Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   AAF93_RS28115 Genome accession   NZ_CP027472
Coordinates   5177459..5178118 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2014C-3050     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 5172459..5183118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAF93_RS28090 parE 5172550..5174442 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  AAF93_RS28095 ygiN 5174490..5174804 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  AAF93_RS28100 mdaB 5174835..5175416 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  AAF93_RS28105 ygiZ 5175735..5176067 (+) 333 WP_000912120.1 DUF2645 family protein -
  AAF93_RS28110 qseC 5176113..5177462 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  AAF93_RS28115 ciaR 5177459..5178118 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  AAF93_RS28120 ygiW 5178270..5178662 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  AAF93_RS28125 ygiV 5178715..5179197 (+) 483 WP_000183500.1 GyrI-like domain-containing protein -
  AAF93_RS28135 parC 5179743..5182001 (+) 2259 WP_001281899.1 DNA topoisomerase IV subunit A -
  AAF93_RS28140 plsC 5182234..5182971 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=234844 AAF93_RS28115 WP_001221502.1 5177459..5178118(-) (ciaR) [Escherichia coli strain 2014C-3050]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=234844 AAF93_RS28115 WP_001221502.1 5177459..5178118(-) (ciaR) [Escherichia coli strain 2014C-3050]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment