Detailed information NTG0234778

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: CDO84_RS04840
• Genome accession: NZ_CP021911
• Coordinates: 988578..992078 (+)
• Length: 1166 a.a.
• NCBI ID: WP_040083515.1
• Uniprot ID: -
• Organism: Bacillus sp. MD-5
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 983578..997078

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CDO84_RS04820 (CDO84_04820)	-	984429..985631 (-)	1203	WP_040083518.1	MFS transporter	-
CDO84_RS04825 (CDO84_04825)	-	985745..987472 (+)	1728	WP_040083517.1	SgrR family transcriptional regulator	-
CDO84_RS04830 (CDO84_04830)	-	987509..987838 (-)	330	WP_052438172.1	four-helix bundle copper-binding protein	-
CDO84_RS04835 (CDO84_04835)	-	987957..988394 (-)	438	WP_040083516.1	ferritin family protein	-
CDO84_RS04840 (CDO84_04840)	addB	988578..992078 (+)	3501	WP_040083515.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
CDO84_RS04845 (CDO84_04845)	addA	992065..995769 (+)	3705	WP_040083514.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
CDO84_RS04850 (CDO84_04850)	sbcD	995841..997016 (+)	1176	WP_040083512.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134704.60 Da
• Isoelectric Point: 5.5198

>NTDB_id=234778 CDO84_RS04840 WP_040083515.1 988578..992078(+) (addB) [Bacillus sp. MD-5]
MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKNGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSFTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTQTPELAHLEAQYEVRPAVPYAEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPRAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVHEKAEALYRYLEETEVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVVKRLEELFPHHKERLLTNEPEQVSDEEQLKYVVNQS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=234778 CDO84_RS04840 WP_040083515.1 988578..992078(+) (addB) [Bacillus sp. MD-5]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCAACAGCATTCAGGATGAATTACG
CCGGGCTCCATTCGGGAAGCCAATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTTCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACAAGCACCGGCGTACAAATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAACGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGGTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAATATCGCGGAGAACGTGTGTTATCTGAA
AAGCTCCATGACTTATCGATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCGGAAGATGTAAAAGGCGCGCATATCTATGTGGATGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGCTGGAGCAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGTTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTAGACATTACGTATAAAGAGCTGAGTGGGACTGAGCGGCATACACAGACACCTGAATTGGCCCATCTAG
AGGCGCAGTATGAAGTGCGTCCGGCCGTTCCTTACGCAGAAAAACAAGAAGCACTGACTGTAATGCAGGCCGCGAACAGA
CGAGCTGAGCTGGAAGGTATTGCCCGGGAAATTCACGCCCTCGTCAGAGAGAAAGGATATCGCTATAAGGATGTAGCAAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCAAGCCTTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGCTATTCCCGCTCAATGAACCGAGGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCTTATGGGATCAAAGGCGACCGCTGGACAAAGGGCGACCGTTTCCAATATAGGCGCT
TTGTATCATTGGATGATGATTTCGCGCAGACCGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGAATGAAAAAAGCAAAGACGGTACATGAGAAGGCGGAGGCGCTCTATCG
CTATTTAGAAGAGACGGAGGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGACGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCGGAGTCGCTTACATTTTCGCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACATCCTGTACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTTCTGTCGGATGATGACCGGGAATGGCTGAAAACGATCGGGGTTGAGCTTTCC
TCAGGCGGACGGGAGCGTTTGCTTGATGAGCATTTCCTCATCTACATGGCATTTTCAAGTCCGTCTGACCGGCTTTATGT
ATCGTATCCGATTGCAGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCGTTAAACGGCTGGAAGAACTGTTTCCGC
ATCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTGAAGTATGTTGTCAATCAAAGC
GTGGCACAGTCCTTTACTGCGAGTCAGCTCAGATTATGGACCCGGGAATATGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGTGTGTCAAGACAGCTCTATGGTGAACGCATTCAGGGCAGCGTATCGAGAATGGAAACCTTTAACGCA
TGTCCGTTTTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTTGAAGCGCCGGATATTGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACAGGCTGCGTGAGCAAAAGCTGGATTGGAGAGATTTAACGAAAG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTAGAGCGGTTGGCACCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTAAAGGAAAAACTGCAAAAAATTGTGACGCGTGTGTCCGGTATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTGGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGTATTGACCGTGTGGATAAAGCTGAAAGCTCAAAAGGCCTGCTCCTCAGAATTGTC
GATTATAAATCAAGCGATAAAGGGCTTGACTTAGCGGAAGTATATTACGGATTGGCGCTGCAAATGCTGACGTACCTTGA
TTTATCGATTACACATTCAGCCGACTGGCTCGGAATGAGGGCAACGCCTGCAGGAGTGCTGTATTTCCATATTCATGATC
CGATGATTCAATCTAATCTCCCGCTCGGGCTTGACGAGATTGAACAGGAGATTTTTAAGAAATTTAAAATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGGTCAAATATCATCAACGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCAGACTCGGCAGCAGTCGGGGAAAAGGAATTTGACCTTTTGACAAAGCATGTGCGCC
GTACCTTCCAAGAAGCAGGCGAACAAATTACCGACGGCCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACACCG
TGCACATACTGTGCGTTCAAATCAGTGTGCCAATTTGATGAATCACTAGAGGAAAACGAGTATCGCCCATTAAAAGCTGA
AAAAGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (306-668)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	98.714	100	0.987