Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CD795_RS08030 Genome accession   NZ_CP021907
Coordinates   1609912..1610409 (-) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain Seattle 1945 isolate G478     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1604912..1615409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CD795_RS08005 gcvPB 1605074..1606546 (-) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  CD795_RS08010 gcvPA 1606539..1607885 (-) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  CD795_RS08015 gcvT 1607905..1608996 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  CD795_RS08020 - 1609155..1609679 (-) 525 WP_001015125.1 shikimate kinase -
  CD795_RS08025 - 1609669..1609815 (-) 147 WP_001789879.1 hypothetical protein -
  CD795_RS08030 comGF 1609912..1610409 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  CD795_RS08035 comGE 1610327..1610626 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  CD795_RS08040 comGD 1610613..1611059 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  CD795_RS08045 comGC 1611037..1611348 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  CD795_RS08050 comGB 1611362..1612432 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  CD795_RS08055 comGA 1612404..1613378 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  CD795_RS08060 - 1613430..1614053 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  CD795_RS08065 - 1614050..1614379 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CD795_RS08070 - 1614379..1615365 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=234718 CD795_RS08030 WP_001794328.1 1609912..1610409(-) (comGF) [Staphylococcus aureus strain Seattle 1945 isolate G478]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=234718 CD795_RS08030 WP_001794328.1 1609912..1610409(-) (comGF) [Staphylococcus aureus strain Seattle 1945 isolate G478]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976

Multiple sequence alignment