Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   S101395_RS00605 Genome accession   NZ_CP021920
Coordinates   110552..111931 (+) Length   459 a.a.
NCBI ID   WP_006640292.1    Uniprot ID   M5PBX4
Organism   Bacillus sonorensis strain SRCM101395     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 105552..116931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S101395_RS00585 (S101395_00116) - 105920..106384 (+) 465 WP_006640288.1 CtsR family transcriptional regulator -
  S101395_RS00590 (S101395_00117) - 106399..106956 (+) 558 WP_006640289.1 UvrB/UvrC motif-containing protein -
  S101395_RS00595 (S101395_00118) - 106956..108047 (+) 1092 WP_006640290.1 protein arginine kinase -
  S101395_RS00600 (S101395_00119) clpC 108044..110476 (+) 2433 WP_006640291.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  S101395_RS00605 (S101395_00120) radA 110552..111931 (+) 1380 WP_006640292.1 DNA repair protein RadA Machinery gene
  S101395_RS00610 (S101395_00121) disA 111935..113011 (+) 1077 WP_006640293.1 DNA integrity scanning diadenylate cyclase DisA -
  S101395_RS00615 (S101395_00122) - 113141..114229 (+) 1089 WP_006640294.1 PIN/TRAM domain-containing protein -
  S101395_RS00620 (S101395_00123) ispD 114245..114940 (+) 696 WP_006640295.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  S101395_RS00625 (S101395_00124) ispF 114933..115409 (+) 477 WP_006640296.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49878.26 Da        Isoelectric Point: 7.1320

>NTDB_id=234864 S101395_RS00605 WP_006640292.1 110552..111931(+) (radA) [Bacillus sonorensis strain SRCM101395]
MAKTKTKFICQSCGYESPKWMGKCPGCGAWNTMVEETIKKPAANRRTAFSHSVQRVQKPSPISSIETSEEPRIKTNLGEF
NRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADLNENVLYISGEESVKQTKLRADRLGINSSTLHVLSETDMEYIT
SSIEEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRLAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMIIPAANLEGWTKPKGIEVVGVENVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=234864 S101395_RS00605 WP_006640292.1 110552..111931(+) (radA) [Bacillus sonorensis strain SRCM101395]
ATGGCTAAAACAAAGACTAAATTTATCTGTCAATCATGCGGCTATGAATCTCCAAAATGGATGGGGAAATGCCCGGGCTG
CGGCGCATGGAACACGATGGTCGAAGAAACGATTAAAAAACCGGCCGCCAACAGAAGAACTGCATTTTCTCATTCCGTTC
AAAGGGTTCAAAAACCGTCGCCCATCTCATCAATCGAAACGTCAGAAGAACCGCGGATCAAGACGAATCTCGGCGAATTT
AACCGCGTGCTGGGAAATGGCATCGTCAAAGGCTCGCTTGTCTTAATCGGCGGTGATCCAGGCATCGGAAAATCGACTTT
GCTGCTCCAGGTTTCCGCGCAGCTTGCCGATTTAAATGAAAACGTCCTGTACATATCAGGGGAGGAATCTGTCAAACAAA
CGAAATTAAGAGCGGACCGGTTAGGCATCAACAGTTCAACTCTGCACGTTTTATCTGAAACCGATATGGAGTATATTACG
TCTTCTATAGAAGAGATGAATCCTTCTTTTGTTGTGGTGGATTCAATTCAAACCGTTTATCAAAGCGACATTACCTCAGC
TCCGGGCAGCGTATCCCAAGTGAGGGAATGCACCGCGGAGCTGATGAGACTCGCCAAAACAAAAGGAATTCCGATATTTA
TCGTCGGACATGTTACGAAAGAAGGCTCGATTGCCGGGCCGAGACTGTTGGAGCATATGGTCGACACCGTCCTTTACTTT
GAAGGGGAAAGGCATCACACTTTCCGGATTTTAAGAGCCGTCAAAAACCGATTCGGCTCGACAAATGAAATGGGAATATT
TGAGATGAGGGAAGAGGGCCTTACTGAGGTGCTGAACCCGTCGGAAATTTTTCTTGAAGAACGCTCGGCGGGAGCGGCCG
GGTCAAGTGTTGTAGCATCAATGGAAGGAACGAGGCCGGTTCTGGTCGAAATTCAGGCGCTCATTTCACCTACAAGCTTC
GGAAATCCGCGGAGAATGGCCACCGGCATTGATCATAACCGCGTCTCGCTGCTCATGGCTGTATTGGAAAAAAGAGTCGG
CCTGCTGCTTCAAAATCAGGATGCCTATTTAAAAGTCGCCGGCGGAGTAAAGCTTGATGAACCGGCGATAGATCTTGCCG
TGGCGGTCAGCATTGCTTCAAGCTTTAGAGATACACCTCCCCATCCGACGGATTGTTTTATCGGCGAGGTGGGCCTTACC
GGAGAAGTCCGCAGAGTATCTAGAATCGAACAGCGGGTTCAGGAAGCGGCAAAGCTTGGGTTCAAAAGAATGATTATTCC
CGCCGCAAATTTGGAAGGATGGACCAAACCGAAAGGAATTGAGGTCGTCGGTGTTGAAAATGTAGCTGAGGCTCTTCGAA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5PBX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

91.703

99.782

0.915
  radA Streptococcus mitis NCTC 12261

63.135

98.693

0.623
  radA Streptococcus pneumoniae Rx1

63.135

98.693

0.623
  radA Streptococcus pneumoniae D39

63.135

98.693

0.623
  radA Streptococcus pneumoniae R6

63.135

98.693

0.623
  radA Streptococcus pneumoniae TIGR4

63.135

98.693

0.623
  radA Streptococcus mitis SK321

62.914

98.693

0.621

Multiple sequence alignment