Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CDO84_RS20860 Genome accession   NZ_CP021911
Coordinates   4094415..4095791 (+) Length   458 a.a.
NCBI ID   WP_015252992.1    Uniprot ID   A0A0C3LIP7
Organism   Bacillus sp. MD-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4089415..4100791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDO84_RS20840 (CDO84_20840) ctsR 4089768..4090232 (+) 465 WP_014662512.1 transcriptional regulator CtsR -
  CDO84_RS20845 (CDO84_20845) mcsA 4090246..4090803 (+) 558 WP_014475591.1 protein-arginine kinase activator protein McsA -
  CDO84_RS20850 (CDO84_20850) - 4090803..4091894 (+) 1092 WP_040081043.1 protein arginine kinase -
  CDO84_RS20855 (CDO84_20855) clpC 4091891..4094323 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  CDO84_RS20860 (CDO84_20860) radA 4094415..4095791 (+) 1377 WP_015252992.1 DNA repair protein RadA Machinery gene
  CDO84_RS20865 (CDO84_20865) disA 4095795..4096877 (+) 1083 WP_014662516.1 DNA integrity scanning diadenylate cyclase DisA -
  CDO84_RS20870 (CDO84_20870) - 4096993..4098093 (+) 1101 WP_014662517.1 PIN/TRAM domain-containing protein -
  CDO84_RS20875 (CDO84_20875) ispD 4098108..4098806 (+) 699 WP_040081040.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CDO84_RS20880 (CDO84_20880) ispF 4098799..4099275 (+) 477 WP_014475594.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49438.94 Da        Isoelectric Point: 8.2399

>NTDB_id=234844 CDO84_RS20860 WP_015252992.1 4094415..4095791(+) (radA) [Bacillus sp. MD-5]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=234844 CDO84_RS20860 WP_015252992.1 4094415..4095791(+) (radA) [Bacillus sp. MD-5]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTTGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTTAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATCGGCGGTGATCCTGGTATCGGAAAGTCAACGTT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTTCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTTCGAGCTGACCGCCTCGGCATCAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTATACCAAAGCGATATCACATCGGC
ACCGGGCAGCGTGTCACAGGTCAGAGAATGTACCGCTGAGCTAATGAAAATTGCAAAAACAAAAGGTATCCCGATTTTTA
TCGTGGGACACGTGACGAAAGAAGGGTCTATTGCAGGGCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGTCACCATACTTTCCGCATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATATT
TGAAATGCGGGAAGAGGGGCTGACTGAGGTTTTGAATCCTTCAGAAATTTTCTTAGAGGAACGCTCTGCGGGTTCCGCGG
GCTCGAGTATCACTGCTTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGAAATCCAAGGCGTATGGCAACGGGAATAGACCACAACAGGGTTTCTCTGTTAATGGCTGTATTAGAAAAAAGAGTGGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATCAGTATCGCTTCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTGCGACGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCTAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C3LIP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.563

100

0.996
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624

Multiple sequence alignment