSTKTEVLMIENQENSADEEAEELETVQLEARAMAGRIREL
ISGKFQVYDGKTKTTRNIEYRDIVILLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVAVTLSLLKIIDNAYQDIPL
ASVLRSPIVGLDENELSLIRIRDKKAPFYEALKAYIASSDPDDTLNEKLSRFDRLLKKWRTYAKHHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYESTSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFLVG
TAKDKEKLLAKWRSSASRSEWLLPDFERFQAKSYLDFIGPALMRHRDMSDDLPSPNDEIYHHPSRFTISWISSAELEAED
QAHTEEEKHDRLSLIKKGLPIDGPFDYEEEVRERLTWSYPYQDASRIRTKQSVSEIKRQKEYEDEYGDRSLIRPAQETVL
FKRPSFMMAKGLTAAERGTAMHTVMQHLPLTHAPQADELGRLLDRLVEKELLTEDQRAAIEEDDILAFFETDIGEKLLSA
RRVEREVPFNMTLPAQDVYPGMNTADEPVLIQGIIDCLFETDDGFYLLDYKTDRIHGKFRSGFEGAEPILRKRYETQINL
YARAVETMLKTPLKGKALYFFDGCHVLMF

Nucleotide


Download         Length: 3690 bp        

>NTDB_id=234910 S101395_RS18280 WP_006636760.1 3467736..3471425(-) (addA) [Bacillus sonorensis strain SRCM101395]
ATGGAGATGGCTAAGCCGAAGAACAGCACATGGACGGATGATCAATGGAAAGCGATTGTCTCATCCGGACGGGACATTCT
TGTCGCGGCGGCCGCCGGTTCCGGAAAAACGGCTGTCCTTGTCGAGCGGATCATCCGGAAAATTACGGCCGAGGATAACC
CGATCGATGTCGACCGGCTGCTTGTCGTCACATTTACAAACGCATCCGCAGCCGAAATGAAGCACAGGATAGGTGAAGCG
CTTGAAAGGGAGCTTGCCGAACATCCCGGCTCCCTTCATTTGCGCAGGCAGCTTTCCTTGCTGAACAAGGCAAGCATTTC
AACCTTGCACTCTTTTTGCCTTCAGGTCGTTCGCAAATATTATTATATGATCGACGTTGATCCGGCCTTTCGGATCGCCG
ACCAGACAGAAGGAGAGCTGCTTGGCGATGAAGTGCTTGACGAGCTGTTTGAGGAAGAATATCAAAAAGGCGATCCGGCT
TTTTTTGAACTGGTTGATCGATATACGACAGACCGCCATGATCTGGATTTGCAGTACCTGGTGAAGCGGGTCTATGAGTT
TTCCCGCTCTCATCCCGATCCGGAATCCTGGCTTGAAAAGTTTGTCGAGCTGTATGACGCAGATGCTGACGCGAAGGTAG
AAGCACTGCCGTTTTATCCTTATATCAAGGAAGATGTCGAACTCGCTCTGAAAGGTGTGCGGGAAAAGCTGGCCCGTGCG
CTTGAGCTGGCAAAACAGCCGGGAGGCCCTGCGCCGCGCGCGGAAAACTTTCTCGATGATTTGGCGCAGATCGACAATCT
GATCAGGCATCAGGATGATTTCGCGGCGCTTTATAAGCTTGTGTCCGCCGTTTCATTTCAACGGGCAAAGCCGTGCAAAG
GAGATGAATTCGAGCCGTCTTTAATCGAAGAAGCGACAGAGCTGCGAAACAGTGCGAAAAAACAGCTGGAAAAGCTGAAA
ACCGATTATTTCACGCGGACTCCTGATCAGCATTTGGCAAGTTTGAAGGAGATGAAGCCCGTCATCAAGACGCTTGTCAA
GCTTGTCATCGAATTCGGCAGACGCTTTAAGGCGGTGAAAAAAGAAAAGGCCATCGTCGATTTCTCCGATCTTGAACACG
ACTGTCTGGCGATTTTGACAAAGAAAAATGAAGCGGGGGAAACGGTCCCGAGCGAAGCTGCGGCCCATTACCGGCATCAG
TTCCATGAAGTGCTTGTCGATGAGTACCAGGATACAAACCTTGTCCAGGAGGCCATCTTAAAGCTGGTCGCCAAGGAGGA
GCATGAGGGAAATCTCTTTATGGTGGGCGATGTCAAGCAGTCGATCTACCGATTCCGCCTGGCTGAGCCGCTGTTATTCC
TCTCAAAATATAAACGGTTTACAGATGATGGATCAGGCGCCGGACAGAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCCGACATTTTAGACAGCACCAACTTTCTGTTTAAGCAGCTTATGGGGGGAAAGGTCGGCGAAGTTGACTATGACGAGCA
GGCAGAATTAAAGCTCGGCGCGTCATATCCTCCAAATGACAGCACAAAAACGGAAGTGCTGATGATTGAAAATCAGGAAA
ACAGCGCGGATGAAGAGGCGGAAGAGCTGGAAACGGTTCAGCTTGAGGCGAGGGCCATGGCAGGACGGATCCGCGAGCTG
ATATCCGGGAAATTCCAAGTGTATGACGGCAAAACGAAAACAACGAGAAACATCGAGTACCGCGACATCGTGATTTTATT
GCGGTCAATGCCGTGGGCGCCGCAGATCATGGATGAATTCAAACAGCAGGGAATTCCGGTTTACGCGAATTTGTCAACAG
GCTATTTTGAAGCGACAGAAGTCGCAGTCACGCTTTCGCTGCTGAAGATAATCGATAATGCATACCAGGATATCCCGCTG
GCATCCGTTCTCAGATCGCCGATTGTCGGCCTTGATGAAAATGAGCTGTCCCTGATCAGAATCAGGGATAAAAAAGCTCC
TTTTTATGAAGCGCTGAAAGCGTATATCGCCTCGTCCGACCCGGATGACACCCTTAATGAAAAGCTGAGCCGCTTTGACC
GGCTCCTCAAAAAGTGGAGGACATATGCAAAACATCATTCAGTGGCTGAGCTGATCTGGGAAATTTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAGCAGCGGCAGGCCAACTTGCGCGCGCTTTACGACAGGGCCAGATC
ATATGAATCGACATCCTTTCGCGGCTTGTTCCGCTTTTTGAGATTTATAGAACGAATGCAGGAGCGGGGCGATGATCTCG
GAACCGCAAGAGCTTTGAGCGAACAGGAAGACGTCGTCCGGCTGATGACGATTCATTCAAGCAAAGGGCTTGAGTTTCCA
GTCGTATTTACGGCAGGACTTGGCCGGAATTTTAACATGATGGACTTAAACAAATCATATCTGCTTGATAAAGAGCTCGG
TTTCGGGACAAAGTTTATCCATCCAAAATGGCGGATCAGTTATCCGACGCTGCCGCTTATCGCAATGAAGAAAAAAATGC
GCCGCGAGCTTTTATCAGAAGAGCTCCGCGTCCTGTATGTCGCCCTAACCCGGGCAAAGGAAAAGCTGTTTTTGGTCGGT
ACGGCGAAGGACAAGGAAAAGCTATTGGCAAAATGGCGGTCTTCCGCTTCCCGATCCGAATGGCTGCTGCCTGATTTTGA
ACGTTTTCAGGCCAAATCCTATCTCGATTTTATCGGGCCCGCATTAATGCGGCACAGGGATATGTCTGACGATTTACCGT
CGCCGAATGATGAAATCTATCATCATCCGTCCCGTTTTACGATCAGCTGGATCTCTTCCGCAGAGCTTGAAGCTGAAGAT
CAGGCTCACACCGAGGAGGAAAAGCATGACCGGCTGTCACTGATTAAAAAAGGGCTGCCGATCGATGGACCATTTGATTA
TGAGGAGGAAGTGCGCGAGAGGCTGACATGGTCATACCCTTATCAAGACGCGTCCCGCATCCGCACAAAGCAGTCTGTCT
CCGAAATCAAAAGGCAGAAGGAATATGAGGATGAATATGGCGACCGATCATTGATTCGCCCGGCACAAGAGACTGTATTG
TTCAAAAGGCCTTCCTTTATGATGGCGAAAGGGCTGACGGCTGCCGAACGGGGAACGGCGATGCATACGGTCATGCAGCA
CCTCCCGTTGACCCATGCCCCGCAAGCGGATGAGCTTGGCCGCCTGCTTGACAGGCTTGTCGAGAAAGAGCTTTTAACAG
AAGATCAGCGTGCGGCGATCGAAGAAGACGATATTCTCGCGTTTTTCGAAACGGACATAGGTGAAAAACTGCTGAGCGCC
CGGCGGGTTGAACGGGAAGTTCCCTTCAATATGACGCTGCCCGCCCAGGACGTATATCCCGGCATGAACACAGCGGATGA
ACCTGTATTGATTCAGGGGATTATTGACTGTTTGTTTGAGACGGATGATGGATTTTATTTGCTCGATTACAAAACAGACC
GGATACACGGCAAATTTAGAAGCGGCTTTGAAGGAGCGGAGCCGATACTGAGAAAACGCTATGAAACACAGATCAATCTC
TATGCGCGGGCCGTCGAAACGATGTTGAAGACGCCTCTGAAAGGAAAGGCCCTTTACTTTTTTGACGGCTGCCATGTGCT
AATGTTTTGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(471-880)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1050-1221)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5P7R6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

73.089

100

0.731

Multiple sequence alignment