Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   CDO84_RS04845 Genome accession   NZ_CP021911
Coordinates   992065..995769 (+) Length   1234 a.a.
NCBI ID   WP_040083514.1    Uniprot ID   -
Organism   Bacillus sp. MD-5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 987065..1000769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDO84_RS04830 (CDO84_04830) - 987509..987838 (-) 330 WP_052438172.1 four-helix bundle copper-binding protein -
  CDO84_RS04835 (CDO84_04835) - 987957..988394 (-) 438 WP_040083516.1 ferritin family protein -
  CDO84_RS04840 (CDO84_04840) addB 988578..992078 (+) 3501 WP_040083515.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CDO84_RS04845 (CDO84_04845) addA 992065..995769 (+) 3705 WP_040083514.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CDO84_RS04850 (CDO84_04850) sbcD 995841..997016 (+) 1176 WP_040083512.1 exonuclease subunit SbcD -
  CDO84_RS04855 (CDO84_04855) sbcC 997013..1000405 (+) 3393 WP_040083511.1 exonuclease subunit SbcC -
  CDO84_RS04860 (CDO84_04860) hlpB 1000419..1000721 (+) 303 WP_014663462.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141338.63 Da        Isoelectric Point: 5.0653

>NTDB_id=234779 CDO84_RS04845 WP_040083514.1 992065..995769(+) (addA) [Bacillus sp. MD-5]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVQSPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVPEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSELYRRVPAVAFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLTEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAQNDKGEYEPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEDTDANEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLHTFYGHLQKWRVFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGSALARHRDLEALAGNGTPVHADISGHPARFAVQMIHSYD
LLDDDVEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLNWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRALVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVQRLYEKELLTEEQKDAIDIEEIVQFFHTEIGG
QLIGAKWKDREIPFSLALPAKEIYPDAHEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYD
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=234779 CDO84_RS04845 WP_040083514.1 992065..995769(+) (addA) [Bacillus sp. MD-5]
ATGAACATTCCTAAACCGGCAGATAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCAGAGGAAAACC
CAATTGATGTAGACCGCCTTCTTGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGAATTGCGGATGCC
TTGGAAAAAGAGCTTGTACAAAGCCCCGGCTCGCTGCATATCAGACGGCAGCTTTCTCTTTTGAACCGGGCCAGCATTTC
GACGCTTCATTCCTTTTGCCTGCAAGTGCTGAAAAAATATTACTACTTGATCGATCTTGATCCGGGTTTTCGGATAGCTG
ATCAGACGGAAGGCGAGCTGCTTGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTCCAATTTCTCGTTAAACAAGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGAGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTACCAGAAAAGAGCGCCATCG
AGGAGCTACCGTTCTATCAATATGTCAAAGAGGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTAGCGCAGATTGATGAACT
GATTCAGCACCAGGACGATTTTAGTGAACTATATCGTCGTGTGCCAGCCGTCGCTTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCGGCGCTCCTTGAAGAGGCGACAGATTTGAGGAACGGCGCAAAAAAACTGCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGTCCTGAACAGCACTTGAAAAGCCTAACCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GCTTGTTATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTCAGAATGACAAAGGTGAATATGAGCCGAGCGAGGCTGCAAGGTTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTACAGGAATCGATTCTGCAGCTCGTCACAAGCGGGCC
GGAGGAGACTGGCAACCTGTTTATGGTAGGCGATGTCAAACAGTCAATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGGGAAGGCACGGGACGGAAAATCGACTTGAATAAAAATTTCCGAAGC
CGTTCAGATATATTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCAGCGTATCCGGACAATGACGAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAACGAGGAAGCAGAAGAGCTTGAAACCGTGCAGTTTGAGGCCAAAGCCATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACATCGCAATATTCAATACCGAGATATTGTGAT
TTTGCTCCGCTCCATGCCGTGGGCTCCGCAAATCATGGAAGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTCGAAGCGGTCGAAGTCGCCGTCGCGCTTTCAGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGACTACCTGGCTGCTGGTGACCGGAACGATGAGCTTTATCAAAAGCTGCATACGT
TTTACGGACATCTGCAAAAATGGCGCGTCTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATATATGGACTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGACAGGGC
GCGCCAATATGAATCAACGGCATTTCGCGGCTTGTTTCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGCCGGAATTTTAACATGATGGATTTAAATAAATCGTACCTGCTGGACAAGGA
GCTCGGATTCGGCACGAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACACTGCCTCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGAATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAAACTGATTGGCTCCTTCCGGA
ATTTGACCGCTATCAGGCGAAAACATATTTAGATTTCATCGGATCGGCTCTTGCCAGACATAGAGACTTGGAGGCTTTGG
CTGGAAACGGTACACCAGTACACGCTGATATCTCAGGCCATCCAGCTCGTTTTGCCGTTCAAATGATTCATTCCTATGAT
TTGCTTGATGATGATGTGGAAGAAAGAATGGAAGAAAAAAGTGAACGTCTGGAAGCGATCCGCCGAGGGGAACCAGTTCC
CGGCTCGTTTGCATTTGATGAAAAAGCCCGCGAGCAGCTGAACTGGACCTACCCGCATCAAGAGGTGACACAAATTCGGA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCTTGTAAAACCGGCT
GACGGAAGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGTACTGCCATGCA
TACGGTGATGCAGCATATCCCGTTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCAAAGGCTTTATGAAA
AAGAGCTGCTTACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGGGGA
CAGCTGATCGGTGCGAAATGGAAGGACCGGGAAATACCTTTCAGCTTGGCGCTCCCGGCTAAGGAGATCTATCCTGATGC
ACACGAGGCGGATGAGCCGCTTTTAGTGCAGGGGATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATTTATTGG
ATTATAAGTCGGACCGGATTGAGGGCAAATTCCAACACGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAT
ACCCAAATTCAGCTGTACACGAAAGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTCACATTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

97.488

100

0.975

Multiple sequence alignment